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Apoptotic Effect of Vitisin A from Vitis Amurensis against MES-SA Uterine Cancer Cells  

Lim, Jeong-Han (Graduate School of East-West Medical Science, Kyunghee University)
Lee, Hyo-Jeong (College of Oriental Medicine, Kyunghee University)
Lee, Eun-Ok (College of Oriental Medicine, Kyunghee University)
Lee, Hyo-Jung (College of Oriental Medicine, Kyunghee University)
Kwon, Hee-Young (College of Oriental Medicine, Kyunghee University)
Shim, Bum-Sang (College of Oriental Medicine, Kyunghee University)
Ahn, Kyoo-Seok (College of Oriental Medicine, Kyunghee University)
Kim, Sung-Hoon (Graduate School of East-West Medical Science, Kyunghee University, College of Oriental Medicine, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.2, 2008 , pp. 290-295 More about this Journal
Abstract
The cytotoxic characteristics of Vitsin A isolated from Vitis amurensis L. were examined in human colorectal, breast, uterine and renal cancer cells. Vitsin A showed good cytotoxicity against various cancer cells with $IC_{50}$ of $1\;{\sim}\;30\;{\mu}M$. Among them, Vitisin A exhibited strongest cytotoxic effect against MES-SA cells with $IC_{50}$ of 1.11 ${\mu}M$ by SRB assay. To verify whether the cytotoxicity of Vitisin A may be associated with apoptosis, TdT-mediated-dUTP Nick-End Labeling (TUNEL) assay and cell cycle analysis were performed in MES-SA cells. Apoptotic bodies were observed in Vitisin A treated MES-SA cells by TUNEL assay. Also, Vitisin A effectively increased the portion of $sub-G_1$ DNA content by flow cytometric analysis. Taken together, these findings suggest that the cytotoxicity of Vitisin A against MES-SA cells is chiefly mediated by apoptosis.
Keywords
Vitisin A; MES-SA; apoptosis; cytotoxicity; TUNEL; sub-G1 DNA;
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