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Inhibitory Effect of Chan-Su on the Secretion of PGE2 and NO in LPS-stimulated BV2 Microglial Cells  

Kim, Min-Hee (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Lyu, Ji-Hyo (Clinical Research Center of Oriental Medicine, Dongeui University)
Lyu, Sun-Ae (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Hong, Sang-Hoon (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Kim, Won-Il (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Yoon, Hwa-Jung (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Ko, Woo-Shin (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.5, 2008 , pp. 1315-1321 More about this Journal
Abstract
Chan-Su (Venenum bufonis) has long been for a variety of other purposes including treatment of inflammation in the folk medicine recipe. Since nitric oxide (NO) is one of the major inflammatory parameters, we first studied the effects of Chan-Su on NO production in lipopolysaccharide (LPS)-stimulated BV2 microglial cells, Chan-Su inhibited the secretion of NO in BV2 microglial cells, without affecting cell viability, The protein level of inducible nitric oxide synthase (iNOS) was decreased by Chan-Su, And Chan-Su also inhibited production of prostaglandin E2 (PGE2) and expression of cyclooxygenase (COX)-2. Proinflammatory cytokines, such as tumor necrosis factor $(TNF)-{\alpha}$, interleukin $(IL)-1{\beta}$ and IL-12, were inhibited by Chan-Su in a dose-dependent manner. And Chan-Su inhibited the degradation of ${IkB-\alpha}$, which was considered to be inhibitor of nuclear factor $(NF)-{\kappa}B$, one of a potential transcription factor for the expression of iNOS, COX-2 and proinflammatory cytokines. These results suggest that Chan-Su could exert its anti-inflammatory actions by suppressing the synthesis of NO through inhibition of $I{\kappa}B-{\alpha}$ degradation.
Keywords
Chan-Su(Venenum bufonis); nitric oxide; inducible nitric oxide synthase; cyclooxygenase-2; inhibitor kappa B;
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1 Lee, S.I., Ahn, D.K., Shin, M.K. Clinical application of herb, SeungBo press, Seoul, p 574, 1982
2 Streit, W.J., Mrak, R.E., Griffin, W.S. Microglia and neuroinflammation: a pathological perspective. J Neuroinflammation, 1: 14, 2004   DOI   ScienceOn
3 Vane, J.R., Mitchell, J.A., Appleton, I., Tomlinson, A., Bishop-Bailey, D., Croxtall J., Willoughby, D.A. Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation. Proc Natl Acad Sci. 91(6):2046-2050, 1994   DOI   ScienceOn
4 Murata, T., Ushikubi, F., Matsuoka, T., Hirata, M., Yamasaki, A., Sugimoto, Y., Ichikawa, A., Aze, Y., Tanaka, T., Yoshida, N., Ueno, A., Oh-ishi, S., Narumiya, S. Altered pain perception and inflammatory response in mice lacking prostacyclin receptor. Nature 388: 678-682, 1997   DOI   ScienceOn
5 Shin, M.K., An, D.K., Lee, S.I. Clinical Application of Oriental Medicine, Sungbosa, Seoul, p 71, 1982
6 Moncada, S., Palmer, R.M., Higgs, E.A. Nitric oxide: Physiology, pathophysiology, and pharmacology. Pharmacol Rev. 43: 109-142, 1991
7 Forstermann, U., Closs, E.I., Pollock, J.S., Nakane, M., Schwarz, P., Gath, I., Kleinert, H. Nitric oxide synthase isozymes. Characterization, purification, molecular cloning, and functions. Hypertension 23: 1121-1131, 1994   DOI   ScienceOn
8 Candelario-Jalil, E., Gonzalez-Falcon, A., Garcia-Cabrera, M., Leon, O.S., Fiebich, B.L. Wide therapeutic time window for nimesulide neuroprotection in a model of transient focal cerebral ischemia in the rat. Brain Res. 1007: 98-108, 2004   DOI
9 Munoz-Fernandez, M.A., Fernandez, M.A., Fresno, M. Synergism between tumor necrosis factor-alpha and interferon-gamma on macrophage activation for the killing of intracellular Trypanosoma cruzi through a nitric oxide- dependent mechanism. Eur J Immunol. 22: 301-307, 1992   DOI   ScienceOn
10 Muller, J.M., Loms Ziegler-Heitbrock, H.W., Baeuerle, P.A. Nuclear factor kappa B, a mediator of lipopolysaccharide effects. Immunobiol. 187: 233-256, 1993   DOI
11 Xie, Q.W., Kashiwabara, Y., Nathan, C. Role of transcription factor NF-${\kappa}B$/Rel in induction of nitric oxide synthase. J Biol Chem. 269: 4705-4708, 1994
12 Chang, H.K., Yang, H.Y., Lee, T.H., Shin, M.C., Lee, M.H., Shin, M.S., Kim, C.J., Kim, O.J., Hong, S.P. Cho, S. Armeniacae semen extract suppresses lipopolysaccharide- induced expressions of cyclooxygenase [correction of cycloosygenase]-2 and inducible nitric oxide synthase in mouse BV2 microglial cells. Biol Pharm Bull. pp 449-454, 2005
13 Fiebich, B.L., Mueksch, B., Boehringer, M., Hull, M. Interleukin-1beta induces cyclooxygenase-2 and prostaglandin E(2) synthesis in human neuroblastoma cells: involvement of p38 mitogen-activated protein kinase and nuclear factor-kappa B. J Neurochem. 75: 2020-2028, 2000   DOI   ScienceOn
14 O'Banion, M.K., Miller, J.C., Chang, J.W., Kaplan, M.D., Coleman, P.D. Interleukin-1b induces prostaglandin G/H synthase-2 (cyclooxygenase-2) in primary murine astrocyte cultures. J Neurochem. 66: 2532-2540, 1996   DOI   ScienceOn
15 Trinchieri, G. Proinflammatory and immunoregulatory functions of interleukin-12. Int Rev Immunol. 16: 365-396, 1998   DOI   ScienceOn
16 Karin M., Ben-Neriah Y. Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity. Annual Review of Immunology 18: 621-663, 2000   DOI   ScienceOn
17 Mayer, B., Hemmens, B. Biosynthesis and action of nitric oxide in mammalian cells. Trends Biochem Sci. 22(12):477-481, 1997   DOI
18 Ko, W.S., Park, T.Y., Park, C., Kim, Y.H., Yoon, H.J., Lee, S.Y., Hong, S.H., Choi, B.T., Lee, Y.T., Choi, Y.H. Induction of apoptosis by Chan Su, a traditional Chinese medicine, in human bladder carcinoma T24 cells. Oncol Rep. 14(2):475-480, 2005
19 Kroncke, K.D., Fehsel, K., Kolb-Bachofen, V. Nitric oxide: cytotoxicity versus cytoprotection-how, why, when, and where? Nitric Oxide 1(2):107-120, 1997   DOI   ScienceOn
20 Bauer, M.K., Lieb, K., Schulze-Osthoff, K., Berger, M., Gebicke-Haerter, P.J., Bauer, J., Fiebich, B.L. Expression and regulation of cyclooxygenase-2 in rat microglia. Eur J Biochem. 243: 726-731, 1997   DOI   ScienceOn
21 Minghetti, L., Levi, G. Induction of prostanoid biosynthesis by bacterial lipopolysaccharide and isoproterenol in rat microglial cultures. J Neurochem. 65: 2690-2698, 1995   DOI
22 Schmidt, H.H.H.W., Kelm, M. Determination of nitrite and nitrate by the Griess reaction. In: Methods in Nitric Oxide research, John Wiley, Sons Ltd., pp 491-497, 1996
23 Marletta, M.A., Yoon, P.S., Iyengar, R., Leaf, C.D., Wishnok, J.S. Macrophage oxidation of L-arginine to nitrite and nitrate: Nitrite oxide is an intermediate. Biochem. 27: 8706-8711, 1998
24 Chen, K.K., Kovarikove, A. Pharmacology and toxicology of toad venom. Journal of Pharmaceutical Science 56: 1535-1541, 1967   DOI
25 Yui, Y., Hattori, R., Kosuga, K., Eizawa, H., Hiki, K., Kawai, C. Purification of nitric oxide synthase from rat macrophages. J Biol Chem. 266: 12544-12547, 1991
26 Baldwin, A.S. The NF-$\kappa$B and I-$\kappa$B proteins: new discoveries and insights. Annu Rev Immunol. 14: 649-683, 1996   DOI   ScienceOn
27 Nakajima, K., Kohsaka, S. Functional roles of microglia in the central nervous system. Hum Cell. 11(3):141-155, 1998
28 Skehan, P. Assays of cell growth and cytotoxicity. In: Studzinski, G.P. (Eds.), Cell growth and apoptosis. Oxford University press, New York, p 180, 1998
29 Harbrecht, B.G., Di Silvio, M., Demetris, A.J., Simmons, R.L., Billiar, T.R. Tumor necrosis factor-alpha regulates in vivo nitric oxide synthesis and induces liver injury during endotoxemia. Hepatology 20: 1055-1060, 1994   DOI   ScienceOn
30 Karin M., Delhase M. The IkB kinase (IKK) and NF-kB: key elements of proinflammatory signaling. Seminars in Immunology 12: 85-98, 2000
31 Akiyama, H., Barger, S., Barnum, S., Bradt, B., Bauer, J., Cole, G.M., Cooper, N.R., Eikelenboom, P., Emmerling, M., Fiebich, B.L. Inflammation and Alzheimer's disease. Neurobiol Aging. 21: 383-421, 2000   DOI   ScienceOn
32 Ghosh, S., May M.J., Kopp, E.B. The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immunol. 16: 225-260, 1998   DOI   ScienceOn
33 Benveniste, E.N. Role of macrophages/microglia in multiple sclerosis and experimental allergic encephalomyelitis. J Mol Med. 75(3):165-173, 1997   DOI   ScienceOn
34 Adler, K.B., Fischer, B.M., Wrigh,t D.T., Cohn, L.A., Becker, S. Interactions between respiratory epithelial cells and cytokines: relationships to lung inflammation. Ann N Y Acad Sci. 725: 128-145, 1994   DOI
35 Fiebich, B.L., Biber, K., Lieb, K., van Calker, D., Berger, M., Bauer, J., Gebicke-Haerter, P.J. Cyclooxygenase-2 expression in rat microglia is induced by adenosine A2a-receptors. Glia. 18: 152-160, 1996   DOI   ScienceOn