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Effect of Garlic Extract on the Activation Pattern of MAPK Signaling in the Rat Heart After a Bout Exercise  

Lee, Jun-Hyuk (Chemistry and Biotechnology Examinations Bureau, Korean Intellectual Property Office)
Chung, Kyung-Tae (Department of Biomedical Laboratory Science, College of Natural Science)
Lee, Yang-Tae (Department of Physiology, College of Oriental Medicine, Dong-Eui University)
Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-Eui University)
Choi, Byung-Tae (School of Oriental Medicine, Pusan National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.5, 2008 , pp. 1299-1303 More about this Journal
Abstract
Since exercise training induces mechanical stress to the heart, we examined the activation pattern of mitogen-activated protein kinase(MAPK)s signaling pathway by immunohistochemistry. The immunoreactions of MAPKs signaling with c-fos and Schiff's reaction were increased in the cardiac muscle of exercised rat compared to normal one except immunoreaction for MEK1/2 and ERK1/2 and p38. However, the immunoreaction of phospho-JNK and phospho-p38 with early gene c-fos were arrested markedly in water extract of Alliium sativum (WEAS) treated rat compared to exercised one. Since MAPKs signaling does play a protective role in response to pathological stimulus in the heart, results in the present study suggest that WEAS may act as a alleviating agent for exercise-induced stress to. heart through regulating MAPKs signaling activation.
Keywords
Alliium sativum; cardiac muscle; c-fos; mitogen-activated protein kinase;
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1 Morihara, N., Nishihama, T., Ushijima, M., Ide, N., Takeda, H., Hayama, M. Garlic as an anti-fatigue agent. Mol. Nutr. Food Res. 51: 1329-1334, 2007   DOI   ScienceOn
2 Seger, R., Krebs, E.G. The MAPK signaling cascade. FASEB J. 9: 726-735, 1995   DOI
3 Heineke, J., Molkentin, J.D. Regulation of cardiac hypertrophy by intracellular signalling pathways. Nat. Rev. Mol. Cell Biol. 7: 589-600, 2006   DOI
4 Yeh, Y.Y., Yeh, S.M. Garlic reduces plasma lipids by inhibiting hepatic cholesterol and triacylglycerol synthesis. Lipids 29: 189-193, 1994   DOI   ScienceOn
5 Saxena, K,K., Gupta, B., Kulshrestha, V.K., Srivastava, R.K., Prasad, D.N. Effect of garlic pretreatment on isoprenaline-induced myocardial necrosis in albino rats. Indian J. Physiol. Pharmacol. 24: 233-236, 1980
6 Cornelius, T., Holmer, S.R., Müller, F.U., Riegger, G.A., Schunkert, H. Regulation of the rat atrial natriuretic peptide gene after acute imposition of left ventricular pressure overload. Hypertension 30: 1348-1355, 1997   DOI   ScienceOn
7 Pedraza-Chaverrí, J., Tapia, E., Medina-Campos, O.N. Angeles G.M., Franco, M. Garlic prevents hypertension induced by chronic inhibition of nitric oxide synthesis. Life Sci. 62: 71-77, 1998
8 Maeda, S., Miyauchi, T., Iemitsu, M., Tanabe, T., Yokota, T., Goto, K., Yamaguchi, I., Matsuda, M. Effects of exercise training on expression of endothelin-1 mRNA in the aorta of aged rats. Clin. Sci. 103: 118S-123S, 2002   DOI
9 Pearson, W., Boermans, H.J., Bettger, W.J., McBride, B.W., Lindinger, M.I. Association of maximum voluntary dietary intake of freeze-dried garlic with Heinz body anemia in horses. Am. J. Vet. Res. 66: 457-465, 2005   DOI   ScienceOn
10 Komuro, I., Kudo, S., Yamazaki, T., Zou, Y., Shiojima, I., Yazaki, Y. Mechanical stretch activates the stress-activated protein kinases in cardiac myocytes. FASEB J. 110: 631-636, 1996
11 Nishimoto, S., Nishida, E. MAPK signalling: ERK5 versus ERK1/2. EMBO Rep. 7: 782-786, 2006   DOI   ScienceOn
12 Morihara, N., Ushijima, M., Kashimoto, N., Sumioka, I., Nishihama, T., Hayama, M., Takeda, H. Aged garlic extract ameliorates physical fatigue. Biol. Pharm. Bull. 29: 962-966, 2006   DOI   ScienceOn
13 Iemitsu, M., Maeda, S., Jesmin, S., Otsuki, T., Kasuya, Y., Miyauchi, T. Activation pattern of MAPK signaling in the hearts of trained and untrained rats following a single bout of exercise. J. Appl. Physiol. 101: 151-163, 2006   DOI   ScienceOn
14 Morihara, N., Ide, N., Sumioka, I., Kyo, E. Aged garlic extract inhibits peroxynitrite-induced hemolysis. Redox Rep. 10: 159-165, 2005   DOI   ScienceOn
15 Farooq, A., Zhou, M.M. Structure and regulation of MAPK phosphatases. Cell Signal 16: 769-779, 2004   DOI   ScienceOn
16 Ruwhof, C., Laarse, A. Mechanical stress-induced cardiac hypertrophy: mechanisms and signal transduction pathways. Cardiovasc. Res. 47: 23-37, 2000   DOI   ScienceOn
17 Takeishi, Y., Huang, Q., Abe, J., Che, W., Lee, J.D., Kawakatsu, H., Hoit, B.D., Berk, B,C., Walsh, R.A. Activation of mitogen-activated protein kinases and p90 ribosomal S6 kinase in failing human hearts with dilated cardiomyopathy. Cardiovasc. Res. 53: 131-137, 2002   DOI   ScienceOn
18 Lim, H., Kubota, K., Kobayashi, A., Seki, T., Ariga, T. Inhibitory effect of sulfur-containing compounds in Scorodocarpus borneensis Becc. on the aggregation of rabbit platelets. Biosci. Biotechnol. Biochem. 63: 298-301, 1999   DOI   ScienceOn
19 Fischer, T.H., Brittain, J., Trabalzini, L., Banes, A.J., White, G.C., Smith, C.J., Nichols, T.C. The ras-binding domain of ral GDS-like protein-2 as a ras inhibitor in smooth muscle cells. Biochem. Biophys. Res. Commun. 305: 934-940, 2003   DOI   ScienceOn
20 Nakamura, A., Yoshida, K., Takeda, S., Dohi, N., Ikeda, S. Progression of dystrophic features and activation of mitogen-activated protein kinases and calcineurin by physical exercise, in hearts of mdx mice. FEBS Lett. 520: 18-24, 2002   DOI
21 Bueno, O.F., Molkentin, J.D. Involvement of extracellular signal-regulated kinases 1/2 in cardiac hypertrophy and cell death. Circ. Res. 91: 776-781, 2002   DOI   ScienceOn
22 Sugden, P.H., Clerk, A. Regulation of mitogen-activated protein kinase cascades in the heart. Adv. Enzyme Regul. 38: 87-98, 1998   DOI   ScienceOn