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Effect of Artemisiae Argi Folium Fermented with Sacchromyces Cerevisiae on Viability of Human Hepatocyte Treated with Toxicants  

Park, Wan-Su (Department of Pathology, College of Oriental Medicine, Kyungwon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.2, 2010 , pp. 284-289 More about this Journal
Abstract
The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Sacchromyces cerevisiae (AFS) on viability of human hepatocyte HepG2 cells treated with hepatotoxicants such as EtOH, gallic acid, nicotine, acetaminophen, acetaldehyde, and lipopolysaccharide. AFS (0~400 ug/mL) was treated with EtOH, gallic acid, nicotine, acetaminophen, acetaldehyde, and lipopolysaccharide. And the viability of HepG2 cells was measured by MTT assay. AFS showed to increase significantly viabilities of HepG2 cells compared with hepatotoxicants (EtOH, gallic acid, nicotine, acetaminophen, and lipopolysaccharide) only (p<0.05). AFS could be supposed to have the hepatoprotective effect against hepatotoxicants such as gallic acid, EtOH, nicotine, acetaminophen, and lipopolysaccharide.
Keywords
hepatocyte; sacchromyces cerevisiae; artemisiae argi folium; fermentation; cell viability;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Kim, E.Y., Kim, E.K., Lee, H.S., Sohn, Y., Soh, Y., Jung, H.S., Sohn, N.W. Protective effects of Cuscutae semen against dimethylnitrosamine-induced acute liver injury in Sprague-Dawley rats. Biol Pharm Bull. 30(8):1427-1431, 2007.   DOI   ScienceOn
2 Jeong, M.A., Lee, K.W., Yoon, D.Y., Lee, H.J. Jaceosidin, a pharmacologically active flavone derived from Artemisia argyi, inhibits phorbol-ester-induced upregulation of COX-2 and MMP-9 by blocking phosphorylation of ERK-1 and -2 in cultured human mammary epithelial cells. Ann NY Acad Sci. 1095: 458-466, 2007.   DOI   ScienceOn
3 Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 65(1-2):55-63, 1983.   DOI   ScienceOn
4 Ferrari, M., Fornasiero, M.C., Isetta, A.M. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol Methods. 131(2):165-172, 1990.   DOI   ScienceOn
5 Oez, S., Platzer, E., Welte, K. A quantitative colorimetric method to evaluate the functional state of human polymorphonuclear leukocytes. Blut. 60(2):97-102, 1990.   DOI   ScienceOn
6 Gerlier, D., Thomasset, N. Use of MTT colorimetric assay to measure cell activation. J Immunol Methods. 94(1-2):57-63, 1986.   DOI   ScienceOn
7 박완수, 김도훈. 고삼과 애엽의 발효 혼합물이 에탄올과 니코틴으로 유발된 마우스 대식세포 내 hydrogen peroxide 생성 감소에 미치는 영향. 동의생리병리학회지 22(5):1293-1298, 2008.   과학기술학회마을
8 Zhou, S.F., Xue, C.C., Yu, X.Q., Wang, G. Metabolic activation of herbal and dietary constituents and its clinical and toxicological implications: an update. Curr Drug Metab. 8(6):526-553, 2007.   DOI   ScienceOn
9 김호철. 한약약리학. 서울, 집문당, pp 309-311, 2001.
10 전국한의과대학 공동교재편찬위원회. 본초학. 서울, 영림사, pp 405-407, 1991.
11 박완수. Gallic acid 등으로 유발된 대식세포 내 hydrogen peroxide 생성억제에 대한 유산균발효애엽 추출물의 영향. 동의생리병리학회지 23(2):438-442, 2009.   과학기술학회마을
12 한효상, 박완수, 이영종. 艾葉발효 추출물의 면역활성에 관한 연구. 대한본초학회지 23(3):103-112, 2008.   과학기술학회마을
13 Jaeschke, H. Role of inflammation in the mechanism of acetaminophen-induced hepatotoxicity. Expert Opin Drug Metab Toxicol. 1(3):389-397, 2005.   DOI   ScienceOn
14 Kaur, G., Tirkey, N., Chopra, K. Beneficial effect of hesperidin on lipopolysaccharide-induced hepatotoxicity. Toxicology. 226(2-3):152-160, 2006.   DOI   ScienceOn
15 Lee, S.H., Kim, H.K., Seo, J.M., Kang, H.M., Kim, J.H., Son, K.H., Lee, H., Kwon, B.M., Shin, J., Seo, Y. Arteminolides B, C, and D, new inhibitors of farnesyl protein transferase from Artemisia argyi. J Org Chem. 67(22):7670-7675, 2002.   DOI   ScienceOn
16 Tan, R., Jia, Z. Eudesmanolides and Other Constituents from Artemisia argyi. Planta Med. 58(4):370-372, 1992.   DOI   ScienceOn
17 조수인, 김형우, 이근지. 동백 발효 추출물 단기 투여의 활성에 대한 연구. 대한본초학회지 21(2):55-62, 2006.   과학기술학회마을
18 Sheng, H.P., Yuen, S.T., So, H.L., Cho, C.H. Hepatotoxicity of prenatal and postnatal exposure to nicotine in rat pups. Exp Biol Med (Maywood). 226(10):934-939, 2001   DOI
19 Galati, G., Lin, A., Sultan, A.M., O'Brien, P.J. Cellular and in vivo hepatotoxicity caused by green tea phenolic acids and catechins. Free Radic Biol Med. 40(4):570-580, 2006.   DOI   ScienceOn
20 Lieber, C.S. Alcoholic fatty liver: its pathogenesis and mechanism of progression to inflammation and fibrosis. Alcohol. 34(1):9-19, 2004.   DOI   ScienceOn
21 Roesler, J., Hecht, M., Freihorst, J., Lohmann-Matthes, M.L., Emmendörffer, A. Diagnosis of chronic granulomatous disease and of its mode of inheritance by dihydrorhodamine 123 and flow microcytofluorometry. Eur J Pediatr. 150(3):161-165, 1991.   DOI   ScienceOn
22 Kim, M.J., Kim, D.H., Lee, K.W., Yoon, D.Y., Surh, Y.J. Jaceosidin induces apoptosis in ras-transformed human breast epithelial cells through generation of reactive oxygen species. Ann NY Acad Sci. 1095: 483-495, 2007.   DOI   ScienceOn
23 Balderas-Renteria, I., Camacho-Corona, Mdel. R., Carranza- Rosales, P., Lozano-Garza, H.G., Castillo-Nava, D., Alvarez-Mendoza, F.J., Tamez-Cantu, E.M. Hepatoprotective effect of Leucophyllum frutescens on Wistar albino rats intoxicated with carbon tetrachloride. Ann Hepatol. 6(4):251-254, 2007.
24 Lee, H.G., Yu, K.A., Oh, W.K., Baeg, T.W., Oh, H.C., Ahn, J.S., Jang, W.C., Kim, J.W., Lim, J.S., Choe, Y.K., Yoon, D.Y. Inhibitory effect of jaceosidin isolated from Artemisiaargyi on the function of E6 and E7 oncoproteins of HPV 16. J Ethnopharmacol. 98(3):339-343, 2005.   DOI   ScienceOn
25 Adams, M., Efferth, T., Bauer, R. Activity-guided isolation of scopoletin and isoscopoletin, the inhibitory active principles towards CCRF-CEM leukaemia cells and multi-drug resistant CEM/ADR5000 cells, from Artemisia argyi. Planta Med. 72(9):862-864, 2006.   DOI   ScienceOn
26 Lin, H,M., Tseng, H.C., Wang, C.J., Lin, J.J., Lo, C.W., Chou, F.P. Hepatoprotective effects of Solanum nigrum Linn extract against CCl(4)-induced oxidative damage in rats. Chem Biol Interact. 171(3):283-293, 2008.   DOI   ScienceOn
27 Park, W.S. Effect of Sacchromyces cerevisiae-Fermented Artemisiae Argi Folium on Nitric oxide Production of Macrophage Treated with Toxicants. 동의생리병리학회지 23(4):883-887, 2009.   과학기술학회마을
28 Yuan, L.P., Chen, F.H., Ling, L., Dou, P.F., Bo, H., Zhong, M.M., Xia, L.J. Protective effects of total flavonoids of Bidens pilosa L. (TFB) on animal liver injury and liver fibrosis. J Ethnopharmacol. 116(3):539-546, 2008.   DOI   ScienceOn
29 Savvidou, S., Goulis, J., Giavazis, I., Patsiaoura, K., Hytiroglou, P., Arvanitakis, C. Herb-induced hepatitis by Teucrium polium L. report of two cases and review of the literature. Eur J Gastroenterol Hepatol. 19(6):507-511, 2007.   DOI   ScienceOn
30 Saad, B., Azaizeh, H., Abu-Hijleh, G., Said, O. Safety of traditional arab herbal medicine. Evid Based Complement Alternat Med. 3(4):433-439, 2006.
31 Yen, F.L., Wu, T.H., Lin, L.T., Cham, T.M., Lin, C.C. Nanoparticles formulation of Cuscuta chinensis prevents acetaminophen-induced hepatotoxicity in rats. Food Chem Toxicol. 46(5):1771-1777, 2008.   DOI   ScienceOn