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Codonopsis Lanceolata Inhibits Inflammation through Regulation of MAPK in LPS-stimulated RAW264.7 cells  

Kim, Bum-Hoi (Department of Anatomy, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University)
Lee, Yong-Tae (Department of Oriental Physiology, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University)
Kang, Kyung-Hwa (Department of Oriental Physiology, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.1, 2010 , pp. 80-84 More about this Journal
Abstract
Codonopsis Lanceolata (CL) has been widely used in Oriental medicine for treatment of chronic inflammatory diseases, such as bronchitis, cough, and spasm; however, the mechanism of its anti-inflammatory activity has not been clarified. In this study, therefore, we investigated the inhibitory effect of CL on LPS-induced inflammation. The effect of CL was analyzed by ELISA, RT-PCR and Western blotting in LPS-stimulated RAW264.7 cells. We found that CL suppressed not only the mRNA expression of pre-inflammatory cytokines, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2, but also the phosphorylation of ERK1/2, JNK1/2 and p38 MAPK. These results suggest that CL exerts an anti-inflammatory effect through the regulation of the mitogen-activated protein kinases (MAPK) pathway, thereby decreasing production of pre-inflammatory cytokines, NO, and PGE2.
Keywords
codonopsis anceolata (CL); inflammation; mitogen-activated protein kinases (MAPK); RAW264.7;
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