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Effect of Samjahwadam-jeon on CD4, CD8 Cells in OVA-induced Asthmatic Mice  

Nam, Tae-Heung (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Park, Yang-Chun (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.5, 2008 , pp. 1256-1261 More about this Journal
Abstract
This study aimed to examine the effects of Samjahwadam-jeon (SJHDJ) on $CD4^+$ T cells and $CD8^+$ T cells in ovalbumin (OVA)-induced asthmatic mice. C57BL/6 mice were injected, inhaled and sprayed with OVA for 12 weeks (four a week) for asthma induction. Two experimental groups were treated with different concentrations of SJHDJ (400 mg/kg and 200 mg/kg) extract and cyclosporin A (10 mg/kg) for the later 8 weeks. At the end of the experiment, the mice lung, peripheral lymph node (PLN), and spleen were removed and $CD4^+$ T cells and $CD8^+$ T cells for analyzed by flow cytometer. Number of $CD4^+$ T cells in lung, PLN and spleen of the SJHDJ group (400 mg/kg and 200 mg/kg) were significantly decreased compared with that of control group. Number of $CD8^+$ T cells in PLN and spleen of the SJHDJ group (400 mg/kg, 200 mg/kg) were significantly decreased compared with that of control group. Conclusion: These results suggest that SJHDJ alleviated asthmatic hyperreactivity through $CD4^+$ and $CD8^+$ T cells. Further study of relative cytokines is expected.
Keywords
Samjahwadam-jeon (Sanzihuatan-jian); asthma$CD4^+$ T cells$CD8^+$ T cells;
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