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Effect of Water Extract of Deer Antler in Osteoclast Differentiation  

Kwak, Han-Bok (Department of Anatomy, School of Medicine, and Wonkwang Medical Science Institute)
Kim, Ju-Ho (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute)
Kim, Dong-Joo (Department of Anatomy, School of Medicine, and Wonkwang Medical Science Institute)
Kwon, Young-Mi (Department of Diagnostic Radiology, Oriental Medicine, Wonkwang University)
Oh, Jae-Min (Department of Anatomy, School of Medicine, and Wonkwang Medical Science Institute)
Kim, Yun-Kyung (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.4, 2008 , pp. 891-895 More about this Journal
Abstract
Natural substances have recently received much attention as therapeutic drugs to prevent many diseases in humans because they avoid the many side effects of treatment with chemical compounds. We examined the effect of water extract of deer antler in RANKL-induced osteoclast differentiation. The effects of water extract of deer antler in osteoclast differentiation were determined by culture of bone marrow macrophages (BMMs). The mRNA expression levels of c-Fos, NFATc1, TRAP, and GAPDH in BMMs were analyzed by RT-PCR. Cell lysates were obtained from the treated cells, the expression levels of c-Fos and NFATc1 were determined by western blotting with antibodies for c-Fos and NFATc1. Water extract of deer antler greatly inhibited RANKL-mediated osteoclast differentiation in osteoclast precursors without cytotoxicity. Water extract of deer antler inhibited the expression of c-Fos and NFATc1 in BMMs treated with RANKL. Our findings suggest that water extract of deer antler inhibited osteoclast differentiation by suppressing c-Fos and NFATc1 expression in response to RANKL. These results demonstrate that water extract of deer antler may be a useful the treatment of bone-related disease such as osteoporosis.
Keywords
osteoclasts; water extract of deer antler; RANKL; NFATc1;
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