Browse > Article

Inhibitory Effect on Melanogenesis of Ponciri Fructus Ethanol Extract  

Ko, Joon-Suk (Department of Herbology, College of Oriental Medicine, Sangji University)
Lee, Jang-Cheon (Department of Herbology, College of Oriental Medicine, Sangji University)
Jang, Yeong-Mi (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Mun, Yeun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Lim, Kyu-Sang (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Lee, Young-Cheol (Department of Herbology, College of Oriental Medicine, Sangji University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.4, 2008 , pp. 829-834 More about this Journal
Abstract
This study was to investigate the effect of Ponciri Fructus ethanol extract on the melanogenesis. The Ponciri Fructus markedly decreased melanin contents of B16F10 cells at a dose of $80{\mu}g/m{\ell}$. It was observed that the color of cell pellets was totally whitened compared with the control. The treatment of Ponciri Fructus inhibited tyrosinase activity, regulate melanin biosynthesis as the key enzyme in melanogenesis. Regarding protein levels of the melanogenic enzymes, the Ponciri Fructus dose-dependently decreased tyrosinase and TRP-1 protein levels, and tyrosinase and TRP-1 were detected in similar manner. These results suggest that the Ponciri Fructus ethanol extract exerts its depigmenting effects through the suppression of tyrosinase and TRP-1.
Keywords
Ponciri Fructus; melanin; tyrosinase; depigmentation; TRP-1;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 NO, J.K., Soung, D.Y., Kim, Y.J., Shim, K.H., Jun, Y.S., Rhee, S.H., Yokizawa, T., Chung, H.Y. Inhibition of tyrosinase by green tea components. Life Science. 65(21):241-246, 1999
2 Prota, G. Recent advances in the chemistry of melanogenesis in mammals. J. Invest. Dermatol. 75(122):31-36, 1990
3 조미경, 안병상, 문연자, 우원홍. 연교메탄올추출물의 멜라닌생성 억제효과. 대한한방피부미용학회지 1(1):41-52, 2005
4 田溶敏 編. 再編整理 黃帝內經 靈樞, 서울, 圖書出版 東源文化社, p 264, 2002
5 국가중의약관리국 중화본초 편찬위원회. 중화본초. 상해, 상해과학기술출판사, p 874, 875, 876, 877, 878, 879, 880, 1999
6 윤황금 외. 지실의 생리활성성분. 약학회지 36: 548-555, 1992
7 허 준. 대역동의보감. 법인문화사, 서울, p 1978, 1999
8 Kametama, K.T., Takemura, Y., Hamada, C., Sakai, S., Kondoh, S., Nishiyama, K., Urabe and J. Hearing. Pigment production in murine melanoma cell is regulated by tyrosinase, tyrosinase-related protein 1(TRP-1), DOPAchrome tautomerase (TRP-2), and a melanogenic inhibitor. J. Invest. Dermatol. 2: 126-131, 1993
9 Wu, X., Bowers, B., Rao, K., Wei, Q., Hammer, J.A. Visualization of melanosome dynamics within wild-type and dilute melanocytes suggests a paradigm for myosin V function In vivo. J Cell Biol. 143: 1899-1918, 1998   DOI   ScienceOn
10 Lee, S.H., Park, J.S., kim, S.Y., kim, J.J., Chung, S.R. Isolation of inhibitory components oa tyrosinase activity from the bark of Paeonia moutan. Yakhak Hoeji. 42(4):353-358, 1998
11 Imokawa, G., Mishima, Y. Loss of melanogenic properties in tyrosinase induced by glucosylation inhibitors within malignant melanoma cells. Cancer Res. 42: 1994-2002, 1982
12 Hosoi, J.E., Suda, T., Kuroki, T. Regulation of melanin synthesis of B16 mouse melanoma cells by 1$\alpha$,25­dehydroxyvitamin D3 and retinoic acid. Cancer Res. 45: 1474-­1478, 1985
13 임난영, 권강주, 김윤석, 백순기, 임주락, 문연자, 우원홍. 사삼메탄올 추출물의 멜라닌 생성 억제 효과. 동의생리병리학회지 18(3):747-753, 2004
14 신길구. 신씨본초. 수문사, 서울, p 724, 725, 1988
15 Sang Yoon Choi, Nan Ju Kang, Ho Cheol Kim. Inhibitory Effects of Root Extracts on Melanin Biosynthesis in Rodgersia podophylla A. Gray 44: 455-462, 2002
16 Curto, E.V., Kwong, C., Hermersdorfer, H., Glatt, H., Santis, C., Virador, V., Hearing, V.J. and Dooley, P. Inhibitors of mamalian melanocytes tyrosinase : In vitro comparisons of alkyl esters of gentisic acid with other putative ingibitors. Biochemical Pharmacology 57: 663-672, 1999   DOI   ScienceOn
17 Maeda, K., Fukuda, M. In vivo effectiveness of several whitening cosmetic components in human melanocytes. J. Soc. cosmet. Chem. 42: 361-368, 1991
18 강호석 외. 조직학 제2판. 서울, 고문사, p 319, 327, 1994
19 Mosmann, T. Rapid colorlmetric assay for the cellular growth and survival : application to proliferation and cytotoxic assay. J. Immun. Methods 65: 55-63, 1983   DOI
20 Kim, C.W. Experimental study on the hyperpigmentation of skin graft. Plast Reconstr. Surg. 85: 162, 1990
21 Ae Ryang Son, Ji Young Choi, Jeong Ah Kim, Se Hoon Cho, Guang Hua Xu, Sung Hee Park, See Ryun Chung, Tae Chun Chung, Yurng Dong Jahng, Seung Ho Jong. Isolation of Melanogenesis Inhibitors from Ponciri Fructus. Keun Son. 36: 1-8, 2005
22 田溶敏 編. 再編整理 黃帝內經 素問. 서울, 圖書出版 東源文化社, p 101, 619, 2002
23 Kobayashi, T., Imokawa, G., Bennett, D.C., Hearing, V.J. Tyrosinase stabilization by Tyrp1(the brown locus protein). J Biol. Chem. 273: 31805, 1998
24 Provance, D.W., Jr, Wei, M., Ipe, V., Mercer, J.A. Cultured melanocytes from dilute mutant mice exhibit dendritic morphology and altered melanosome distribution. Proc Natl Acad Sci USA 93: 14554-14558, 1996
25 문연자, 김진, 임난영, 이승연, 곽섭, 황충연, 우원홍. 감초 물추출물의 멜라닌 형성 억제효과. 동의생리병리학회지 16(6):1230-1235, 2002
26 이승연, 김진만, 오현철, 임숙정, 황충연, 문연자, 우원홍. 더덕추출물이 멜라닌생성에 미치는 영향. 대한한의학 방제학회지 10(2):199-211, 2002
27 노성택, 김대성, 이장천, 임규상, 신미란, 우원홍, 문연자. 교맥 에탄올 추출물의 멜라닌생성 억제효과. 대한전통의학지 15(1):70-76, 2006
28 이주상, 김정아, 조세훈, 손애량, 장태수, 소명숙, 정시련, 이승호. 감초의 tyrosinase 활성 억제 성분. Kor. J. Pharmacogn. 34(1):33-39, 2003
29 전국한의과대학 본초학교수. 본초학. 영림사, 서울, p 395, 396, 2004
30 문연자, 이성원, 임숙정, 송채석, 이관순, 임규상, 우원홍. 천화분 수추출물이 B16 흑색종 세포의 멜라닌 생성 억제 효과. 한국전통의학지 10(2):149-159, 2000
31 Hara, M., Yaar, M., Byers, H.R., Goukassian, D., Fine, R.E., Gonsalves, J., Gilchrest, B.A. Kinesin participates in melanosomal movement along melanocyte dendrites. J Invest Dermatol. 114: 438-443, 2000   DOI   ScienceOn
32 Nam Chul Cho, Yeon Hee Yoon, Hye Jin Lee, Hyun Jung Shon, Yang Kyung Kim, Keun Hee Choi, Myung Suk Ra, Young Kwon Jo, Hwang Hee, Blaise Lee, Jong Eon Chin. Effect or Onion (Allium cepa. L.) Extract on Tyrosinase Gene Expression. 20(3-57):10-16(7), 2005
33 Mishima, Y., Imokawa, G. Selective aberration and pigment loss in melanosomes of maligment melanoma cells in vitro by glycosylation inhibitors: Premelanosomes as glycoprotein. J. Invest. Dermatol. 81: 106-114, 1983   DOI   ScienceOn
34 조기여, 정연, 조혜명, 채순님, 류현혜, 송애량, 장미혜. 피부과학. 서울, 성화, p 90, 91, 2001
35 천현자, 최은영, 윤성찬, 남항우, 백승화, 우원홍. 백출의 에탄올 추출물에 의한 melanin 생성 억제 효과. 약학회지 45(3):269-275, 2001
36 Martinez­Esparza, M., Jimenez-Cervantes, C., Solano, F., Lozano, J.A., Garcia­Borron, J.C. Mechanism of melanogenesis inhibition by tumor necrosis factor­alpha in B16/F10 mouse melanoma cells, Eur. J. Biochem. 255: 139-146, 1998   DOI   ScienceOn