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Inhibitory Effect of Sorbus cortex Extract on (1,3)-$\beta$-Glucan Synthase  

You, Myung-Ja (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University)
Kim, Bo-Mi (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University)
Lee, Jeong-Ho (Department of Natural Health Management, Songho College)
Lee, Young-Hang (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University)
Chai, Kyu-Yun (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University)
Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.5, 2008 , pp. 1196-1201 More about this Journal
Abstract
A examination of the kinetic properties of UDP-glucose : (1,3)-$\beta$-glucan (callose) synthase from mung bean seedings (Sorbus cortex) shows that these enzymes have a complex interaction with UDP-glucose and various effectors. Deoxynojirimycin increased the inhibitory effect of (1,3)-$\beta$-glucan synthase at the concentration-dependent manner by fluorescence assay. The inhibitory effect of Fr. 2-16 (97.15%) showed higher than that of deoxynojirimycin (80.63%). Fr. 2-3 inhibited the growth of the Candida albicans at 1 mm inhibition zone by disk diffusion method. These results suggest that Sorbus cortex extract can be used as a stable antifungal material.
Keywords
Kinetic properties; (1,3)-$\beta$-glucan (callose) synthase; Sorbus cortex; fluorescence assay; inhibitory effect; Candida albicans;
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