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Protective Effect of Radix Clematidis Extract on Streptozotocin-induced Diabetes  

Ham, Kyung-Wan (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kim, Eun-Kyung (Department of Biochemistry, Medical School and Institute for Medical Sciences, Chonbuk National University)
Song, Mi-Young (Department of Biochemistry, Medical School and Institute for Medical Sciences, Chonbuk National University)
Kwon, Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Song, Je-Ho (Division of Beauty Design & Institute for Better Living, Wonkwang University)
Seo, Eun-A (Department of Food and Nutrition, School of Human Envioronmental Science, Wonkwang University)
Ryu, Do-Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.3, 2008 , pp. 580-584 More about this Journal
Abstract
In the present study, Radix clematidis extract (RCE) was evaluated to determine if it could protect pancreatic ${\beta}$ cells against multiple low dose streptozotocin (MLDS)-induced diabetes. Injection of mice with MLDS resulted in hyperglycemia and hypoinsulinemia, which was confirmed by immunohistochemical staining. However, the induction of diabetes by MLDS was completely prevented when mice were pre-administrated with RCE. Generation of oxidative stress is implicated in MLDS, a ${\beta}$ cell specific toxin-induced islet cell death. In this context, to elucidate the mechanisms of protective effects in RCE pre-administrated diabetic mice, we investigated the expression of heme oxygenase-1 (HO-1), which is one of the anti-oxidant enzymes. MLDS-induced HO-1 expressions were significantly reduced in MLDS-treated mice. However, the decrease of HO-1 by MLDS were protected by pretreatment of RCE. The molecular mechanism by which RCE inhibits diabetic conditions by MLDS appears to involve inhibition of HO-1 expression. Taken together, these results reveal the possible therapeutic value of RCE for the prevention of type 1 diabetes progression.
Keywords
Radix clematidis; diabetes; multiple low-dose streptozotocin; heme oxygenase-1;
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