Browse > Article

Effects of Seonpye-tang on OVA-Induced Asthmatic Mice  

Lee, Ju-Guan (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Park, Yang-Chun (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.2, 2009 , pp. 457-463 More about this Journal
Abstract
This study aimed to evaluate the anti-asthmatic effects of Seonpye-tang (SPT) using OVA-induced asthmatic mice model. Scavenging activity of SPT on DPPH free radical and SOD-like activity of SPT were measured at final concentration 62.5, 125, 250, 500 (${\mu}g/m{\ell}$), RBL-2H3 cells were treated with DNP IgE for 24hr, and treated with SPT (1, 10, 100 ${\mu}g/m{\ell}$) for 1hr, followed by treatment with DNP-HSA for 1hr at $37^{\circ}C$. The level of IL-4 and TNF-${\alpha}$ were measured by ELISA. Asthmatic mice model was conducted by repeated challenge of OVA using C57BL/6 mice. Each group was treated with distilled water, SPT (400 mg/kg and 200 mg/kg) extract or cyclosporin A (10 mg/kg) for the later 8 weeks. Immune cells subpopulation, eotaxin, IL-5 and TNF-${\alpha}$ in BALF were analyzed. SPT dose-dependently increased Scavenging activity on DPPH free radical and SOD-like activity. SPT significantly ameliorated the increase of total cells number and eosinophil including of immune cell subpopulation of $CD3^+/CD69^+$, $CCR3^+$, $B220^+/CD22^+$, $B220^+/CD45^+$ and $B220^+/IgE^+$ cells in BALF comparing to control group. Eotaxin and IL-5 level in BALF were significantly decreased by SPT. These results strongly suggest that SPT would be a effective candidate for herbal-originated anti-asthmatic drug. However, this drug should be further studied for characterization of the accurate action and underlying mechanisms using variant disease model in the future.
Keywords
Seonpye-tang; asthma; antioxidative effect; immune cell; cytokine;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 Masoli, M., Fabian, D., Holt, S., Beasley, R. The global burden of asthma: executive summary of the GINA Dissemination Committee report. Allergy 59(5):469-478, 2004   DOI   ScienceOn
2 국민건강보험공단. 최근 6년(2002~2007)간 황사관련 봄철 다발생 질환 건강보험 의료기관 이용자 분석. http://www.nhic.or.kr.
3 전국한의과대학 폐계내과학교실. 동의폐계내과학. 서울, 한문화사, pp 329-331, 2002
4 홍천수, 김규언. 기관지천식-병인 및 역학, 대한 천식 및 알레르기학회, 천식과 알레르기 질환. 서울, 군자출판사, pp 244-245, 2002
5 Holgate, S.T. Pathogenesis of asthma. Clin Exp Allergy 38(6):872-897, 2008   DOI   ScienceOn
6 허준. 동의보감. 서울, 남산당, p 480, 1986
7 송상진, 박양춘. 조각자가 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 대한한방내과학회지 26(1):143-155, 2005   과학기술학회마을
8 송재진, 박양춘. 반하가 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 대한한방내과학회지 26(1):156-168, 2005   과학기술학회마을
9 정승기, 이형구. 정천탕이 천식에 미치는 영향에 관한 실험적 연구. 경희의학 3(1):91-102, 1987
10 Bateman, E.D., Boushey, H.A., Bousquet, J., Busse, W.W., Clark, T.J., Pauwels, R.A., Pedersen, S.E. GOAL Investigators Group. Can guideline-defined asthma control be achieved? The Gaining Optimal Asthma ControL study. Am J Respir Crit Care Med. 170(8):836-844, 2004   DOI   ScienceOn
11 Sch$\ddot{a}$cke, H., D$\ddot{o}$cke, W.D., Asadullah, K. Mechanisms involved in the side effects of glucocorticoids. Pharmacol Ther. 96(1):23-43, 2002   DOI   ScienceOn
12 Booth, B.W., Newcomb, D.C., McKane, S.A., Crews, A.L., Adler, K.B., Bonner, J.C., Martin, L.D. Proliferation of the airway epithelium in asthma: are inflammatory cells required? Chest. 123(3 Suppl):384S-385S, 2003   DOI   PUBMED
13 Hartnel, A., Robinson, D.S., Kay, A.B., Wardlaw, A.J. CD69 is expressed by human eosinophils activated in vivo in asthma and in vitro by cytokines. Immunology. 80(2):281-286, 1993   PUBMED
14 Tedder, T.F., Tuscano, J., Sato, S., Kehrl, J.H. CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling. Annu Rev Immunol. 15: 481-504, 1997   DOI   ScienceOn
15 조상헌. IgE 항체 생성과 조절. 알레르기, 13: 460-464, 1993
16 Adcock, I.M., Caramori, G., Chung, K.F. New targets for drug development in asthma. Lancet. 372(9643):1073-1087, 2008   DOI   ScienceOn
17 이영우, 정희재, 이형구, 정승기. 정천탕과 청상보하탕이 asthma model 내의 cytokine에 미치는 영향. 대한한방내과학회지 22(3):367-377, 2001   과학기술학회마을
18 Sabroe, I., Conroy, D.M., Gerard, N.P., Li, Y., Collins, P.D., Post, T.W., Jose, P.J., Williams, T.J., Gerard, C.J., Ponath, P.D. Cloning and characterization of the guinea pig eosinophil eotaxin receptor, C-C chemokine receptor-3: blockade using a monoclonal antibody in vivo. J Immunol. 161(11):6139-6947, 1998
19 Combadiere, C., Ahuja, S.K., Murphy, P.M. Cloning and functional expression of a human eosinophil CC chemokine receptor.J Biol Chem. 270(28):16491-16494, 1995   DOI   ScienceOn
20 Gangur, V., Birmingham, N.P., Thanesvorakul, S., Joseph, S. CCR3 and CXCR3 as drug targets for allergy. principles and potential. Curr Drug Targets Inflamm Allergy. 2(1):53-62, 2004   DOI   ScienceOn
21 Brightling, C., Berry, M., Amrani, Y. Targeting TNF-alpha: a novel therapeutic approach for asthma. J Allergy Clin Immunol. 121(1):5-10, 2008   DOI   ScienceOn
22 Kirkham, P., Rahman, I. Oxidative stress in asthma and COPD: antioxidants as a therapeutic strategy. Pharmacol Ther. 111(2):476-494, 2006   DOI   ScienceOn
23 Related Articles, LinksKing, M.R., Ismail, A.S., Davis, L.S., Karp, D.R. Oxidative stress promotes polarization of human T cell differentiation toward a T helper 2 phenotype. J Immunol. 176(5):2765-2772, 2006   DOI
24 Gerberick, G.F., Cruse, L.W., Ryan, C.A., Hulette, B.C., Chaney, J.G., Skinner, R.A., Dearman, R.J., Kimber, I. Use of a B cell marker (B220) to discriminate between allergens and irritants in the local lymph node assay. Toxicol Sci. 68(2):420-428, 2002.   DOI   ScienceOn
25 Rabe, K.F., Adachi, M., Lai, C.K., Soriano, J.B., Vermeire, P.A., Weiss, K.B., Weiss, S.T. Worldwide severity and control of asthma in children and adults: the global asthma insights and reality surveys. J Allergy Clin Immunol. 114(1):40-47, 2004   DOI   ScienceOn
26 전국한의과대학 본초학교실. 本草學. 서울, 영림사, p 295, 370, 409, 506, 509, 1994
27 곽상교, 최선미, 박양춘. 관동화가 천식모델 생쥐의 BALF내 면역세포 및 사이토카인에 미치는 영향. 동의생리병리학회지 19(3):716-721, 2005   과학기술학회마을
28 Forssmann, U., Uguccioni, M., Loetscher, P., Dahinden, C.A., Langen, H., Thelen, M., Baggiolini, M. Eotaxin-2, a novel CC chemokine that is selective for the chemokine receptor CCR3, and acts like eotaxin on human eosinophil and basophil leukocytes. J Exp Med 185(12):2171-2176, 1997   DOI   ScienceOn
29 한영주, 박양춘. 감초(Glycyrrhiza uralensis Fisch, GLU)가 천식모델 생쥐의 BALF내 면역세포 및 Cytokine에 미치는 영향. 대한한방내과학회지 25(3):408-417, 2004   과학기술학회마을
30 이정은, 박양춘. 삼자화담전이 천식모델 생쥐의 면역세포인에 미치는 영향. 대한한방내과학회지 29(3):742-751, 2008   과학기술학회마을
31 Justement, L.B. The role of the protein tyrosine phosphatase CD45 in regulation of B lymphocyte activation. Int Rev Immunol. 20(6):713-738, 2001   DOI   PUBMED   ScienceOn
32 Dabbagh, K., Takeyama, K., Lee, H.M., Ueki, I.F., Lausier, J.A., Nadel, J.A. IL-4 induces mucin gene expression and goblet cell metaplasia in vitro and in vivo. J Immunol. 162(10):6233-6237, 1999
33 임지택, 박양춘. 자하거 약침이 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 동의생리병리학회지 19(2):408- 417, 2005   과학기술학회마을
34 조철준, 임도희, 황지호, 양수영, 박양춘. 황금이 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 대한한방내과학회지 27(1):114-125, 2006   과학기술학회마을
35 염종훈, 정희재, 정승기, 이형구. 정천탕과 정천탕가미방이 알레르기 천식모델 흰쥐의 BALF내 면역세포 및 혈청 IgE에 미치는 영향. 대한한의학회지 24(1):169-180, 2003   과학기술학회마을
36 Erin, E.M., Williams, T.J., Barnes, P.J., Hansel, T.T. Eotaxin receptor (CCR3) antagonism in asthma and allergic disease. Curr Drug Targets Inflamm Allergy. 1(2):201-214, 2002   DOI   ScienceOn
37 나도균, 박양춘. 가미지황탕이 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 대한한의학회지 29(2):7-20, 2008   과학기술학회마을
38 Son, C.G., Seol, I.C. The experimental study of Jungchun-tang on allergies. 대한한의학회지 24(3):65-71, 2003   과학기술학회마을
39 Chung, K.F., Barnes, P.J. Cytokines in asthma. Thorax. 54(9):825-857, 1999   DOI   ScienceOn