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Inhibitory Effect of Extract of Euonymus alatus (Thunb.) Sieb. on the Production of Inflammatory Cytokines  

Kim, Dae-Hyeon (Department. of Pharmacy, College of Oriental Pharmacy, Wonkwang University)
Kim, Byung-Jin (Department. of Pharmacy, College of Oriental Pharmacy, Wonkwang University)
Park, Kyung-Bae (Department. of Pharmacy, College of Oriental Pharmacy, Wonkwang University)
Lee, Ju-Sung (Department of Circulatory Internal Medicine, Wonkwang University)
Sung, Kang-Keyng (Department of Circulatory Internal Medicine, Wonkwang University)
Park, Sung-Joo (Department of Herbology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.2, 2009 , pp. 368-373 More about this Journal
Abstract
The purpose of this study was to investigate the anti-inflammatory effects of water extract from Euonymus alatus (Thunb.) Sieb. (EAS) on the RAW 264.7 cells. To evaluate of anti-inflammatory of EAS, we examined the cytokine productions on lipopolysaccharide (LPS)-induced RAW 264.7 cells and also inhibitory mechanisms using Western blot. EAS reduced LPS-induced production of nitric oxide (NO), interleukin (IL)-1b, IL-6, IL-10 and tumor necrosis factor-a (TNF-a) in RAW 264.7 cells. EAS inhibited the activation of mitogen-activated protein kinases (MAPKs) such as p38, extracelluar signal-regulated kinase (ERK 1/2) and c-Jun NH2-terminal kinase (JNK) but not of inhibitory kappa B a (Ik-Ba) degradation in the LPS-stimulated RAW 264.7 cells. In conclusion, EAS down-regulated LPS-induced NO and cytokines production, which could provide a clinical basis.
Keywords
Euonymus alatus (Thunb.) Sieb.(EAS); lipopolysaccharide (LPS); inflammation; cytokine;
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