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Effects of Lactobacillus Plantarum Cultured in Platycodi Radix Decoction on the Eexpression of NO and TNF-${\alpha}$ in Mouse Macrophage RAW264.7 Cell Line  

Kim, Seong-Won (Department of Pathology, College of Oriental Medicine. & Institute of Oriental Medicine, Kyunghee University)
Kang, Hee (Department of East-West Medical Science, Graduate School of East-West Medical Science)
Ahn, Kwang-Seok (Department of Pathology, College of Oriental Medicine. & Institute of Oriental Medicine, Kyunghee University)
Shim, Bum-Sang (Department of Pathology, College of Oriental Medicine. & Institute of Oriental Medicine, Kyunghee University)
Kim, Sung-Hoon (Cancer Preventive Material Development Research Center)
Choi, Seung-Hoon (Department of Pathology, College of Oriental Medicine. & Institute of Oriental Medicine, Kyunghee University)
Ahn, Kyoo-Seok (Department of Pathology, College of Oriental Medicine. & Institute of Oriental Medicine, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.2, 2009 , pp. 331-336 More about this Journal
Abstract
This study examined the effects of Lactobacillus plantarum(LP) cultured in Platycodi Radix decoction(LPPR) on the expressions of NO and TNF-${\alpha}$ in mouse macrophage RAW264.7 cell line. Cells were stimulated with LP or LPPR (0.1, 1, and 10 bacteria/cell) in all assays. More NO was induced in LPPR than LPM at 0.1 and 1 of a LP: cell ratio. The iNOS mRNA expression was also enhanced in LPPR stimulated cells. TNF-${\alpha}$ was increased in LPPR stimulated cells at the protein and mRNA level compared with LPM. In conclusion, LP fermented in Platycodi Radix decoction induced stronger activity in NO and TNF in mouse macrophages than LPM. These results suggest that fermentation by Platycodi Radix can be useful in enhancing the immunostimulatory activity of LP.
Keywords
platycodi radix; TNF-${\alpha}$; NO; lactobacillus plantarum(LP); fermentation;
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