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Inhibition of Lipopolysaccharide-Induced Expression of Inducible Nitric Oxide and Cyclooxygenase-2 by Aquaous of Aconitum pseudo-laeve var. erectum in RAW 264.7 Macrophages  

Han, Myung-Soo (Department of Oriental Neuropsychiatry, College of Oriental Medicine, Semyung University)
Lee, Jae-Hyok (Department of Oriental Neuropsychiatry, College of Oriental Medicine, Semyung University)
Kim, Ee-Hwa (Department of Oriental Neuropsychiatry, College of Oriental Medicine, Semyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.3, 2008 , pp. 678-683 More about this Journal
Abstract
Aconitum pseudo-laeve var. erectum has traditionally been used for the treatment of water retention in the body. Administration of the aqueous extract of Aconitum pseudo-laeve var. erectum has the efficiency of anti-inflammatory activity and modulates the intestinal immune system. However, the mechanism of anti-inflammatory action of Aconitum pseudo-laeve var. erectum has not been clarified yet. In the present study, the effect of Aconitum pseudo-laeve var. erectum against LPS-stimulated expressions of COX-2 and iNOS in cells of the murine RAW 264.7 macrophages was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription- polymerase chain reaction (RT-PCR), PGE2 immunoassay, and NO detection. The results of the present study indicate that Aconitum pseudo-laeve var. erectum is a potent inhibitor of the LPS-induced NO and $PGE_{2}$ production by blocking iNOS and $NF{\kappa}B$ activation in RAW 264.7 macrophages. These findings suggest that Aconitum pseudo-laeve var. erectum is a potential therapeutic for the treatment of inflammatory syndrome.
Keywords
Aconitum pseudo-laeve var. erectum; Anti-inflammation; iNOS; COX-2$NF{\gamma}B$;
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