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Anti-allergic Inflammatory Effects of Hwangyounhaedog-tang on PMA Plus A23187-stimulated RBL-2H3 Cells  

Baek, Sang-In (Department of Oriental Medicine, Graduate School Dong-Eui University)
Bae, Jong-Hoon (Department of Oriental Medicine, Graduate School Dong-Eui University)
Kim, Jin-Young (Department of Oriental Medicine, Graduate School Dong-Eui University)
Sim, Sung-Heum (Department of Oriental Medicine, Graduate School Dong-Eui University)
Kang, Kyung-Hwa (Department of Oriental Medicine, Graduate School Dong-Eui University)
Lee, Seung-Yeon (Department of Oriental Medicine, Graduate School Dong-Eui University)
Kam, Cheol-Woo (Department of Oriental Medicine, Graduate School Dong-Eui University)
Park, Dong-Il (Department of Oriental Medicine, Graduate School Dong-Eui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.3, 2008 , pp. 600-606 More about this Journal
Abstract
The purpose of this study was to investigate Anti-allergic Inflammatory Effects of Hwangyounhaedog-tang on PMA plus A23187-stimulated RBL-2H3 cells. We examined MTT assay, ${\beta}$-hexosaminidase activity, IL-4 and TNF-${\alpha}$ from RBL-2H3 after pre-treatment with HHT. In addition, we investigated expression of COX-1 and COX-2 mRNA using RT-PCR study. The results are as follows: All the concentrations of HHT from 1 to 4 mg/ml didn't have an effect on cell viability. In RBL-2H3 cells, ${\beta}$-hexosaminidase release, IL-4 and TNF-${\alpha}$ were significantly reduced by treated all concentrations of HHT. COX-2 on mRNA expression was reduced at treatment 2mg of HHT prior to stimulating by A23187. These results indicate that HHT has inhibition effects on allergy in this study.
Keywords
Hwangyounhaedog-tang; Anti-allergic Inflammatory Effects;
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1 東醫寶鑑. 여강출판사. p 3, 2420, 1994
2 Halfon, N., Newacheck, P.W. Trends in the hospitalization for acute childhood asthma, 1970-1984. m J Public Health 76(11):1308-1311, 1986
3 Smith, J.M., Harding, L.K., Cumming, G. The changing prevalence of asthma in school children. Clin Allergy 1(1):57-61, 1971   DOI
4 Moon, P.D., Na, J.J., Jeong, H.J., Hong, S.H., Kim, S.J., Chae, H.J., Kim, H.R., Choi, J.O., Lee, S.H., Shin, J.Y., Kim, H.M. Inhibitory effect of Gamibojungikaitang extract on mast cell-mediated allergic reaction in murine Model. J Pharm Pharm Sci 8(1):94-101, 2005
5 강경진, 전병득, 채옥희, 이무삼. 상백피가 흰쥐 복강 비만세포의 히스타민 유리와 Calcium uptake에 미치는 영향. 대한면역학회지 15: 91-99, 1993
6 은희철 외. 피부면역학. 서울대학교출판부, pp 1-2, 1999
7 Schwartz, L.B., Austen, K.F., Wasserman, S.I. Immunologic release of beta-hexosaminidase and beta-glucuronidase from purified rat serosal mast cells. J Immunol 123: 1445-1450, 1979
8 Jirapongsananuruk, O., Leung, D.Y. Clinical applications of ytokines: new directions in the therapy of atopic diseases. Ann Allergy Asthma Immunol. 79(1):5-20, 1997   DOI   ScienceOn
9 동의학사전. 까치, p 211, 404, 411, 416, 516, 804, 1143, 1215, 1990
10 方藥合編. 南山堂, p 132, 2000
11 Mitchell, E.A. Increasing prevalence of asthma in children. N Z Med J. 96(734):463-464, 1983
12 Simmons, D.L., Botting, R.M., Hla, T. Cyclooxygenase isozymes: the biology of prostaglandin synthesis and inhibition. Pharmacol Rev. 56(3):387-437, 2004   DOI   ScienceOn
13 채옥희, 이종인, 이무삼. 상백피의 colchicine 유도 비만세포 활성화 억제효과. 대한해부학회지 32: 735-747, 1999
14 金永錫. 血栓症 및 高點度血症에 對한 當歸飮과 黃連解毒湯의 實驗的 比較 硏究. 경희의학, 7(3), 1991
15 Ennis, M., Pearce, F.L., Weston, P.M. Some studies on the release of histamine from mast cells stimulated with polylysine. Br. J. Pharmacol 70: 329-334, 1980   DOI   ScienceOn
16 Dastych, J., Walczak-Drzewiecka, A., Wyczolkowska, J., Metcalfe, D.D. Murine mast cells exposed to mercuric chloride release granule-associated N-acetyl-beta-d- hexosaminidase and secrete IL-4 and TNF-alpha. J Allergy Clin Immunol 103: 1108-1114, 1999   DOI   ScienceOn
17 김유영, 조상헌, 김우경, 박재경, 김윤근, 송숙희, 지영구, 하미나, 안윤옥, 이상일, 장석일, 민경업. 설문지와 메타콜린 기관지유발시험을 이용하여 조사한 한국의 소아 천식 유병률. 대한알레르기학회지 16(2):175-184, 1996
18 이중달. 그림으로 설명한 병리학. 서울, 고려의학, p 29, 1991
19 Lane, S.J., Lee, T.H. Mast cell effector mechanisms. J Allergy Clin Immunol. 98(5 Pt 2):S67-71, 1996   DOI
20 김동찬, 노승현, 이상인, 이영종, 주영승. 방제학. 서울, 영림사, pp 111-113, 263-264, 1990
21 김형민. 면역과 알레르기. 신일상사, p 136, 1998
22 Ehrlich, P. Beitrage zur kenntnis der Anilinfarbunger und ihrer Verwendung in drmikroskopischen Technik. Arch Mikrosk Anast 13: 263-277, 1877   DOI
23 대한 천식 및 알레르기학회. 천식과 알레르기 질환. 서울, 군자출판사, pp 66, 431-433, 2002
24 공민정 외. 황련해독탕과 및 가미방의 인지기능회복에 대한 연구. 대한본초학회지 19(4), 2004
25 정해영. 생명과학.분자의학을 위한 사이토카인 분자생물학. 월드사이언스, pp 117-124, 2002