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Protective Effect of Cordyceps sinensis Extract on Cytokine-induced Cytotoxicity of Pancreatic ${\beta}-cells$  

Song, Je-Ho (Division of Beauty Design & Institute for Better Living, Wonkwang University)
Park, Byung-Hyun (Department of Biochemistry, Medical School and Institute for Medical Sciences, Chonbuk National University)
Ryu, Do-Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kwon, Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.4, 2008 , pp. 791-795 More about this Journal
Abstract
In the present study, Cordyceps sinensis extract (CSE) was evaluated to determine if it could protect pancreatic ${\beta}$ cells against cytokine-induced cytotoxicity of RINm5F cells. Treatment of cells with cytokines resulted in a decrease of viability, which was caused by increase of nitric oxide (NO) production. CSE protected cytokine-mediated viability reduction in a concentration-dependent manner. Incubation with CSE also induced a significant suppression of cytokine-induced inducible nitric oxide synthase (iNOS) protein and NO production. The molecular mechanism by which CSE inhibited iNOS protein expression appeared to involve the inhibition of $NF-{\kappa}B$ activation. The cytokine-stimulated RIN cells showed increases in $NF-{\kappa}B$ binding activity compared to unstimulated cells. However, pretreatment with CSE inhibited cytokines-induced $NF-{\kappa}B$ activation in RINm5F cells.
Keywords
cordyceps sinensis; diabetes; cytokine; nitric oxide;
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Times Cited By KSCI : 2  (Citation Analysis)
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