Browse > Article

Lonicera Japonica Inhibits Atopy Dermatitis in NC/Nga Mouse through Regulation of iNOS by NF-${\kappa}B$ Suppression  

Ahn, Sang-Hyun (Department of Anatomy, Oriental Medicine College, Dongguk University)
Kim, Ho-Hyun (Department of Physiology, Oriental Medicine College, Semyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.2, 2010 , pp. 278-283 More about this Journal
Abstract
Inducible nitric oxide synthase (iNOS) are important inflammation enzyme and severe up-nitric oxide (NO) production by this enzyme has been intricate with pathogenesis of atopy dermatitis. The present study was designed in order to determine whether Lonicera japonica could inhibit atopy dermatitis through modulation of iNOS by NF-${\kappa}B$ suppression. We found that IKK mRNA and iNOS mRNA expression in RAW 264.7 macrophages stimulated with lipopolysaccharide dose-dependantly decreased by Lonicera japonica (0.4 - 1.0 mg/$m{\ell}$) and NO production decreased. The distribution of NF-${\kappa}B$ p65 and iNOS positive reacted cell in NC/Nga mice with atopy dermatitis were decreased by Lonicera japonica (45 mg/kg/day) and apoptosis were increased. These data likely indicate that Lonicera japonica may act as inflammatory regulator for atopy dermatitis through iNOS modulation by NF-${\kappa}B$B suppression and may be possible to develop useful agent for chemoprevention of NO intricate inflammatory diseases.
Keywords
lonicera japonica; atopy dermatitis; iNOS; NO; apoptosis; NC/Nga mice;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Kwak, W.J., Han, C.K., Chang, H.W., Kim, H.P., Kang, S.S., Son, K.H. Loniceroside C, an Antiinflammatory Saponin from Lonicera japonica. Chem Pharm Bull. 51(3):333-335, 2003.   DOI   ScienceOn
2 Lee, B.G., Kim, S.H., Zee, O.P., Lee, K.R., Lee, H.Y., Han, J.W., Lee, H.W. Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extractes from Melia azedarach. Eur. J. Pharmacol. 406: 301-309, 2000.   DOI   ScienceOn
3 Chang, C.W., Lin, M.T., Lee, S.S., Liu, K.C., Hsu, F.L., Lin, J.Y. Differential inhibition of reverse transcriptase and cellular DNA polymerase-alpha activities by lignans isolated from Chinese herbs, Phyllanthus myrtifolius Moon, and tannins from Lonicera japonica Thunb and Castanopsis hystrix. Antiviral Res. 27(4):367-374, 1995.   DOI   ScienceOn
4 Lee, H.S., Kim, S.K., Han, J.B., Choi, H.M., Park, J.H., Kim, E.C., Choi, M.S., An, H.J., Um, J.Y., Kim, H.M., Min, B.I. Inhibitory effects of Rumex japonicus Houtt. on the development of atopic dermatitis-like skin lesions in NC/Nga mice. Br J Dermatol. 155(1):33-38, 2006.   DOI   ScienceOn
5 Paige, D.G., Morse-Fisher, N., Harper, J.I. Quantification of stratum corneum ceramides and lipid envelope ceramide in the hereditary ichthyoses. Br. J. dermatol. 131: 23-27, 1994.   DOI   ScienceOn
6 顧伯華. 實用中醫外科學. 上海科學技術出版社, 上海, pp 454-459, 461-464, 1985.
7 Aktan, F., Henness, S., Roufogalis, B.D., Ammit, A.J. Gypenosides derived from Gynostemma pentaphyllum suppress NO synthesis in murine macrophages by inhibiting iNOS enzymatic activity and attenuating NF-kappaB-mediated iNOS protein expression. Nitric Oxide. 8(4):235-242, 2003.   DOI   ScienceOn
8 Hibbs JB Jr, Taintor, R.R., Vavrin, Z. Macrophage cytotoxicity: role for L-arginine deiminase and imino nitrogen oxidation to nitrite. Science. 235(4787):473-476, 1987.   DOI
9 Seo, W.G., Pae, H.O., Oh, G.S., Chai, K.Y., Kwon, T.O., Yun, Y.G., Kim, N.Y., Chung, H.T. Inhibitory effects of methanol extracts of Cyrerus rotundus rhizomes on nitric oxide and superoxide production by murine macrophages cell line, RAW 264.7 cells. J Ethnopharmacol. 76: 59-64, 2001.   DOI   ScienceOn
10 변성희, 양재하, 김상찬. 현삼메탄올 추출물이 LPS로 유도된 Raw 264.7 cell에서의 $TNF-\alpha$, $IL-1\beta$, IL-6 및 nitric oxide 생성에 미치는 영향. 대한본초학회지 20(2):7-16, 2005.   과학기술학회마을
11 楊醫亞. 中醫學問答. 人民衛生出版社, 北京, pp 396-397, 1985.
12 楊思樹, 張樹生, 傅景華. 中醫臨床大全. 北京科學技術出版社, 北京, pp 888-890, 1991.
13 陳再民. 原說韓方醫藥大辭典(中國藥學大典). 中國, 講談社, p 160, 1982.
14 Lim, D.G., Sweeney, S., Bloodsworth, A., White, C.R., Chumley, P.H., Krishna, N.R., Schopfer, F., O'Donnell, V.B., Eiserich, J.P. Freeman BA. Nitrolinoleate, a nitric oxide-derived mediator of cell function: synthesis, characterization, and vasomotor activity. Proc Natl Acad Sci USA. 99(25):15941-15946, 2002.   DOI   ScienceOn
15 Aktan, F. iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-653, 2004.   DOI   ScienceOn
16 박민철, 김진만, 홍철희, 황충연. 아토피피부염의 동서의학적 문헌 고찰. 대한안이비인후피부과학회지 15(1):226-252, 2002.   과학기술학회마을
17 Uwe, S. Anti-inflammatory interventions of NF-kappaB signaling: potential applications and risks. Biochem Pharmacol. 75(8):1567-1579, 2008.   DOI   ScienceOn
18 국민건강보험공단 건강보험연구원. 2002-2007 환경성 질환 진료환자 분석. 2007.
19 이현희. 아토피피부염과 피부장벽. 소아알레르기 호흡기, 19(2):95-105, 2009.
20 Connelly, L., Palacios-Callender, M., Ameixa, C., Moncada, S., Hobbs, A.J. Biphasic regulation of NF-kappa B activity underlies the pro- and anti-inflammatory actions of nitric oxide. J Immunol. 166(6):3873-3881, 2001.   DOI
21 Hunnum, Y.A., Bell, R.M. Function of sphingolipid breakdown production in cellular regulation. Science. 243: 500-507, 1989.   DOI
22 Christophers, E., Mrowietz, U. The inflammatory infiltrate in psoriasis. Clin. Dermatol. 13: 131-135, 1995.   DOI   ScienceOn
23 Minehiro, O., Takashi, Y., Hideoki, O. Detergent-induced epidermal barrier dysfunction and its prevention. J. Dermatol. Sci. 30: 173-179, 2002.   DOI   ScienceOn
24 Halbert, A.R., Weston, W.L., Morelli, J.G. Atopic dermatitis: is it an allergic disease? J Am Acad Dermatol. 33(6):1008-1018, 1995.   DOI   ScienceOn
25 Chung, K.C., Kwon, D.Y., Baek, S.H., Kim, S.H., Chang, H.W. Effect of Lonicera Flos's ethyl acetate fraction on mutagenicity. Yakhak Hoeji. 32: 328-333, 1988.
26 Chang, H.W., Baek, S.H., Chung, K.W., Son, K.H., Kim, H.P., Kang, S.S. Inactivation of phospholipase A2 by naturally occurring biflavonoid, ochnaflavone. Biochem Biophys Res Commun. 205(1):843-849, 1994.   DOI   ScienceOn
27 Min-Hsiung Pan, Ching-Shu Lai, Ying-Jan Wang, Chi-Tang Ho. Acacetin suppressed LPS-induced up-expression of iNOS and COX-2 in murine macrophages and TRA-induced tumor promotion in mice. Biochem. Pharmacol. 72: 1293-1303, 2006.   DOI   ScienceOn
28 Min-Hsiung Pan, Shoei-Yn Lin-shiau, Jen-Kun Lin. Comparative Studies on the suppression of nitric oxide synthase by curcumin and Its Hydrogenated metabolites through down-regulation of $\kappa$ B kinase and NF-$\kappa$ B activation in macrophages. Biochem. Pharmacol. 60: 1665-1676, 2000.   DOI   ScienceOn
29 Ai-Hsiang LO, Yu-Chih Liang, Shoei-Yn Lin-Shiau, Chi-Tang Ho, Jen-Kun Lin. Carnosol, an antioxidant in rosemary, suppresses inducible nitric oxide synthase through down-regulating NF-$\kappa$ B in mouse macrophages. Carcinogenesis. 23(6):983-991, 2002.   DOI   ScienceOn
30 Choi, C.W., Jung, H.A., Kang, S.S., Choi, J.S. Antioxidant constituents and a new triterpenoid glycoside from Flos Lonicerae. Arch Pharm Res. 30(1):1-7, 2007.   과학기술학회마을   DOI   ScienceOn
31 Suh, S.J., Chung, T.W., Son, M.J., Kim, S.H., Moon, T.C., Son, K.H., Kim, H.P., Chang, H.W., Kim, C.H. The naturally occurring biflavonoid, ochnaflavone, inhibits LPS-induced iNOS expression, which is mediated by ERK1/2 via NF-kappaB regulation, in RAW264.7 cells. Arch Biochem Biophys. 447(2):136-146, 2006.   DOI   ScienceOn
32 Lowenstein, C.J., Alley, E.W., Raval, P., Snowman, A.M., Snyder, S.H., Russell, S.W., Murphy, W.J. Macrophage nitric oxide synthase gene: two upstream regions mediate induction by interferon gamma and lipopolysaccharide. Proc Natl Acad Sci USA. 90(20):9730-9734, 1993.   DOI   ScienceOn
33 Eiserich, J.P., Baldus, S., Brennan, M.L., Ma, W., Zhang, C., Tousson, A., Castro, L., Lusis, A.J., Nauseef, W.M., White, C.R., Freeman, B.A. Myeloperoxidase, a leukocyte-derived vascular NO oxidase. Science. 296(5577):2391-2394, 2002.   DOI   ScienceOn
34 Seo, W.G., Pae, H.O., Oh, G.S., Chai, K.Y., Yun, Y.G., Kwon, T.O., Chung, H.T. Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon and lipopolysaccharide. Gen Pharmacol. 35: 21-28, 2000.   DOI   ScienceOn
35 Lee, J.H., Ko, W.S., Kim, Y.H., Kang, H.S., Kim, H.D., Choi, B.T. Anti-inflammatory effect of the aqueous extract from Lonicera japonica flower is related to inhibition of NF-kappaB activation through reducing I-kappaBalpha degradation in rat liver. Int J. Mol Med. 7(1):79-83, 2001.
36 전국한의과대학 공동교재편찬위원회. 본초학. 서울, 永林社, pp 198-199, 2004.
37 보건복지가족부. 2005 국민건강영양조사. 2005.
38 배지현, 김미순, 강은혜. 식중독 유발세균의 증식에 미치는 금은화 추출물의 항균효과. 한국식품과학회지 37(4):642-647, 2005.   과학기술학회마을
39 김태윤. 아토피피부염 치료의 최신 경향. 소아알레르기 호흡기, 19(3):209-219, 2009.
40 나영호, 최선희. 아토피 피부염의 발병과 관련된 위험인자. 소아알레르기 호흡기, 19(2):91-94, 2009.