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Inhibitory Effect of Fructus Ligustri Lucidi on Tyrosinase and MITF Expressions  

Han, Gyu-Su (Department of Herbal Resources, Professional Graduate School of Oriental Medicine)
Kim, Dae-Sung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine)
Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine)
Mun, Yeun-Ja (Research Center of Traditional Korean Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.2, 2010 , pp. 296-301 More about this Journal
Abstract
The purpose of this study was to investigate the mechanism of Hexane extract of Fructus Ligustri Lucidi (HFLL)-induced regulation of melanogenesis. An apparent down-regulatory effect of tyrosinase activity was observed when B16F10 cells were incubated with HFLL. Interestingly, HFLL did not inhibit the catalytic activity of cell-free tyrosinase from B16F10 cells, whereas kojic acid directly inhibited tyrosinase activity. Regarding protein levels of melanogenic enzymes, the amounts of tyrosinase and tyrosinase-related protein 1 (TRP-1) were decreased by HFLL, while the amount of tyrosinase-related protein 2 (TRP-2) slightly was reduced after incubation with HFLL. Treatment with HFLL was found to down-regulate microphthalmia-associated transcription factor (MITF). These results suggest that HFLL is an effective inhibitor of pigmentation caused by down regulation via MITF, tyrosinase, and TRP-1 expressions.
Keywords
Fructus Ligustri Lucidi; melanin; tyrosinase; depigmentation; MITF;
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Times Cited By KSCI : 2  (Citation Analysis)
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