Browse > Article

Effect of Low Intensity Pulsed Ultrasound in Rat Chondrocyte  

Kim, Eun-Jung (Department of Physical Therapy, Dongshin University)
Kim, Gye-Yeop (Department of Physical Therapy, Dongshin University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.5, 2008 , pp. 1262-1269 More about this Journal
Abstract
Low intensity pulsed ultrasound(LIPUS) is known to accelerate bone regeneration, but the precise cellular signaling mechanism is still unclear. The purpose if this study was to determine the effect of LIPUS on the signaling mechanism of rat chondrocyte. In the explant culture condition, there was inhibition effect of 1 $W/cm^2$ intensity LIPUS on chondrocytes proliferation but chondrocytes proliferation was increased at 0.25 $W/cm^2$ intensity. In addition, western blot analysis of MAPKs showed that LIPUS increased ERK1/2 activity from the 10 min treatment of LIPUS. Hydrogen peroxide($H_2O_2$), resulted in a time- and dose-dependent cell proliferation, which was largely attributed to apoptosis. $H_2O_2$ treatment caused marked sustained nucleus condensation in Hoechst stain. LIPUS and $H_2O_2$ activates phosphorylation of p-ERK1/2 and PD 98059($10^{-5}M$) blocked the effect of LIPUS and $H_2O_2$. Moreover, the synergistic phosphorylation of p44/42 MAPK by $H_2O_2$, LIPUS was selectively inhibited by PD 98059, ERK1/2 inhibitor. In order to determine whether the increase in cell proliferation caused by $H_2O_2$ and LIPUS could be explained by changes in the level of the prostaglandin $E_2$. Our study demonstrated that LIPUS stimulate the cell proliferation via activated phosphorylation of ERK1/2 in condrocyte. LIPUS has anabolic effects on rat cartilage in explant cultures, indicating a potential important method for the treatment of osteoarthritic cartilarge.
Keywords
chondrocyte; ERK1/2; Low Intensity Pulsed Ultrasound(LIPUS);
Citations & Related Records
연도 인용수 순위
  • Reference
1 Felson, D.T., Lawrence, R.C., Hochberg, M.C., McAlindon, T., Dieppe, P.A., Minor, M.A., Blair, S.N., Berman B.M., Fries J.F., Weinberger M., Lorig K.R., Jacobs J.J., Goldberg V. Osteoarthritis: new insights. Part 2: treatment approaches. Ann Intern Med. 133: 726-737, 2000   DOI   ScienceOn
2 Lee, S.W., Lee, H.J., Chung, W.T., Choi, S.M., Rhyu, S.H., Kim, D.K., Kim, K.T., Kim ,J.Y., Kim, J.M., Yoo Y.H. TRAIL induces apoptosis of chondrocytes and influences the pathogenesis of experimentally induced rat osteoarthritis. Arthritis Rheum. 50(2):534-542, 2004   DOI   ScienceOn
3 Mow, V.C.,　Wang, C.C. Some bioengineering considerations for tissue engineering of articular cartilage. Clin Orthop Relat Res. 367: 204-223, 1999   DOI
4 Chang, L., Karin, M. Mammalian MAP kinase signalling cascades. Nature 410(6824):37-40, 2001   DOI   ScienceOn
5 Wang, X., Martindale, J.L., Liu, Y., Holbrook, N.J. The cellular response to oxidative stress: influences of mitogen-activated protein kinase signalling pathways on cell survival. Biochem J. 333: 291-300, 1998   DOI
6 Chapman, I.V., MacNally, N.A., Tucker, S. Ultrasound- induced changes in rates of influx and efflux of potassium ions in rat thymocytes in vitro. Ultrasound Med Biol. 6(1):47-58, 1980   DOI   ScienceOn
7 Rubin, C., Bolander, M., Ryaby, J.P., Hadjiargyrou, M. The use of low-intensity ultrasound to accelerate the healing of fractures. J. Bone Joint Surg Am. 83A(9):259-270, 2001
8 Asada, S., Fukuda, K., Nishisaka, F., Matsukawa, M., Hamanisi C. Hydrogen peroxide induces apoptosis of chondrocytes; involvement of calcium ion and extracellular signal-regulated protein kinase. Inflamm Res. 50(1):19-23, 2001   DOI   ScienceOn
9 Takahashi T., Ogawa Y., Kitaoka K., Tani T., Uemura Y., Taguchi H., Kobayashi T., Seguchi H., Yamamoto H., Yoshida S. Selective COX-2 inhibitor regulates the MAP kinase signaling pathway in human osteoarthritic chondrocytes after induction of nitric oxide. Int J. Mol Med. 15(2):213-219, 2005
10 Mitrovic, D., Quintero M., Stankovic, A., Ryckewaert, A. Cell density of adult human femoral condylar articular cartilage. Joints with normal and fibrillated surfaces. Lab Invest 49(3):309-316, 1983   DOI   ScienceOn
11 Que, F.G., Gores, G.J. Cell death by apoptosis: basic concepts and disease relevance for the gastroenterologist. Gastroenterology. 110(4):1238-1243, 1996   DOI   ScienceOn
12 Yang, K.H., Choi, C.H., Cho, J.H. Stimulation of fracture healing by low intensity pulsing ultrasound. The Journal of the Korean Society of Fracture 11(2):247-253, 1998   DOI
13 Hashimoto, S., Setareh, M., Ochs, R.L., Lotz, M. Fas/Fas ligand expression and induction of apoptosis in chondrocytes. Arthritis Rheum. 40(10):1749-1755, 1997   DOI   ScienceOn
14 Schortinghuis, J., Bronckers, A.L., Stegenga, B., Raghoebar, G.M. de Bont, L.G. Ultrasound to stimulate early bone formation in a distraction gap: a double blind randomised clinical pilot trial in the edentulous mandible. Arch Oral Biol. 50(4):411-420, 2005   DOI   ScienceOn
15 Gardner, D.L. Problems and paradigms in joint pathology. J Anat. 184: 1749-1755, 1994
16 Pelletier, J.P., Jovanovic, D.V., Lascau-Coman, V, Fernandes, J.C., Manning, P.T., Connor, J.R., Currie, M.G., Martel- Pelletier, J. Selective inhibition of inducible nitric oxide synthesis reduces progression of experimental osteoarthritis in vivo. Arthritis Rheum. 43(6):1290-1299, 2000   DOI   ScienceOn
17 Zhou, S., Schmelz, A., Seufferlein, T., Li, Y., Zhao, J., Bachem M.G. Molecular mechanisms of low intensity pulsed ultrasound in human skin fibroblasts. J. Biol Chem. 279(52):54463-54469, 2004   DOI   ScienceOn
18 Xia, Z., Dickens, M., Raingeaud, J., Davis, R.J., Greenberg, M.E. Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science 270(5240):1326-1331, 1995   DOI
19 Cook, S.D., Ryaby, J.P., McCabe, J., Frey, J.J., Heckman, J.D., Kristiansen T.K. Acceleration of tibia and distal radious fracture healing in patients who smoke. Clin Orthop. 337: 198-207, 1997   DOI
20 Choi, B.H., Choi, M.H., Kwak, M.G., Min, B.H., Woo, Z.H., Park S.R. Mechanotransduction pathways of low-intensity ultrasound in C-28/I2 human chondrocyte cell line. Proc Inst Mech Eng. 221(5):527-535, 2007
21 Chang, W., Tu, C., Chen, T.H, Komuves, L., Oda, Y., Pratt, S.A., Miller S., Shoback D. Expression and signal transduction of calcium-sensing receptors in cartilage and bone. Endocrinology 140(12):5883-5893, 1999   DOI   ScienceOn
22 Parvizi, J., Wu, C.C., Lewallen, D.G., Greenleaf, J.F., Bolander, M.E. Low-intensity ultrasound stimulates proteoglycan synthesis in rat chondrocytes by increasing aggrcan gene expression. J. Orthop Res. 17(4):488-494, 1997   DOI
23 Stadheim, T.A., Kucera, G.L. Extracellular signal-regulated kinase (ERK) activity is required for TPA-mediated inhibition of drug-induced apoptosis. Biochem Biophys Res Commun 245(1):266-271, 1998   DOI   ScienceOn
24 Kumar, A., Chaudhry, I., Reid, M.B., Boriek, A.M. Distinct signaling pathways are activated in response to mechanical stress applied axially and transversely to skeletal muscle fibers. J. Biol Chem. 277(48):46493-46503, 2002   DOI   ScienceOn
25 Doan, N., Reher, P., Meghji, S., Harris, M. In vitro effects of therapeutic ultrasound on cell proliferation, protein synthesis, and cytokine production by human fibroblasts, osteoblasts, and monocytes. J. Oral Maxillofac Surg. 57(4):409-419, 1999   DOI   ScienceOn
26 Chen, Y.J., Wang, C.J., Yang, K.D., Chang, P.R., Huang, H.C., Huang, Y.T., Sun Y.C., Wang F.S. Pertussis toxin-sensitive Galphai protein and ERK-dependent pathways mediate ultrasound promotion of osteogenic transcription in human osteoblasts. FEBS Lett. 554(1-2):154-158, 2003   DOI
27 DiPietrantonio, A.M., Hsieh, T., Wu, J.M. Activation of caspase 3 in HL-60 cells exposed to hydrogen peroxide. Biochem Biophys Res Commun. 255(2):477-482, 1999   DOI   ScienceOn
28 Huang, M.H., Ding, H.J., Chai, C.Y., Huang, Y.F., Yang R.C. Effects of sonication on articular cartilage in experimental osteoarthritis. J. Rheumatol. 24(10):1978- 1984, 1997
29 Naruse, K., Miyauchi, A., Itoman, M., Mikuni-Takagaki, Y. Distinct anabolic response of osteoblast to low-intensity pulsed ultrasound. Bone Miner Res. 18(2):360-369, 2003   DOI   ScienceOn
30 Pouysségur, J., Volmat, V., Lenormand, P. Fidelity and spatio-temporal control in MAP kinase (ERKs) signalling. Biochem Pharmacol. 64(5-6):755-763, 2002   DOI
31 Cano, E., Mahadevan, L.C. Parallel signal processing among mammalian MAPKs. Trends Biochem Sci. 20(3):117-122, 1995   DOI
32 Setton, L.A., Mow, V.C., Muller, F.J., Pita, J.C., Howell, D.S. Mechanical behavior and biochemical composition of canine knee cartilage following periods of joint disuse and disuse with remobilization. Osteoarthritis Cartilage 5(1):1-16, 1997   DOI   ScienceOn
33 Raucci, A., Laplantine, E., Mansukhani, A., Basilico, C. Activation of the ERK1/2 and p38 mitogen-activated protein kinase pathways mediates fibroblast growth factor-induced growth arrest of chondrocytes. J. Biol Chem. 279(3):1747-1756, 2004   DOI   ScienceOn
34 Reyes-Martin, P., Alique, M., Parra, T., Hornedo, J.P., Lucio-Cazana J. Cyclooxygenase-independent inhibition of $H_2O_2$-induced cell death by S-ketoprofen in renal cells. Pharmacol Res. 55(4):295-302, 2007   DOI   ScienceOn
35 Hannan, M.T., Anderson, J.J., Zhang, Y., Levy, D., Felson, D.T. Bone mineral density and knee osteoarthritis in elderly men and women. The Framingham Study. Arthritis Rheum 36(12):1671-1680, 1993   DOI   ScienceOn