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Sophora Flavescens Suppresses Degranulation and Pro-inflammatory Cytokines Production through the Inhibition of NF-${\kappa}B$ (p65) Activation in the RBL-2H3 cells  

Lyu, Ji-Hyo (Clinical Research Center of Oriental Medicine, Dongeui University)
Park, Sang-Eun (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Hong, Su-Hyun (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Kim, Dong-Kyu (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Ko, Woo-Shin (Clinical Research Center of Oriental Medicine, Dongeui University)
Hong, Sang-Hoon (Clinical Research Center of Oriental Medicine, Dongeui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.1, 2009 , pp. 206-213 More about this Journal
Abstract
Sophora flavescens, as a traditional herbal medicine, has been used to treat with a variety of disesases, In previous reports, S. flavescens and sophoraflavanone G (a prenylated flavonoid from S. flavescens) inhibited cytokines productions in LPS-induced Raw 264.7 macrophages cells and BV2 microglial cells. We examined on the anti-allergic effect of S. flavescens on the PMA plus A23187-induced rat leukemia (RBL-2H3) cells. S. flavescens inhibited the release of $\beta$-hexosaminidase and productions and expressions of tumor necrosis factor (TNF)-$\alpha$, interleukin (IL)-4 and cyclooxygenase (COX)-2 in a dose-dependent manner on stimulated RBL-2H3 cells, however, S. flavescens not affect cell viability. The protein expression level of nuclear factor (NF)-${\kappa}B$ (p65) was decreased in the nucleus and suppressed the degradation of inhibitory protein $I{\kappa}B-{\alpha}$ protein, the activation of extracellular signal-regulated kinases (ERK) mitogen-activated protein kinase (MAPK) by S. flavescens. These results suggest that S. flavescens could be involved anti-allergic effect by control of $NF-{\kappa}B$ (p65) translocation into the nucleus through inhibition of $I{\kappa}B-{\alpha}$ degradation and suppression of pro-inflammatory cytokines expression.
Keywords
Sophora flavescens; pro-inflammatory cytokines; nuclear factor kappa B; Inhibitor kappa B; ERK Mitogen-Activated protein kinase;
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