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Effect of Soyangin-Hyeongbangpaedok-san on Anti-CD3 Stimulated Mouse T Cells In Vivo and In Vitro  

Kim, Joong-Bae (Department of Pathology, College of Oriental Medicine & Institute of Oriental Medicine, Kyunghee University)
Kang, Hee (Department of East-West Medical Science, Graudate School of East-West Medical Science, Kyunghee University)
Ahn, Kwang-Seok (Department of Pathology, College of Oriental Medicine & Institute of Oriental Medicine, Kyunghee University)
Shim, Bum-Sang (Department of Pathology, College of Oriental Medicine & Institute of Oriental Medicine, Kyunghee University)
Kim, Sung-Hoon (Cancer Preventive Material Development Research Center, Kyunghee University)
Choi, Seung-Hoon (Department of Pathology, College of Oriental Medicine & Institute of Oriental Medicine, Kyunghee University)
Ahn, Kyoo-Seok (Department of Pathology, College of Oriental Medicine & Institute of Oriental Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.3, 2009 , pp. 554-561 More about this Journal
Abstract
Soyangin-Hyeongbangpaedok-san(SHBPDS) is a herbal formula used for the common cold or upper respiratory illness. In order to investigate the effect of SHBPDS, mice were orally administered with SHBPDS alcohol extract for 7 days followed by intravenous anti-CD3 injection. In addition, splenocytes and CD4 T cells were cultured with SHBPDS in response to anti-CD3 in vitro and cytokines and transcription factors were evaluated. In vivo treatment with SHBPDS significantly augmented the expressions of the percentage of CD4 T cells and CD 69, an indicator of early T cell activation. Serum levels of IL-4 were significantly increased but those of IFN-${\gamma}$ and IL-2 did not reach statistical significance. The expressions of IFN-${\gamma}$ and T-bet mRNA were significantly downregulated in SHBPDS treated mice while those of IL-4 and C-Maf were significantly upregulated. In vitro stimulation of splenocytes and CD4 T cells by SHBPDS resulted in a reduction in IFN-${\gamma}$ secretion and STAT4 activity. The IL-4 releases from both cells were slightly reduced, but STAT6 activity was rather increased. In conclusion, SHBPDS exerted an inhibition in the expression of IFN-${\gamma}$, T-bet and STAT4 while IL-4, C-Maf and STAT6 were increased. Further studies are required to examine its pharmacological effects using more appropriate animal experiments.
Keywords
Soyangin-Hyeongbangpaedok-san; IFN-gamma; IL-4; Th cell;
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