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Radical Scavenging Activity of Gallic Acid from Woodfordia Fruticosa Flowers  

Bhatt, Lok Ranjan (Department of Bionanochemistry, Wonkwang University)
Yook, Chan-Nam (Department of Food Nutrition, Wonkwang Health Sciences)
Choi, Hwa-Jung (Natural Medicine Research Centre, Korea Research Institute of Bioscience & Biotechnology)
Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.4, 2008 , pp. 903-906 More about this Journal
Abstract
Woodfordia fruticosa Kurz (Lythraceae) is used in the treatment of various ailments in traditional medicines. DPPH activity guided fractionation and purification process was used to identify the free radical-scavenging components from the flowers of this plant. The methanolic extract of the plant was first fractionated into four extracts; namely, n-hexane, chloroform, ethyl acetate and water fractions. Among them, the ethyl acetate fraction was found to be the most effective and was further subjected to activity guided-fractionation and isolation procedures. After successive column chromatography on silica gel and Sephadex LH-20, gallic acid, which is responsible for the radical scavenging activity, was isolated and its structure was elucidated by spectral methods ($^1H$ NMR, $^{13}C$ NMR) and by comparison with literature.
Keywords
Woodfordia fruticosa; antioxidants; gallic acid; DPPH; HPLC;
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