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Inhibitory Effect of Dioscorea Bulbifera MeOH Extract on Pro-inflammatory Mediator In Vitro and In Vivo  

Jung, Ji-Yun (College of Oriental Medicine, Daegu Haany University)
Lee, Jong-Rok (College of Oriental Medicine, Daegu Haany University)
Byun, Sung-Hui (College of Oriental Medicine, Daegu Haany University)
Jung, Ji-Wook (Department of Herbal Medicinal Pharmacology, Daegu Haany University)
Kim, Yong-Han (Department of Herbal Medicinal Pharmacology, Daegu Haany University)
Kim, Sang-Chan (College of Oriental Medicine, Daegu Haany University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.2, 2010 , pp. 310-318 More about this Journal
Abstract
Dioscorea bulbifera is one of the traditional medicinal herb. It commonly used in the treatment of hematemesis, epistaxis, tuberculous cervical lymphadenitis, laryngitis, acute infectious disease in East Asia. In the present study, we have demonstrated the anti-inflammatory effects of Dioscorea bulbifera MeOH extract (DBME) in macrophage cell line. To investigate mechanism of the anti-inflammatory activity, we examined the effects of the lipopolysaccaride (LPS)-induced production of nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), pro-inflammatory cytokines and expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), p-inhibitory ${\kappa}B{\alpha}$ (p-$I{\kappa}B{\alpha}$), and nuclear factor-${\kappa}B$ (NF-${\kappa}B$) in a murine macrophage cell line RAW 264.7. The RAW 264.7 cells were cultured in DMEM + serum medium for 24 hrs. After serum starvation for 24 hrs, the cells were treated with DBME 0.03, 0.10, 0.30 mg/$m{\ell}$ for 1 h, followed by stimulation with LPS (1 ${\mu}g/m{\ell}$) for activation of immune response. After treatment, cell viability was measured by MTT assay, and NO production was monitored by measuring the nitrite content in culture medium. The protein band of iNOS, COX-2, p-$I{\kappa}B{\alpha}$, and NF-${\kappa}B$ was determined by immunoblot analysis and levels of cytokine were analyzed by sandwich immunoassays. There were three experimental groups: carrageenan, DBME 0.3, 1.0 g/kg. Rats were administrated either carrageenan (40% PEG) or carrageenan + DBME (0.3, 1.0 g/kg body weight) for 4 days (p.o.). To induce acute paw edema, rats were injected 1% carrageenan (100 ${\mu}{\ell}$/rat, dissolved in sterilized saline). The effect of DBME in the carrageenan-induced rat paw edema. As results, DBME has an inhibitory effect on the production of NO, PGE2, TNF-${\alpha}$, IL-$1{\beta}$ and IL-6 and on the expression of iNOS, COX-2, p-$I{\kappa}B{\alpha}$ and translocation of NF-${\kappa}B$ to nuclear from cytosol. In addition, DBME effectively inhibited the increases of paw edema induced by carrageenan treatment in vivo. These results suggest that DBME can inhibit production of pro-inflammatory mediators and might be a useful source for treatment of acute inflammatory disease.
Keywords
dioscorea bulbifera; LPS; cytokines; carrageenan; paw edema;
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1 Palmer, R.M., Ashton, D.S., Moncada, S. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature. 333: 664-666, 1988.   DOI   ScienceOn
2 Weider, N., Semple, J.P., Folkman, J. Tumor angiogenesis and metastasis-correlation in invasive breast carcinoma. N Engl J Med. 324(1):1-8, 1991.   DOI   ScienceOn
3 Stamler, J.S., Singel, D.J., Loscalzo, J. Biochemistry of nitric oxide and its redox-activated forms. Science. 258: 1898-1902, 1992.   DOI
4 Lee, A.K., Sung, S.H., Kim, Y.C., Kim, S.G. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, $TNF-{\alpha}$, COX-2 expression by sauchinone effects on $I-{\kappa}B{\alpha}$ phosphorylation, C/EBP and AP-1 activation. British journal of pharmacology. 139(1):11-20, 2003.   DOI   ScienceOn
5 Ryu, J.H., Ahn, H., Kim, J.Y., Kim, Y.K. Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophage. Phytother. Res. 17(5):485-489, 2003.   DOI   ScienceOn
6 Weis, Z.A., Cicatiello, L., Esumi, H. Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-gamma, bacterial lipopolysaccharide and NG-monomethy1-L-araginine. Biochem. J. 316: 209-215, 1996.   DOI
7 Papayianni, A. Cytokine, growth factors, and other inflammatory mediators in glomerulonephritis. Ren Fail. 18(5):725-774, 1996.   DOI   ScienceOn
8 Choi, W.Y., Jo, M.J., Kim, S.C., Jung, J.Y. Aucklandiae Radix has inhibitory effects of pro-inflammatory mediator in LPS-induced RAW 264.7 cell. The Journal of Jeahan Oriental Medical Academy. 7(1):1-12, 2009.
9 Byun, S.H., Yang, C.H., Kim, S.C. Inhibitory effect of Sctophulariae Radix extract on $TNF-\alpha$, $IL-1\beta$, IL-6 and Nitric Oxide production in lipoposaccharide-activated RAW 264.7 cells. Kor. J. Herbology. 20(2):7-16, 2005.
10 Aggarwal, B.B., Kohr, W.J., Hass, P.E., Moffat, B., Spencer, S.A., Henzel, W.J., Bringman, T.S., Nedwin, G.E., Goeddel, D.V., Harkins, R.N. Human tumor necrosis factor. Production purification and characterization. J Biol Chem. 260(4):2345-2354, 1985.
11 Mu, M.M., Chakravortty, D., Sugiyama, T., Koide, N., Takahashi, K., Mori, I., Yoshida, T., Yokochi, T. The inhibitory acrtion of quercetin on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells. J. Endotoxin Res. 7(6):431-438, 2001.   DOI
12 Mcdaniel, M.L, Kwon, G., Hill, J.R., Marshall, C.A., Corbett, J.A. Cytokines and nitric oxide in islet inflammation and diabetes. Proc Soc Exp Biol Med. 211(1):24-32, 1996.   DOI
13 Chen, F., Castranova, V., Shi, X. New insights into the role of nuclear factor-kappa B in cell growth regulation. Ann J Pathhol. 159(2):387-397, 2001.
14 Kim, S.H., Ryu, B.H., Ryu, K.W., Yoon, S.H., Kim, J.S. An anti-tumor effects of Dioscorea bulbifera L. on stomach cancer cells. Korean J. Orient Int Med. 23(1):99-106, 2002.   과학기술학회마을
15 Gaidamshvili, M., Ohizumi, Y., Iijima, S., Takayama, T., Ogawa, T., Muramoto, K. Characterization of the yam tuber storage proteins from Dioscorea batatas exhibiting unique lectin activities. J. Biol. Chem. 279(25):26028-26035, 2004.   DOI   ScienceOn
16 Van Snick J. Interleukin-6: an overview. Annu Rev. Immunol. 8: 253-278, 1990.   DOI   ScienceOn
17 Liu, S.F., Malik, A.B. NF-$\kappa$ B activation as a pathological mechanism of septic shock and inflammation. Am J. Physiol. Lung Cell Mol. Physiol. 290(4):L622-L645, 2006.   DOI   ScienceOn
18 정보섭, 신민교. 도해 향약 생약 대사전. 서울, 영림사, pp 149-150, 1990.
19 Westacott, C.L., Sharif, M. Cytokines in osteoarthritis: mediators or makers of joint destruction. Semin Arthriris Rheum. 25: 254-327, 1996.   DOI   ScienceOn
20 Vane, J.R., Bakhle, Y.S., Botting, R.M. Cyclooxygenases 1 and 2. Annu Rev Pharmacol Toxicol. 38: 91-120, 1998.
21 McCarteny-Francis, N., Allen, J.B., Mizel, D.E., Albina, J.E., Xie, Q.W., Nathan, C.F., Wahl, S.M. Suppression of arthritis by an inhibitor of nitric oxide synthase. J Exp Med. 178(2):749-754, 1993.   DOI   ScienceOn
22 Bader, A.M., Cook, M.N., Swift, B.A., Newmantraa, T.M., Gowen, M., Lark, M. Inhibition of interleukin-1 induced proteoglycan degradation and nitric oxide production in bovine articular cartilage/chondrocyte cultures by the natural product, hymenialdisine. J Pharmacol Exp Ther. 290(2):587-593, 1999.
23 Seo, Y.J., Na, H.G., Lee, J.S., Cheo, G.S., Joydeb, K., Kundu. Chemopreventive and chemoprotective effects of green tea polyphenol on oxidative and inflammatory tissue damage. International Symposium on Green Tea. pp 103-118, 2003.
24 Gowen, M., Wood, D.D., Ihrie, E.J., Russel, R.G.G. Stimulation by human interleukin 1 of cartilage breakdown and production of collagenase and proteolycanase by human chondrocytes but not by human osteoblast in vitro. Biochem Biophys Acta. 797: 186-193, 1984.   DOI   ScienceOn
25 Park, S.J., Kim, J.Y., Jan, Y.P., Cho, Y.W., Ahn, E.M., Baek, N.I., Lee, K.T. Inhibition of LPS induced iNOS, COX-2 and Cytokines expression by Genistein-4'-O-$\alpha$ -L-Rhamnopyranosyl-(1-2)-$\beta$-D-glucopyranoside through the NF-$\kappa$ B inactivation in RAW 264.7 cells. Kor. J. Pharmacogen. 38(4):339-348, 2007.   과학기술학회마을
26 Willeaume, V., Kruys, V., Mijatovic, T., Huez, G. Tumor necrosis Factor- alpha production induced by viruses and by lipopolysaccharides in macrophages: similarities and differences. J. Inflamm. 46: 1-12, 1995-1996.
27 Isomaki, P., Punnonen, J. Pro-and anti-inflammatory cytokines in rheumatoid arthritis. Annals of Medicine. 29: 449-507, 1997.
28 Willoughby, D.A. Human arthritis applied to animal models. Towards a better therapy. Ann Rheum. Dis. 34: 471-478, 1975.   DOI   ScienceOn