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Effect of Aqueous Extract from Asiasari Radix on ${\alpha}$-melanocyte Stimulating Hormone Induced Melanogenesis in B16F10 Melanoma Cells  

Lee, Jun-Hyuk (Chemistry and Biotechnology Examinations Bureau, Korean Intellectual Property Office)
Shin, Dong-Yeok (Departments of Biochemistry, College of Oriental Medicine, Dong-Eui University)
Choi, Yung-Hyun (Departments of Biochemistry, College of Oriental Medicine, Dong-Eui University)
Chung, Kyung-Tae (Departments of Biomedical Laboratory Science, College of Natural Science, Dong-Eui University)
Kang, Byoung-Won (Departments of Chemistry, College of Natural Science, Dong-Eui University)
Jeong, Seong-Yun (Korea BIO-IT Foundry Center)
Choi, Byung-Tae (School of Oriental Medicine, Pusan National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.3, 2008 , pp. 649-653 More about this Journal
Abstract
The aqueous extract from Asiasari radix (AEAR) was used to investigate the effect of ${\alpha}$-melanocyte stimulating hormone induced melanogenesis in B16F10 mouse melnoma cells. The treatment with AEAR at the 1.0 and 2.0 mg/ml level significantly inhibited the biosynthesis of melanin without changes of cell growth and morphology compared with untreated control. The AEAR-treated cells at the 2.0 mg/ml level were more efficient than commercial arbutin at 0.1 mg/ml. The tyrosinase activity also significantly decreased in AEAR-treated cells at the 1.0 and 2.0 mg/ml level. The Western analyses confirmed the slightly decreased expression of tyrosinase by AEAR treatment. These results indicate that AEAR may contribute to the inhibition of melanin biosynthesis through regulating tyrosinase activity and expression and serve as a new candidate in the design of new skin-whitening or therapeutic agents.
Keywords
Asiasari radix; melanogenesis; tyrosinase; tyrosinase-related protein;
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