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Effect of Water Extracts of Cuscuta Japonica Chois in RANKL-induced Osteoclast Differentiation  

Cho, Hae-Joong (Department of Obstetrics and Gynecology, School of Medicine, Wonkwang University)
Choi, Min-Kyu (Department of Anatomy, School of Medicine, Wonkwang University)
Kim, Jeong-Joong (Department of Anatomy, School of Medicine, Wonkwang University)
Le, Yan (Department of Anatomy, School of Medicine, Wonkwang University)
Song, Jeong-Hoon (Department of Plastic Surgery, School of Medicine, Wonkwang University)
Lee, Myeung-Su (Department of Rheumatology, School of Medicine, Wonkwang University)
Lee, Chang-Hoon (Department of Rheumatology, School of Medicine, Wonkwang University)
Jang, Sung-Jo (Department of Neurosurgery, School of Medicine, Wonkwang University)
Kwak, Han-Bok (Department of Anatomy, School of Medicine, Wonkwang University)
Oh, Jae-Min (Department of Anatomy, School of Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.4, 2009 , pp. 860-865 More about this Journal
Abstract
Osteoclasts are bone-resorbing multinucleated cells derived from the monocyte/macrophage lineage. The differentiation of osteoclasts are regulated by osteoblastic cells expressed RANKL, which is the most critical molecule for osteoclast differentiation. In this study, we found that water extracts of cuscuta inhibited RANKL-mediated osteoclast differentiation by direct action on bone marrow macrophages (BMMs) without cytotoxicity. In BMMs, water extracts of cuscuta inhibited the mRNA expression of c-Fos, NFATc1, TRAP, and OSCAR. Also, the protein expression of c-Fos and NFATc1 was inhibited by water extracts of cuscuta treatement. Water extracts of cuscuta inhibited the phosphorylation of p38, ERK, and JNK in BMMs treated with RANKL. However, water extracts of cuscuta did not inhibit RANKL-induced I-${\kappa}B$ activation. Water extract of cuscuta failed to inhibit bone resorption by osteoclasts cultured on hydroxyapatite plates. These results suggest that cuscuta may be a promising drug for use against bone disorders such as osteoporosis and rheumatoid arthritis.
Keywords
cuscuta; osteoclast; RANKL; NFATc1;
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1 Wagner, E.F., Karsenty, G. Genentic control of skeletaldevelopment. Curr. Opin. Genet. Dev. 11: 527-532, 2001   DOI   ScienceOn
2 Galibert, L., Tometsko, M.E., Anderson, D.M., Cosman, D., Dougall, W.C. The involvement of multiple tumor necrosis factor receptor (TNFR)-associated factors in the signaling mechanisms of receptor activator of NF-$\kappa$B, a member of the TNFR superfamily. J. Biol. Chem. 273: 34120-34127, 1998   DOI   ScienceOn
3 Teitelbaum, S.L. Bone resorption by osteoclasts. Science. 289: 1504-1508, 2000   DOI   PUBMED   ScienceOn
4 Takayanagi, H. Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems. Nat. Rev. Immunol. 7: 292-304, 2007   DOI   PUBMED   ScienceOn
5 Tanaka, S., Miyazaki, T., Fukuda, A., Akiyama, T., Kadono, Y., Wakeyama, H., Kono, S, Hoshikawa, S., Nakamura, M., Ohshima, Y., Hikita, A., Nakamura, I., Nakamura, K. Molecular mechanism of the life and death of the osteoclast. Ann. N. Y. Acad. Sci. 1068: 180-186, 2006   DOI   PUBMED   ScienceOn
6 Sambrook, P., Cooper, C. Osteoporosis. Lancet. 367: 2010- 2018, 2006   DOI   ScienceOn
7 Mansky, K.C., Sankar, U., Han, J., Ostrowski, M.C. Microphthalmia transcription factor is a target of the p38 MAPK pathway in response to receptor activator of NF-kappa B ligand signaling. J. Biol. Chem. 277: 11077-11083, 2002   DOI   ScienceOn
8 Rajotte, D., Sadowski, H.B., Haman, A., Gopalbhai, K., Meloche, S., Liu, L., Krystal, G., Hoang, T. Contribution of both STAT and SRF/TCF to c-fos promoter activation by granulocyte-macrophage colony-stimulating factor. Blood. 88: 2906-2916, 1996   PUBMED
9 Takeda, S., Yoshizawa, T., Nagai, Y., Yamato, H., Fukumoto, S., Sekine, K. Stimulation of osteoclast formation by 1,25-dihydroxyvitamin D requires its binding to vitamin D receptor (VDR) in osteoblastic cells: studies using VDR knockout mice. Endocrinology. 140: 1005-1008, 1999   DOI   PUBMED   ScienceOn
10 Karsenty, G., Wagner, E.F. Reaching a genetic and molecular understanding of skeletal development. Dev. Cell. 4: 389-406, 2002   DOI   PUBMED   ScienceOn
11 Boyle, W.J., Simonet, W.S., Lacey, D.L. Osteoclastdifferentiation and activation. Nature. 423: 337-342, 2003   DOI   ScienceOn
12 Jimi, E., Aoki, K., Saito, H., D'Acquisto, F., May, M.J., Nakamura, I., Sudo, T., Kojima, T., Okamoto, F., Fukushima, H., Okabe, K., Ohya, K., Ghosh, S. Selective inhibition of NF-$\kappa$B blocks osteoclastogenesis and prevents inflammatory bone destruction in vivo. Nat. Med. 10: 617-624
13 Ishida, N., Hayashi, K., Hoshijima, M., Ogawa, T., Koga, S., Miyatake, Y., Kumegawa, M., Kimura, T., Takeya, T. Large scale gene expression analysis of osteoclastogenesis in vitro and elucidation of NFAT2 as a key regulator. J. Biol. Chem. 277: 41147-41156, 2002   DOI   ScienceOn
14 Miyaura, C., Inada, M., Matsumoto, C., Ohshiba, T., Uozumi, N., Shimizu, T., Ito, A. An essential role of cytosolic phospholipase A2alpha in prostaglandin E2-mediated bone resorption associated with inflammation. J. EXP. Med. 197: 1303-1310, 2003   DOI   PUBMED   ScienceOn
15 Takayanagi, H., Kim, S., Koga, T., Nishina, H., Isshiki, M., Yoshida, H., Saiura, A., Isobe, M., Yokochi, T., Inoue, J., Wagner, E.F., Mak, T.W., Kodama, T., Taniguchi, T. Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev. Cell. 3: 889-901, 2002   DOI   ScienceOn
16 Lee, S.E., Woo, K.M., Kim, S.Y., Kim, H.M., Kwack, K., Lee, Z.H., Kim, H.H. The phosphatidylinositol 3-kinase, p38, and extracellular signal-regulated kinase pathways are involved in osteoclast differentiation. Bone. 1: 71-77, 2002
17 Yang, H.M., Shin, H.K., Kang, Y.H., Kim, J.K. Cuscuta chinensis extract promotes osteoblast differentiation and mineralization in human osteoblast-like MG-63 cells. J. Med. Food. 12(1):85-92, 2009   DOI   ScienceOn
18 Dougall, W.C., Glaccum, M., Charrier, K., Rohrbach, K., Brasel, K., De Smedt, T., Daro, E., Smith, J., Tometsko, M.E., Maliszewski, C.R., Armstrong, A., Shen, V., Bain, S., Cosman, D., Anderson, D., Morrissey, P.J., Peschon, J.J., Schuh, J. RANK is essential for osteoclast and lymph node development. Genes Dev. 13: 2412-2424, 1999   DOI   ScienceOn
19 Matsuo, K., Galson, D.L., Zhao, C., Peng, L., Laplace, C., Wang, K.Z., Bachler, M.A., Amano, H., Aburatani, H., Ishikawa, H., Wagner, E.F. Nuclear factor of activated T-cell(NFAT) rescues osteoclastogenesis in precursors lacking c-fos. J. Biol. Chem. 279: 26475-26480, 2004   DOI   ScienceOn
20 Chang, E.J., Ha, J., Huang, H., Kim, H.J., Woo, J.H., Lee, Y., Lee, Z.H., Kim, J.H., Kim, H.H. The JNK-dependent CaMK pathway restrains the reversion of committed cells during osteoclast differentiation. J. Cell. Sci. 121: 2555-2564, 2008   DOI   ScienceOn
21 Wei, S., Kitaura, H., Zhou, P., Ross, F.P., Teitelbaum, S.L. IL-1 mediates TNF-induced osteoclastogenesis. J. Clin. Invest. 115: 282-290, 2005   DOI   PUBMED   ScienceOn
22 이재복, 이병렬. 토사자약침의 항암작용 및 면역효과에 대한 실험적 연구. 대한침구학회지 18(3):94-104, 2001
23 May, M.J., D'Acquisto, F., Madge, L.A., Glockner, J., Pober. J.S., Ghosh, S. Selective inhibition of NF-$\kappa$B activation by a peptide that blocks the interaction of NEMO with the I$\kappa$B kinase complex. Science. 289: 1550-1554   DOI
24 Darnay, B.G., Haridas, V., Ni, J., Moore, P.A., Aggarwal, B.B. Characterization of the intracellular domain of receptor activator of NF-kappaB (RANK). Interaction with tumor necrosis factor receptor-associated factors and activation of NF-kappab and c-Jun N-terminal kinase. J. Biol. Chem. 273(32):20551-20555, 1998   DOI   ScienceOn
25 Matsumoto, M., Sudo, T., Saito, T., Osada, H., Tsujimoto, M. Involvement of p38 mitogen-activated protein kinase signaling pathway in osteoclastogenesis mediated by receptor activator of NF-kappa B ligand (RANKL). J. Biol. Chem. 275: 31155-31161, 2000   DOI   ScienceOn
26 Asagiri, M., Sato, K., Usami, T., Ochi, S., Nishina, H., Yoshida, H. Autoamplification of NFATc1 expression determines its essential role in bone homeostasis. J. Exp. Med. 202: 1261-1269, 2005   DOI   ScienceOn