Browse > Article

Anti-oxidative and Anti-inflammatory Effects of Protulaca Oleracea on the LPS-stimulated AGS Cells  

Kim, Chae-Hyun (Department of Prescription, College of Oriental Medicine, Dongshin University)
Park, Pyeong-Beom (Department of Pathology, College of Oriental Medicine, Woosuk University)
Choe, Seung-Ryeol (Department of Pathology, College of Oriental Medicine, Woosuk University)
Kim, Tae-Heon (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Jeong, Jong-Kil (Department of Prescription, College of Oriental Medicine, Dongshin University)
Lee, Kwang-Gyu (Department of Pathology, College of Oriental Medicine, Woosuk University)
Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Jeong, Han-Sol (Department of Pathology, College of Oriental Medicine, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.2, 2009 , pp. 488-493 More about this Journal
Abstract
Protulaca oleracea, a widely distributed weed, has been reported to exhibit different health promoting effects. The objective of this study was to evaluate the anti-oxidative and anti-inflammatory effects of P. oleracea on LPS-stimulated AGS cells. The cytotoxicity of P. oleracea in AGS cells was examined by MTT assay. The anti-oxidative effects of P. oleracea were examined by DPPH assay. RT-PCR was carried out to examine the effect of P. oleracea in the mRNA expression of different inflammatory mediators. MTT assay revealed that P. oleracea have almost no cytotoxity in AGS cells. DPPH radical scavenging activities were better than butylated hydroxyl toluene (BHT). The mRNA expression of different endogenous anti-oxidative enzymes (SOD2, GPx3 and catalase) were preserved by P. oleracea in AGS cells. The nitric oxide production and expression of iNOS in LPS stimulated RAW264.7 were suppressed in P. oleracea treated groups. Based on these findings, P. oleracea has protective anti-oxidant and anti-inflammatory effects.
Keywords
portulaca oleracea(p. oleracea); SOD(superoxide dismutase); GSH(glutathione); ROS(reactive oxygen species); iNOS(inducible nitric oxide synthase);
Citations & Related Records
연도 인용수 순위
  • Reference
1 Chen, J., Shi, Y.P., Liu, J.Y. Determination of noradrenaline and dopamine herbal extracts from Portulaca oleracea L. by high-performance lipid chromatography. Journal of chromatography A pp 127-132, 2003   PUBMED
2 Park, J.S., Kim, K.U., Lee, Y.D. Physio-Ecological Characteristics of Portulaca oleracea L. Korean Journal of Weed Science 8: 23-27, 1988
3 Liu, L., Howe, P., Zhou, Y.F., Xu, Z.Q., Hocart, C., Zhang, R. Fatty acids and β-carotene in Australian purslane (Portulaca oleracea) varieties. Journal of Chromatography A pp 207-213, 2000   PUBMED
4 Sin, M.K. Clinical Traditional Herbalogy, Seoul. Younglimsa pp 417-418, 2002
5 Zheng, H.Z., Hong, D.Z., She, J. Modern study of Traditional Chinese Medicine,Beijing, Xue Yuan Press pp 753-761, 1997
6 Sakai, N., Inada, K., Okamoto, M., Shizuri, Y., Fukuyama, Y. Portuloside A, a monoterpene glucoside from Portulaca Oleracea. Phytochemistry 42: 1625-1628, 1996   DOI   ScienceOn
7 Fridovich, I. Biological effects of superoxide radical. Arch Biochem Biophy 247: 1-11, 1986   DOI   PUBMED   ScienceOn
8 Garti, N., Slavin, Y., Aserin, A. Surface and emulsification properties of a new gum extracted from Portulaca oleracea L. Food Hydrocolloids 13: 145-155, 1999   DOI   ScienceOn
9 Baeuerle, P.A., Baltimore, D. NF-$\kappa{B}$: Ten years after. Cell 87: 13, 1996   DOI   PUBMED   ScienceOn
10 Habtemariam, S., Harvey, A.L., Waterman, P.G. The muscle relaxant properties of Portulaca oleracea are associated with high concentrations of potassium ions. Journal of Ethnopharmacology 40: 195-200, 1993   DOI   ScienceOn
11 Rashed, A.N., Afifi, F.U., Disi, A.M. Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1. J. Ethnopharmacol. 88: 131-136, 2003   DOI   PUBMED   ScienceOn
12 G. Villarreal, J.Z., Wahl, S.M. Inflammation: Acute. Encyclopedia of Life Sciences pp 1-8, 2001
13 Mondal, S.K., Chakraborty, G., Gupta, M., Mazumder, U.K. In vitro antioxidant activity of Diospyros malabarica Kostel bark. Indian Journal of Experimental Biology 44: 39-44, 2006   PUBMED
14 Chan, K., Islam, M.W., Kamil, M., Radhakrishnan, R., Zakaria, M.N.M., Habibullah, M., Attas, A. The analgesic and anti-inflammatory effects of Portulaca oleracea L. subsp. sativa (Haw.) Celak. J. Ethnopharmacol. 73: 445-451, 2000   DOI   ScienceOn
15 Sakai, N., Inada, K., Okamoto, M., Shizuri, Y., Fukuyama, Y. Portuloside A, a monoterpene glucoside from Portulaca oleracea. Phytochemistry 42: 1625-1628, 1996   DOI   ScienceOn
16 Pihan, G., Regillo, C., Szabo, S. Free radicals and lipid peroxidation in ethanol- and aspirin-induced gastric mucosal injury. Dig Dis Sci 32: 1395-1401, 1987   DOI   ScienceOn
17 Parry, O., Okwuasaba, F.K., Ejike, C. Skeletal muscle relaxant action of an aqueous extract of Portulaca oleracea in the rat. Journal of Ethnopharmacology 19: 247-253, 1987   DOI   ScienceOn
18 Parry, O., Marks, J.A., Okwuasaba, F. The skeletal muscle relaxant action of Portulaca oleracea: role of potassium ions. J. Ethnopharmacol. 40: 187-194, 1993   DOI   ScienceOn
19 Na, H.J., Lee, G., Oh, H.Y., Jeon, K.S., Kwon, H.J., Ha, K.S., Lee, H., Kwon, Y.G., Kim, Y.M. 4-O-Methylgallic acid suppresses inflammation-associated gene expression by inhibition of redox-based NF-kappa B activation. Int. Immunopharmacol. 6: 1597-1608, 2006   DOI   ScienceOn
20 Yazici, I., Turkan, I., Sekmen, A.H., Demiral, T. Salinity tolerance of lipid peroxidation and proline accumulation. Environmental and Experimental Botany pp 49-57, 2007
21 Singleton, V.L., Rossi, J.A. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. American Journal of Enology and Viticulture pp 144-158, 1965
22 Li, X., Wang, H. Chinese herbal medicine in the treatment of chronic kidney disease. Adv. Chronic Kidney Dis. 12: 276-281, 2005   DOI   ScienceOn
23 Tatemichi, M., Ogura, T., Nagata, H., Esumi, H. Enhanced expression of inducible nitric oxide synthase in chronic gastritis with intestinal metaplasia. J Clin Gastroenterol. 27(3):240-245, 1998   DOI   ScienceOn
24 Abas, F., Lajis, N.H., Israf, D.A., Khozirah, S., Kalsom, Y.U. Antioxidant and nitric oxide inhibition activities of selected Malay traditional vegetables. Food Chemistry pp 566-573, 2006   DOI   ScienceOn
25 Zhang, X.J., Ji, Y.B., Qu, Z.Y., Xia, J.C., Wang, L. Experimental studies on antibiotic functions of Portulaca oleracea L. in vitro. Chinese J. Microecol 14: 277-280, 2002
26 Isin, Y., Ismail, T., Askim, H.S., Tijien, D. Salinity tolerance of purslane (Portulaca oleracea L.) is achieved by enhanced antioxidative system, lower level of lipid peroxidation and proline accumulation. Environmental and Experimental Botany pp 49-57, 2007   DOI   ScienceOn
27 Mannick, E.E., Bravo, L.E., Zarama, G., Realpe, J.L., Zhang, X.J., Ruiz, B., Fontham, E.T.H., Mera, R., Miller, M.J.S., Correa, P. Inducible nitric oxide synthase, nitrotyrosine, and apoptosis in Helicobacter pylori gastritis: effect of antibiotics and antioxidants. Cancer Res. 56: 3238-3243, 1996   PUBMED
28 Awad, N.E. Lipid content and antimicrobial activity of phenolic constituents of cultivated Portulaca oleracea L. Bull. Fac. Pharm. Cairo Univ. 32: 137-142, 1994
29 Babcock, T.A., Helton, W.S., Hong, D., Espat, N.J. Omega-3 fatty acid lipid emulsion reduces LPS-stimulated macrophage TNF-$\alpha$ production. Surg Inf 3: 145-149, 2002   DOI   ScienceOn
30 Teng, J.L., Cui, H. Chinese Materia Medica, Beijing, People's Medical Publishing House p 65, 2007
31 Florian L.M., Michael S.L., Youngmok J., Arlan R. Holly V.R. Trends in oxidative aging theories. Free Radical Biology and Medicine 43(4):477-503, 2007   DOI   ScienceOn
32 Lim, Y.Y., Quah. Antioxidant properties of different cultivars of Portulaca oleracea. Food chemistry pp 734-740, 2007   DOI   ScienceOn
33 Ahn, D.K. Illustrated Book of Korean Medicinal Plant; kyo-hak publishing Co. p 66, 2003