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Apoptosis Induction of MCF-7 Human Breast Carcinoma Cells by Butein  

Song, Ba-Da (Department of Preventive Meidicine, Cancer Preventive, Kyung Hee University)
Kim, Sun-Rye (Department of Preventive Meidicine, Cancer Preventive, Kyung Hee University)
Kim, Sung-Hun (Material Development Research Center, College of Oriental Medicine, Kyung Hee University)
Shin, Yong-Cheol (Department of Preventive Meidicine, Cancer Preventive, Kyung Hee University)
Ko, Seong-Gyu (Department of Preventive Meidicine, Cancer Preventive, Kyung Hee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.3, 2010 , pp. 385-389 More about this Journal
Abstract
Butein(3,4,2',4-tetrahydroxychalcone) has been reported anticancer effects in several cancer type, which is prostate, bladder cancer but breast cancer is not. This study was to investigate the antiproliferative effects by butein(3,4,2',4-tetrahydroxychalcone) in MCF-7 human breast carcinoma cells. We invastigated the effects of dose-dependently cell growth inhibition by butein, which could be proved by WST-1 assay. Also, flow cytometry analysis was butein increase percentage of subG1 phase. As well as, butein induces apoptosis through the expression of caspase-8,-3 and poly(ADP-ribose) polymerase(PARP) activation but not in DMSO treated cells. Taken together, this results suggest that butein induced MCF-7 apoptosis through extrinsic pathway and thus may have potential tumor suppressor in breast cancer.
Keywords
Butein(3,4,2',4-tetrahydroxychalcone); apoptosis; extrinsic pathway; PARP(poly(ADP-ribose) polymerase);
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