Browse > Article

Effect of Sopung-tang on Glutamate-Induced Apoptosis in C6 Glial Cells  

Jeong, Seung-Won (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Choi, Chul-Won (Department of Internal Medicine, School of Oriental Medicine, Wonkwang University)
Kim, Bong-Sang (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Moon, Byung-Soon (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.6, 2008 , pp. 1423-1430 More about this Journal
Abstract
The water extract of Sopung-tang(SPT) has been traditionally used for treatment of psycologic disease and brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of SPT rescues cells from these disease. Therefore, this study was designed to investigate the effect of SPT on the glutamate-induced toxicity of rat C6 glial cells. SPT have protective effects in glutamate-induced toxicity, which was revealed as apoptosis characterized by chromatic condensation and fragmentation and the loss of mitochondrial membrane potential in C6 glial cells. Also, SPT have inhibited the active form of caspase-3 and PARP and significantly protected the apoptotic phenomena by glutamate toxicity in C6 glial cells. However, SPT significantly recovered the depletion of GSH and inhibited the generation of ROS by glutamate in C6 glial cells. In addition, both SPT and antioxidants such as GSH and NAC protected the glutamate-induced cytotoxicity in C6 glial cells, indicating that SPT possibly have antioxidative effect. Specially, SPT were showed transcriptional factor significantly increased the activation of NF-${\kappa}B$ using the analysis of NF-${\kappa}B$ luciferase reporter system in C6 glial cells. These NF-${\kappa}B$ activation protected cells from glutamate-induced toxicity to generate the heme oxygenase-1(HO-1). Taken together, we suggest that SPT have protective effects in glutamate-induced toxicity via a antioxidative mechanism.
Keywords
glutamate; apoptosis; glial cell; Sopung-tang(SPT);
Citations & Related Records
연도 인용수 순위
  • Reference
1 龔廷賢. 萬病回春. 서울, 杏林書院, p 49, 1975.
2 康命吉. 濟衆新編. 서울, 驪江出版社, p 26, 1992.
3 김정제. 診療要鑑. 서울, 東洋醫學硏究院, p 453, 2007.
4 東醫寶鑑國譯硏究會. 東醫寶鑑 許俊著. 서울, 法仁文化社, p 951, 2000.
5 尹用甲. 東醫方劑와 處方解說. 서울, 醫聖堂, p 624, 1998.
6 이민구. 소풍탕이 Glutamate에 의한 C6 glial cell 세포 손상에서 항산화 효소 발현에 미치는 영향. 원광대학교대학원, 2006.
7 추민규. 허혈성 뇌손상 흰쥐에서 인지 및 운동기능회복에 미치는 소풍탕의 효과. 동신대학교대학원, 2008.
8 Peter, L. Ischemic Cell Death in Brain Neurons, Physiol. Review, 70: 1499, 1999.
9 이지현. Protective effects of Daebojungtang on zinc-induced apoptosis in C6 glial cells. 원광대학교 한의학전문대학원, 2000.
10 Baeuerle, P.A., and Henkel, T. Function and activation of NF-$\kappa$B in the immune system, Ann. Rev. Immunol. Today, 12: 141-179, 1994.   DOI   ScienceOn
11 Widmann, C., Gibson, S. Caspase-dependent Cleavage of signaling proteins during apoptosis, J. Biol. Chem. 273(12):7141-7147, 1998.   DOI   ScienceOn
12 金都煥. Nitric Oxide에 의해 유발된 C6 glial 細胞毒性에 대한 四物湯의 防禦效果. 서울, 대한한방내과학회지 21(4): 535-542, 2000.
13 崔錫珍. 疎風湯이 고혈압과 동맥경화에 미치는 영향. 세명대학교대학원, 2004.
14 裵秉哲. 標準臨床 方劑學, 成輔社, p 401, 1995.
15 權寧哲. 疎風湯 및 加味疎風湯이 고지혈증에 미치는 영향. 경희대학교대학원, 1982.
16 金堯漢. 攝生散이 C6 glial 세포의 NO 生成에 미치는 影響. 원광대학교 대학원, 2000.
17 Behl, C., Dabis, J.B., Killer, F.G., Schubert, D. Amyloid beta peptide induces necrosis rather than apoptosis, Brain Res. 645: 253-264, 1994.   DOI   ScienceOn
18 Choi, DW. Maulucci-Gedde, MA., Kriegstein, AR. Glutamate neurotoxicity in cortical cell culture, J. Neurosci. 7: 357-368, 1987.   DOI
19 나영훈. 炙甘草湯이 LPS와 PMA에 의해 損傷된 C6 glial 細胞에 미치는 影響. 원광대학교 대학원, 2000.
20 Kim, Y.M., Talanian, R.V., Billiar, T.R. Nitric oxide inhibits apoptosis by preventing increase in caspase-3 like activity via distinct mechanism, J. Biol. Chem. 272: 311-338, 1997.   DOI   ScienceOn
21 Siebenlist, U., Franzo, G. and Brown, K. Structure, regulation and function of NF-$\kappa$B, Ann Rev. Immunol. 12: 405-455, 1994.
22 Cho, Y., Bannai, S. Uptake of glutamate and cystein in C6 glioma cells and in cultured astrocytes, J. Neurochem, 55: 2091-2097, 1990.   DOI
23 Weiss, J., Hartley, D., Koh, J., Choi, D. AMPA receptor activation potentiates zinc neurotoxicity, Neuron, 10: 43-49, 1993.   DOI   ScienceOn
24 Weiss, J., Hartley, D., Koh, J., Choi, D. AMPA receptor activation potentiates zinc neurotoxicity, Neuron, 10: 43-49, 1993.   DOI   ScienceOn
25 Buttke, T., and Sandstrom, P. Oxidative stress as a mediator of apoptosis, Immunol. Today, 15: 7-10, 1994.   DOI   ScienceOn
26 이영구, 문병순. 右歸飮이 Zinc에 의한 신경교세포의 고사에 미치는 영향. 大韓韓醫學會誌 22(3):63-73, 2001.
27 Cole, S.P. Rapid chemosensitivity testing of human lung tumor cells using the MTT assay. Cancer Chemother Pharmacol. 17: 159-163, 1986.
28 Lemos, F.B., Ijzermans, J.N., Zondervan, P.E., Peeters, A.M., Van Den Engel, S., Mol W.M., Weimar W., Baan C.C. Differential expression of heme oxygenase-1 and vascular endothelial growth factor in cadaveric and living donor kidneys after ischemia-reperfusion. J. Am. Soc. Nephrol. 14(12):3278-3287, 2003.   DOI   ScienceOn
29 鄭章均․安日會. 疎風湯이 腦血栓症 初期 患者에 미치는 영향. 원광한의학, 5(1):181-195, 1995.
30 Klaus, S.O., Davide, F. Apoptosis signal by death receptors, Eur. J. Biochem. 254: 439-459, 1998.   DOI   ScienceOn