Browse > Article

Protective Effects of Dohongsamul-tang on 2-deoxy-D-glucose Induced Autophagic Cell Death in C6 Glial Cells  

Shin, Hak-Soo (Department of Third Medicine, Professional Graduate School of Oriental Medicine, WonkWang University)
Lee, Seung-Geun (Department of Internal Medicine, School of Oriental Medicine, WonkWang University)
Moon, Byung-Soon (Department of Third Medicine, Professional Graduate School of Oriental Medicine, WonkWang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.3, 2009 , pp. 581-589 More about this Journal
Abstract
The water extract of Dohongsamul-tang(DHSMT) has been traditionally used in treatment of ischemic heart and brain diseases in Oriental Medicine. However, little is known about the mechanism by which DHSMT protects C6 glial cells from glucose deprevation induced damages. Therefore, this study was designed to evaluate the protective effects of DHSMT on 2-deoxy-D-glucose induced autophagy of C6 glial cells. Autophagic phenotype is evaluated by fluorescence microscopy and flow cytometry with specific biological staining dyes, including monodansylcadaverine and acridine orange, as well as Western blot analysis with microtubule-associated protein 1 light chain 3(LC3) and Beclin-1. Treatment with 2-deoxy-D-glucose significantly resulted in a decrease of the viability of C6 glial cells and increase of the extracellular LDH release in a dose and time-dependent manner. However, pretreatment with DHSMT protected C6 glial cells from glucose deprivation with 2-deoxy-D-glucose. The author also observed the fact that autophagy phenotype occurred by 2-deoxy-D-glucose in C6 glial cells. Pretreatment with 3-MA, a pharmacological inhibitior of autophagy, abolished the formation of acidic vesicle organelle in C6 glial cells treated with 2-deoxy-D-glucose. However, pretreatment with DHSMT inhibited the formation of autophagic phenotypes, including formation of acidic vesicle organelle, and increase of the expression of LC-3 II Beclin-1 proteins in C6 glial cells treated with 2-deoxy-D-glucose. Taken together, these data suggest that DHSMT is able to protect C6 glial cells from glucose deprivation with marked inhibition of autophagy formation.
Keywords
Dohongsamul-tang(DHSMT); 2-deoxy-D-glucose(2-DG); autophagic cell death; autophagy; C6 glial cell;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 하지용. 四物湯 및 四君子湯이 Endotoxin으로 유발된 血栓症에 미치는 영향. 경희한의대논문집, 11(1):113-121, 1988
2 곽근신. 桃紅四物湯이 Endotoxin으로 유발된 血栓症에 미치는 영향. 동의병리학회지 5(1):67-76, 1990
3 조한진, 김성훈. 桃紅四物湯加減方의 抗癌 및 抗轉移 효과에 관한 연구. 대한동의병리학회지 13(1):76-90, 1999
4 Nixon, R.A., Cataldo, A.M., Mathews, P.M. The endosomal-lysosomal system of neurons in Alzheimer's disease pathogenesis: a review. Neurochem Res. 25(9-10): 1161-1172, 2000   DOI   ScienceOn
5 Stefanis, L., Larsen, K.E., Rideout, H.J., Sulzer, D., Greene, L.A. Expression of A53T mutant but not wild-type alpha-synuclein in PC12 cells induces alterations of the ubiquitin-dependent degradation system, loss of dopamine release, and autophagic cell death. J Neurosci. 21(24):9549-9560, 2001
6 Adhami, F., Liao, G., Morozov, Y.M., Schloemer, A., Schmithorst, V.J., Lorenz, J.N., Dunn, R.S., Vorhees, C.V., Wills-Karp, M., Degen, J.L., Davis, R.J., Mizushima, N., Rakic, P., Dardzinski, B.J., Holland, S.K., Sharp, F.R., Kuan, C.Y. Cerebral ischemia-hypoxia induces intravascular coagulation and autophagy. Am J Pathol. 169(2):533-583, 2006   DOI   ScienceOn
7 한종현, 김기영. 한방약리학. 서울, 醫聖堂, p 169, 364, 375, 377, 479, 493, 2004
8 전용철. 當歸가 MCAO 모델에 휜쥐에서 gliosis 억제에 미치는 영향. 원광대학교대학원, 2008
9 김도환. Nitric Oxide에 의해 유발된 C6 glial 細胞毒性에 대한 四物湯의 防禦效果. 서울, 대한한방내과학회지 21(4): 535-542, 2000
10 김경선. 川芎의 口腔投與및 藥鍼施術이 흰쥐 MCAO로 유발된 局所腦虛血에 對한 콜린성 神經保護效果硏究. 동신대학교대학원, 2004
11 이지현. Protective effects of Daebojungtang on zinc-induced apoptosis in C6 glial cells. 원광대학교 한의학전문대학원, 2000
12 박세욱. 桃紅四物湯이 急性 腦硬塞 患者의 末梢血液單核細胞에서 細胞活性物質 生成에 미치는 영향. 원광대학교 대학원, 2004
13 이재원, 신진봉, 문구, 이종덕, 원진희. 桃仁이 중추신경 재생 촉진에 미치는 영향. 동의생리병리학회지 21(1):145-152, 2007
14 조권일. 배양심근세포의 산화적 손상에 대한 四物湯의 방어효과. 동의생리병리학회지 18(5):1374-1381, 2002
15 Meijer, A.J., Codogno, P. Regulation and role of autophagy in mammalian cells. Int. J. Biochem. Cell Biol. 36(12):2445-2462, 2004   DOI   ScienceOn
16 김요한. 攝生飮이 C6 glial 세포의 NO생성에 미치는 영향. 대한한의학회지 21(4):84-92, 2000
17 이영구, 문병순. 右歸飮이 Zinc에 의한 신경교세포의 고사에 미치는 영향. 대한한의학회지 22(3):63-73, 2001
18 유봉선. 산화적 손상에 의해 유발된 심근세포 독성에 대한 桃紅四物湯의 방어효과. 동의생리병리학회지 18(5):1374-1381, 2004
19 尹用甲. 東醫方劑와 處方解說. 서울, 醫聖堂, pp 711-712, 2002
20 박종운. H2O2에 의한 培養心筋細胞枯死에 미치는 四物湯의 防禦效果. 대한한의학회지 22(4):58-68, 2001
21 陳偉, 路一平. 方劑學. 上海, 上海中醫學院出版社, pp 206-207, 1990   PUBMED
22 전병훈, 정우열. 實驗的 血栓症에 미치는 韓藥材의 抗血栓效果에 관한 연구. 대한동의병리학회지 10(1):72-78, 1996
23 강창수. 四物湯 煎湯液이 家兎의 血壓降下에 미치는 영향. 원광한의대논문집, 2: 145-158, 1984
24 나영훈. 炙甘草湯이 LPS와 PMA에 의해 손상된 C6 glial 세포에 미치는 영향. 서울, 대한한방내과학회지 21(3):467-476, 2000
25 Zhang, Y., Wu, Y., Cheng, Y., Zhao, Z., Tashiro, S., Onodera, S., Ikejima, T. Fas-mediated autophagy requires JNK activation in HeLa cells. Biochem Biophys Res Commun. 377(4):1205-1210, 2008   DOI   ScienceOn
26 유제원. Paeoniae radix decreases intrastriatal hemorrhage-induced neuronal cell death and increases cell proliferation in the dentate gyrus of streptozotocin-induced hyperglycemic Rats. 경희대학교대학원, 2004
27 Li, D.Q., Li, Y., Liu, Y., Bao, Y.M., Hu, B., An, L.J. Catalpol prevents the loss of CA1 hippocampal neurons and reduces working errors in gebils after ischemia-reperfusion injury. Toxicon. 46(8):845, 2005   DOI   ScienceOn
28 Reunanen, H., Nyk$\ddot{a}$nen, P. A cytochemical study on the effects of energy deprivation on autophagocytosis in Ehrlich ascites tumor cells. Histochemistry. 90(3):177-184, 1988   DOI   ScienceOn
29 吳謙. 醫宗金鑑. 北京, 人民衛生出版社, pp 1145-1169, 1996
30 DiPaola, R.S., Dvorzhinski, D., Thalasila, A., Garikapaty, V., Doram, D., May, M., Bray, K., Mathew, R., Beaudoin, B., Karp, C., Stein, M., Foran, D.J., White, E. Therapeutic starvation and autophagy in prostate cancer: a new paradigm for targeting metabolism in cancer therapy. Prostate Dec. 1; 68(16):1743-1752, 2008   DOI   ScienceOn
31 Levine, B., Klionsky, D.J. Development by self-digestion: molecular mechanisms and biological functions of autophagy. Dev Cell. 6(4):463-477, 2004   DOI   ScienceOn
32 Sugawara, T., Fujimura, M., Noshita, N., Kim, G.W., Saito, A., Hayashi, T., Narasimhan, P., Maier, C.M., Chan, P.H. Neuronal death/survival signaling pathways in cerebral ischemia. Neuro Rx. 1(1):17-25, 2004   DOI   ScienceOn
33 朱震亨. 新編 丹溪心法附餘. 서울, 大星文化社, p 67, 69, 515, 533, 541, 1982
34 남창규. 四物湯이 血管內皮細胞에 미치는 영향. 대한한방내과학회지 20(1):83-98, 1999