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Effect of the Ethanol Extract of Artemisiae Capillaris Herba on the Hyperpigmentation Induced by ${\alpha}$-MSH  

Shin, Ki-Don (Department of Herbology, College of Oriental Medicine, Sangji University)
Kim, Dae-Sung (Department of Herbal Resources. Professional Graduate School of Oriental Medicine, WonkWang University)
Lee, Jang-Cheon (Division of Pharmacology, School of Oriental Medicine, Pusan National University)
Mun, Yeun-Ja (Department of Anatomy, College of Oriental Medicine, WonkWang University)
Woo, Won-Hong (Department of Herbal Resources. Professional Graduate School of Oriental Medicine, WonkWang University)
Lee, Young-Cheal (Department of Herbology, College of Oriental Medicine, Sangji University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.3, 2009 , pp. 574-580 More about this Journal
Abstract
Melanogenesis is induced mainly by ultraviolet radiation of sunlight and ${\alpha}$-Melanocyte stimulation hormone (${\alpha}$-MSH) which binds to a specific G protein coupled receptor. ${\alpha}$-MSH and cAMP-elevating agents are known to melanin syntheisis and dendrite outgrowth. The purpose of this study was to investigate the mechanism of melanogenesis inhibition in B16/F10 cells by ethanol extract of Artemisiae Capillaris Herba. In the present study, ${\alpha}$-MSH led to a stimulation of melanin synthesis that appeared to result from an increased tyrosinase activity and melanin content. However, the ethanol extract of Artemisiae Capillaris Herba inhibited the ${\alpha}$-MSH-induced tyrosinase activity and melanin content. In control conditions, B16/F10 cells displayed a fibroblastic appearance while ${\alpha}$-MSH treatment promoted the emergence of small and numerous dendrites from the plasma membrane. The ethanol extract of Artemisiae Capillaris Herba abolished the ${\alpha}$-MSH-induced dendricity. Regarding protein levels of the melanogenic enzymes, the amounts of tyrosinase were increased after incubation with ${\alpha}$-MSH. The treatment of Artemisiae Capillaris Herba ethanol extract decreased the ${\alpha}$-MSH expression levels of tyrosinase. Based on these findings, it is likely that the ethanol extract of Artemisiae Capillaris Herba exerts its depigmenting effects in B16/F10 cells through the suppression of tyrosinase expression, which are key enzymes for melanogenesis.
Keywords
Artemisiae Capillarisa; ${\alpha}$-MSH; melanin; tyrosinase; depigmentation; TRP-1;
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Times Cited By KSCI : 7  (Citation Analysis)
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