Browse > Article

Hepatoprotective Activity of Salidroside Isolated from Acer Termentosum Max on D-galactosamine Induced Hepatotoxicity in Rats  

Kim, Sung-Hoon (Oriental Medical College, Kyunghee University)
Park, Hee-Juhn (Department of Pharmaceutical Engineering, Sangji University)
Choi, Jon-Won (College of Pharmacy, Kyungsung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.6, 2008 , pp. 1525-1531 More about this Journal
Abstract
Acer tegmentosum Max which is one of the specialized wildness medicinal herbs in gangwon province, has been widely used for hepatitis, liver cirrhosis, hepatic cancer, leukemia, diabetes mellitus, renal necrosis and edema, etc. In this study, the antioxidative and hepatoprotective effects of in vitro and in vivo were investigated in order to evaluate the possibility as hepatoprotective agents. Oral administration of methanol and butanol extact of Acer tegmentosum Max to d-galactosamine (D-GaIN) induced experimental liver injured rats was significantly reduced activities of marker enzymes(AST, ALT) and LDH activity in serum. Salidroside(Sal) isolated from the BuOH extract of Acer termentosum Max potenty showed the scavenzing effect on DPPH and inhibitory effect on lipid peroxidation. And significantly decrcease of MDA level in liver and activities of SOD GSH-Px and catalase were significantly improved by the treatment of Sal. Results of this study revealed that Sal could afford a significant protection in the alleviation of D-GaIN-induced hepatocellular injury.
Keywords
Acer tegmentosum Max; salidroside; d-galactosamine; hepatoprotective effect;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Conti, M., Malandrino, S., Magistretti, M.J. Protective activity of silipide on liver damage in rodents. Japan. J. Pharmacol. 60: 315-321, 1992.   DOI
2 김부생, 최종원, 이정규, 천련자 추출물이 간기능에 미치는 영향 (1) - 천련자의 분획이 약물대사효소계 및 담즙분비에 미치는 영향, 생약학회지 24: 63-68, 1993.
3 이창복. 대한식물도감. 항문사, 서울, p 552, 1993.
4 소배근. 중국본초도감(3권), 여강출판사, 서울, p 193, 1994.
5 Jang, S.I., Pae, H.O., Choi, B.M., Oh, G.H., Jeong, S., Lee, H.J., Kim, H.Y., Kang, K.H., Yun, Y.G., Kim Y.C., Chung, H.T. Salidroside from Rhodiola sachalinesis protects neuronal PC 12 cells against cytotoxicity induced by amyloid-$\beta$, Immunopharmacol. Immunotoxicol. 25: 295-304, 2003.   DOI   ScienceOn
6 Wang, S.H., Wang, W.J., Wang, X.F., Chen, W.H. Effect of salidroside on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes. Zhong Xi He Xue Bao 2: 193-195, 2004.   DOI   ScienceOn
7 Wroblewskin, F., LaDue, J.S. Lactic dehydrogenase activity in blood. Proc. Soc. Exp. Biol. Med. 90: 210-213, 1955.
8 Luczaj, W., Skrzydlewska, E. The present-day look at lipid peroxidation. Postepy Biochem. 52(2):173-179, 2006.
9 Campos, R., Garrido, A., Guerra, R., Valenzuela, A. Acetaminophen hepatotoxicity in rats is attenuated by silybin dihemisuccinate. Progress in Clin. Biol.l Res. pp 375-280, 1988.
10 Reitman, S., Frankel, S. A colorimetric method for the detemination of serum Glutamic oxaloacetic acid and Glutamic pyruvic transaminase. Am. J. Clin Phathol. 28: 56-63, 1957.   DOI
11 Zhang, X.S., Zhu, B.D., Hung, X.Q., Chen, Y.F. Effect of salidroside on bone marrow cell cycle and expression of apoptosis-related proteins in bone marrow cells of bone marrow depressed anemia mice. Sichuan Da Xue Xue Bao Yi Xue Ban 36: 820-823, 2005.
12 Wilson, S.R., Zucker, P.A., Huang, R.R.C., Spector, A. Development of synthetic compounds with glutathione peroxidase activity. J. Am. Chem. Soc. 111: 5936-5939, 1989.   DOI
13 Dubin, M., Groszmann, R.J., Kravetz, D. Effect of silimarin on hepatotoxicity induced by carbon tetrachloride in rats. Medicina, 36: 437-442, 1976.
14 Decker, K., Keppler, D., Pausch, J. The regulation pyridine nucleotide level and its role in experimental hepatitis. Adv. Enzyme Regul. 11: 205-230, 1973.   DOI   ScienceOn
15 Vimal, V., Devaki, T. Hepatoprotective effect of allicin on tissue defense system in galactosamine/ endotoxin challenged rats. J. of Ethnopharm. 90: 151-154, 2004.   DOI   ScienceOn
16 Mertens, K., Rogiers, V., Vercruysse, A. Measurement of malondialdehyde in cultures of adult rat hepatocytes. Toxic. in Vitro, 7: 439-441, 1993.   DOI   ScienceOn
17 김안근, 김지현, 산화적 스트레스 및 항산화제가 항산화효소 활성에 미치는 영향. 응용약물학회지 9: 249-257, 2001.
18 Niki, E., Yoshida, Y., Saito, Y., Noguchi, N. Lipid peroxidation: mechanisms, inhibition, and biological effects. Biochem. Biophys. Res. Commun. 338(1):668-676, 2005.   DOI   ScienceOn
19 Cutteridge, J.M.C. Antioxidant properties of the proteins ceruloplasmin, albumin and transferrin. A study of their activity in serum and synocial fluid from patients with rheumatoid arthritis. Biochem. Biophys. Acta, 869: 119-127, 1986.   DOI   ScienceOn
20 Bartholomaeus, A., Ahokas, J. Inhibition of P-450 by aucubin: Is the biological activity of aucubin due to its glutaraldehyde-like aglycone. Toxicol. Letters, 80: 75-83, 1995.   DOI
21 Braughler, J.M., Chase, R.L., Pregenzer, J.F. Oxidation of ferrous iron during peroxidation of various lipid substance. Biochem. Biophys. Acta, 921: 457-467, 1987.   DOI   ScienceOn
22 Stefan, M., Gudrun, M. Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J Biochem., 47: 469-474, 1974.   DOI   ScienceOn
23 임화경, 김학성, 최종원. Bergenin 및 acetylbergenin의 사염화탄소 유발 간독성에 대한 치료효과 I. 응용약물학회지 8: 293-298, 2000.
24 Lowry, O.H., Rodebrough, N.J. Farr, A.L., Randall, R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275, 1951.
25 Spiteller, G. The important role of lipid peroxidation processes in aging and age dependent diseases. Mol. Biotechnol. 37(1):5-12, 2007.   DOI   ScienceOn
26 Keppler, D., Decker, K. Studies on the mechanism of galactosamine hepatitis; Accumulation of galactosamine I-phosphate and is inhibition of UDP-glucose pyrophosphorylase. Eur. J. Biochem, 10: 219-225, 1969.   DOI   ScienceOn
27 Yokozawa, T., Dong, E., Wu, L.Z., Oura, H. and Nishioka, I. Antioxidant activity of Wen-Pi-Tang in vitro. Natural Med. 50: 243-246, 1966.
28 Shindo, Y., Hashimoto, T. Time course of changes in antioxidant enzymes in human skin fibroblasts afer UV irradiation. J. Dermatol. Sci. 14: 225-232, 1997.   DOI   ScienceOn
29 Gregus, Z., Klaassen, C.D. Mechanisms of toxicity. In casarett and Doull's Toxicology. McgrawHill Com. New York. pp 39-43, 1996.
30 Zhang, Y., Liu, Y. Study on effect of salidroside on lipid peroxidation in oxidative stress in rat hepatic stellate cells. Zhong Yao Cai. 28: 794-796, 2005.
31 Terry, D.O., Janice, L,S., Ning, L., Larry, W.O. Antioxidant enzyme levels as a function of growth state in cell culture. Free Radical Bilo. Med. 19: 53-65, 1995.   DOI   ScienceOn
32 Blois, M.S. Antioxidant determinations by the use of a stable free radical. Nature, 181: 1199-1200, 1958.   DOI   ScienceOn
33 Yosjikawa, T., Murakami, M., Yoshida, N., Seto, O., Kondo, M. Effects of superoxide dimutase and catalase on disseminated intravascular coagulation in rats. Thromb. Haemostas. 50: 869-872, 1983.   DOI
34 Abei, H. Catalase in vitro. Methods in Enzymol. 105: 93-127, 1984.   DOI
35 Farber, J. L,. GILL, G., Konishi, Y. Prevention of galactosamine- induced liver cell necrosis by uridine. Am. J. Pathol., 72: 53-62, 1973.