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Effect of Achyrantis Radixs and Ultrasound in Osteoarthritis Rats Articular Cartilage  

Kim, Eun-Jung (Department of Physical Therapy Dongshin University)
Chung, Hun-Woo (Department of Oriental Medicine of Dongshin University)
Kim, Gye-Yeop (Department of Physical Therapy Dongshin University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.2, 2008 , pp. 390-396 More about this Journal
Abstract
Osteoarthritis(OA) diseases are characterized by joint pain, tenderness, limitation of movement, crepitus, occasional effusion, and variable degrees of inflammation without systemic effects. We investigated the effects of Achyrantis radixs cream treatment and low intensity ultrasound in monosodium iodoacetate(MIA) induced experimental osteoarthritis rat. Sprague-Dawley 40 rats of 7-8 weeks, weight $250\;{\pm}\;50$ g were divided into four groups including the control group and ostoarthritis group(30 rats). Histopathological examination, Mankin's score, and immunohistochemical were performed. Histological findings in control group that are similar to those observed in human osteoarthritis, such as disorganization of chondrocytes, erosion and fibrillation of cartilage surface, and subchondral bone exposure. Safranin O-fast green staining revealed that marked diffuse reduction of proteoglycans and chondrocyte treated with MIA. The Mankin's score were closely correlated to the grade of histological findings. The level of Bax and caspase-3 expression decreased experimental groups. This study shows that a Acyranthes Radix cream treatment and low intensity ultrasound exerts a beneficial influence on the severity of chondral lesion in osteoarthritis rats. This treatments could related to a reduced level of chondrocyte apoptosis through anti-apoptotoc capacities of MIA-induced apoptotic protein overexpression.
Keywords
Achyrantis radixs; Ultrasound; Osteoarthritis; Cartilage;
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