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Effect of Myricetin in Osteoclast Differentiation and Bone Resorption  

Lee, An-Saeng (Wonkwang University Hospital)
Jang, Sung-Jo (Department of Neurosurgery, School of Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.1, 2010 , pp. 74-79 More about this Journal
Abstract
Osteoclasts are bone-resorbing giant cells that differentiate from hematopoietic cells of the monocyte/macrophages. Excessive osteoclast differentiation leads to gradual loss of bone mass causing fracture of the skeleton. The aim of this study was to develop a drug candidates for the treatment of osteoporosis. RANKL-induced osteoclast differentiation was dose-dependently inhibited by myricetin. Myricetin inhibited the expression of c-Fos, NFATc1, and TRAP in BMMs treated with RANKL. Myricetin disrupted the structure of actin ring and suppressed osteoclastic bone resorption. Also, myricetin induced apoptosis in mature osteoclasts. Myricetin inhibited the phosphorylation of ERK in mature osteoclasts treated with M-CSF. The activation of caspase-9 and caspase-3 was increased by myricetin treatment. Our results suggest that myricetin may be an effective agent to prevent bone diseases such as osteoporosis.
Keywords
bone; osteoclast; myricetin;
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1 Takayanagi, H., Kim, S., Koga, T., Nishina, H., Isshiki, M., Yoshida, H., Saiura, A., Isobe, M., Yokochi, T., Inoue, J., Wagner, E.F., Mak, T.W., Kodama, T., Taniguchi, T. Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev. Cell. 3: 889-901, 2002.   DOI   ScienceOn
2 Budihardjo, I., Oliver, H., Lutter, M., Luo, X., Wang, X. Biochemical pathways of caspase activation during apoptosis. Annu. Rev. Cell Dev. Biol. 15: 269-290, 1999.   DOI   ScienceOn
3 Takayanagi, H., Sato, K., Takaoka, A., Taniguchi, T. Interplay between interferon and other cytokine systems in bone metabolism. Immunol. Rev. 208: 181-193, 2005.   DOI   ScienceOn
4 Boyle, W.J., Simonet, W.S., Lacey, D.L. Osteoclast differentiation and activation. Nature. 423: 337-342, 2003.   DOI   ScienceOn
5 Sellappan, S., Akoh, C.C. Flavonoids and antioxidant capacity of Georgia-grown Vidalia onions. J. Agric. Food Chem. 50: 5338-5342, 2002.   DOI   ScienceOn
6 Waterhouse, A.L. Wine phenolics. Ann. N.Y. Acad. Sci. 957: 21-36, 2002.   DOI   ScienceOn
7 Wattel, A., Kamel, S., Prouillet, C., Petit, J.P., Lorget, F., Offord, E., Brazier, M. Flavonoid quercetin decreases osteoclastic differentiation induced by RANKL via a mechanism involving NF kappa B and AP-1. J. Cell Biochem. 92: 285-295, 2004.   DOI   ScienceOn
8 Jung, Y.T., Choi, Y.H., Song, J.H., Lee, C.H., Lee, M.S., Jang, S.J., Cho, H.J., Kwak, H.B., Oh, J. Effect of Water Extract of Eucommia ulmoides in RANKL-induced osteoclast differentiation. Korean J. Oriental Physiology & Pathology. 23: 613-618, 2009.   과학기술학회마을
9 Koga, T., Inui, M., Inoue, K., Kim, S., Suematsu, A., Kobayashi, E., Iwata, T., Ohnishi, H., Matozaki, T., Kodama, T., Taniguchi, T., Takayanagi, H., Takai T. Costimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasis. Nature. 428: 758-763, 2004.   DOI   ScienceOn
10 Lee, Z.H., Kim, H.H. Signal transduction by receptor activator of nuclear factor kappa B in osteoclasts. Biochem.Biophys. Res. Commun. 305: 211-214, 2003.   DOI   ScienceOn
11 Karsenty, G., Wagner, E.F. Reaching a genetic and molecular understanding of skeletal development. Dev. Cell. 4: 389-406, 2002.
12 Akiyama, T., Bouillet, P., Miyazaki, T., Kadono, Y., Chikuda, H., Chung, U.I., Fukuda, A., Hikita, A., Seto, H., Okada, T., Inaba, T., Sanjay, A., Baron, R., Kawaguchi, H., Oda, H., Nakamura, K., Strasser, A., Tanaka, S. Regulation of osteoclast apoptosis by ubiquitylation of proapoptotic BH3-only Bcl-2 family member Bim. EMBO J. 22: 6653-6664, 2003.   DOI   ScienceOn
13 Plas, D.R., Talapatra, S., Edinger, A.L., Rathmell, J.C., Thompson, C.B. Akt and Bcl-xL promote growth factor-independent survival through distinct effects on mitochondrial physiology. J. Biol. Chem. 276: 12041-12048, 2001.   DOI   ScienceOn
14 Lee, S.E., Chung, W.J., Kwak, H.B., Chung, C.H., Kwack, K.B., Lee, Z.H., Kim, H.H. Tumor necrosis factor-alpha supports the survival of osteoclasts through the activation of Akt and ERK. J. Biol. Chem. 276: 49343-49349, 2001.   DOI   ScienceOn
15 Suda, T., Takahashi, N., Udagawa, N., Jimi, E., Gillespie, M.T., Martin, T.J. Modulation of osteoclast differentiation and functionby the new members of the tumor necrosis factor receptor and ligand families. Endocr. Rev. 20: 345-357, 1999.   DOI   ScienceOn
16 Delaisse, J.M., Andersen, T.L., Engsig, M.T., Henriksen, K., Troen, T., Blavier, L. Matrix metalloproteinases (MMP) and cathepsin K contribute differently to osteoclastic activities. Microsc. Res. Tech. 61: 504-513, 2003.   DOI   ScienceOn
17 Zhao, H., Ross F.P. Mechanisms of osteoclastic secretion. Ann. N. Y. Acad. Sci. 1116: 238-244, 2007.   DOI   ScienceOn
18 Teitelbaum, S.L., Ross, F.P. Genetic regulation of osteoclast development and funtion. Nat. Rev. Genet. 4: 638-649, 2003.   DOI   ScienceOn
19 Matsuo, K., Galson, D.L., Zhao, C., Peng, L., Laplace, C., Wang, K.Z., Bachler, M.A., Amano, H., Aburatani, H., Ishikawa, H., Wagner, E.F. Nuclear factor of activated T-cells (NFAT) rescues osteoclastogenesis in precursors lacking c-Fos. J. Biol. Chem. 279: 26475-26480, 2004.   DOI   ScienceOn
20 Ross, F.P., Teitelbaum, S.L. alphavbeta3 and macrophage colony-stimulating factor: partners in osteoclast biology. Immunol. Rev. 208: 88-105, 2005.   DOI   ScienceOn
21 Fleischmann, A., Hafezi, F., Elliott, C., Reme, C.E., Ruther, U., Wagne,r E.F. Fra-1 replaces c-Fos-dependent functions in mice. Genes Dev. 14: 2695-2700, 2000.   DOI   ScienceOn
22 Takayanagi, H. Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems. Nat. Rev. Immunol. 4: 292-304, 2007.
23 Tsuji, M., Yamamoto, H., Sato, T., Mizuha, Y., Kawai, Y., Taketani, Y., Kato, S., Terao, J., Inakuma, T., Takeda, E. Dietary quercetin inhibits bone loss without effect on the uterus in ovariectomized mice. J. Bone Miner. Metab. 27: 673-681, 2009.   DOI   ScienceOn
24 Wakeyama, H., Akiyama, T., Kadono, Y., Nakamura, M., Oshima, Y., Nakamura, K., Tanaka, S. Posttranslational regulation of Bim by caspase-3. Ann. N.Y. Acad. Sci. 1116: 271-280, 2007.   DOI   ScienceOn
25 Yamaguchi, M., Hamamoto, R., Uchiyama, S., Ishiyama, K. Effect of flavonoid on calcium content in femoral tissue culture and parathyroid hormone-stimulated osteoclastogenesis in bone marrow culture in vitro. Mol. Cell Biochem. 303: 83-88, 2007.   DOI
26 Chang, R.L., Huang, M.T., Wood, A.W., Wong, C.Q., Newmark, H.L., Yagi, H., Sayer, J.M., Jerina, D.M., Conney, A.H. Effect of ellagic acid and hydroxylated flavonoids on the tumorigenicity of benzo[a]pyrene and (+/-)-7 beta, 8 alphadihydroxy- 9 alpha, 10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene on mouse skin and in the newborn mouse. Carcinogenesis. 6: 1127-1133, 1985.   DOI   ScienceOn
27 Hakkinen, S.H., Karenlampi, S.O., Heinonen, I.M., Mykkanen, H.M., Torronen, A.R. Content of the flavonols quercetin, myricetin, and kaempferol in 25 edible berries. J. Agric. Food Chem. 47: 2274-2279, 1999.   DOI   ScienceOn
28 Cho, H.J., Choi, M.K., Le, Y., Song, J.H., Lee, M.S., Lee, C.H., Jang, S.J., Kwak, H.B., Oh, J. Effect of water extracts of Cuscuta japonica Chois in RANKL-induced osteoclast differentiation. Korean J. Oriental Physiology & Pathology. 23: 860-865, 2009.   과학기술학회마을
29 Kim, Y.K., Choi, Y.H., Song, J.H., Jang, S.J. Kim, H.J., Lee, C.H., Ahn, S.H., Lee, J.E., Kim, J.J., Choi, M.K. Inhibitory Effect of Deer Antler on Osteoclastic Bone Resorption. Korean J. Oriental Physiology & Pathology. 23: 613-618, 2009.   과학기술학회마을
30 German, J.B., Walzem, R.L. The health benefits of wine. Annu. Rev. Nutr. 20: 561-593, 2000.   DOI   ScienceOn