Browse > Article

Effect of Gucheokbogol-tang in Proliferation and Calcification of Osteoblast  

Han, Deok-Hee (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Kang, Hee-Jeong (Department of Microbiology, High Technology, Dankook University)
An, Joung-Jo (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Jo, Hyun-Kyung (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Yoo, Ho-Rhyong (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Seol, In-Chan (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Kim, Yoon-Sik (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.1, 2008 , pp. 142-147 More about this Journal
Abstract
This study was purpose to investigate that Gucheokbogol-tang(GBT), mainly used in osteoporosis, has possible regulatory effect on bone regeneration by operating in proliferation and calcification of osteoblast. So that, their cytotoxicity and proliferation was investigated with MTT by ELISA, and morphological change was observed by optical microscope and electron microscope. And activation of alkaline phosphates(ALP) which is secreted in early stage of bone formation was measured, and accumulation of $Ca^{2+}$ in the process of calcification was investigated by alizaline red S assay(ARS). The results were as follows: The MG-63 cell, originated from human cell was activated in $10^{-5}$ treated group, and the level of ALP was also increased in the treated group, highest on the third day. And from the outcome of ARS assay of calcification process in the Gucheokbogol-tang(GBT) treated group for 21days. These results suggested that Gucheokbogol-tang(GBT) is effective in proliferation and calcification of osteoblast.
Keywords
osteoporosis; osteoblast; calcification$Ca^{2+}$; MG-63; ALP; Gucheokbogol-tang(GBT);
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kartsogiannis, V., Ng, K.W. Cell lines and primary cell cultures in the study of bone cell biology. J. Mol. Endocrinol. 228: 79-102, 2004   DOI
2 이인우, 최진규. 홍화씨 건강법. 태일 출판사, pp 45-70, 1998
3 Roth, J.A., Kim, B.G., Lin, W.L., Cho, M.I. Melatonin promotes osteoblast differentiation and bone formation. J. Biol. Chem. 274: 22041-22047, 1999   DOI
4 Anselme, K., Broux, O., Noel, B., et al. In vitro control of human bone marrow stromal cells for tissue engineering, Tissue Eng. 8: 941-953, 2002   DOI   ScienceOn
5 Oh, K.O., Kim, S.W., Kim, J.Y., Ko, S.Y., Kim, H.M., Baek, J.H., Ryoo, H.M., Kim, J.K. Effect of Rehmannia glutinosa Libosch extracts on bone metabolism. Clinica Chimica Acta 334: 185-195, 2003   DOI
6 Gregory, C.A., Gunn, W.G., Peister, A., Prockop, D.J. An Alizarin red-based assay of mineralization by adherent cells in culture : comparison with cetylpyridinium chloride extraction. Anal. Biochem. 329: 77-84, 2004   DOI   ScienceOn
7 Ling, L.J., Ho, F.C., Chen, Y.T., Holborow, D.W., Liu, T.Y., Hung, S.L. Areca nut extracts modulated expression of alkaline phosphatase and receptor activator of nuclear factor kB ligand in osteoblasts. J. Clin Periodontol 32: 353-359, 2005   DOI   ScienceOn
8 Yao, K.L., Todescan, R., Sodek, J. Temporal changes in matrix protein synthesis and mRNA expression during mineralized tissue formation by adult rat bone marrow cells in culture. J. Bone Miner. Res pp 231-240, 1994
9 Simonet, W.S., Lacey, D.L., Dunstan, C.R. et al. Osteoprotegerin : a novel secreted protein involved in the regulation of bone density. Cell, 89: 309-319, 1997   DOI   ScienceOn
10 Beertsen, W., Theo, V.D.B. Calcification of dental collagen by cultured rabbit periosteum : The role of alkaline phosphatase. Matrix 9: 159-171, 1989   DOI   ScienceOn
11 강정구, 유형근, 신형식. 홍화씨 추출물이 치주인대세포와 조골유사세포의 골 광물화 작용에 미치는 효과. 대한치주과학학회지 28: 475-489, 1998
12 본초학. 동의학연구소, pp 319-320, 1993
13 Stanford, C.M., Jacobson, P.A., Eanes, E.D., Lembke, L.A., Midura, R.J. Rapidly forming apatitic mineral in an osteoblastic cell line (UMR 106-01 BSP). J. Biol. Chem. 270: 9420-9428, 1995   DOI   ScienceOn
14 유윤정, 이현정, 임소형, 강정화, 이은희, 옥승호, 최봉규, 전길자. 녹용 추출물에 의한 MC3T3 세포의 분화촉진. 대한치주과학회지 30: 885-892, 2000
15 두진수, 강정구, 유형근, 신형식. 생약제제가 세포활성도에 미치는 효과. 대한치주과학회지 27: 459-468, 1997
16 Tuck, S.P., Francis, R.M. Osteoporosis. J. Postgrad Med 78: 526, 2002   DOI   ScienceOn
17 Lacey, D.L., Timms, E., Tan, H.L., et al. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell, 93: 165-176, 1998   DOI   ScienceOn
18 Barling, P.M., Gupta, D.K., Lim, C.E. Involvement of phosphodiesterase I in mineralization: histochemical studies using antler from red deer(Cervus elaphus) as a model. Calcif. tissue Int. 65: 384-389, 1999   DOI   ScienceOn
19 Wennberg, C., Hessle, L., Lundberg, P., Mauro, S., Narisawa, S., Lerner, U.H. et al. Functional characterization of osteoblasts and osteoclasts from alkaline phosphatase knockout mice. J. Bone Miner. Res 15: 1879-1888, 2000   DOI   ScienceOn
20 Feng, J.Q., Chen, D., Esparza, J., Harris, M.A., Mundy, G.R., Harris, S.E. Deer antler tissue contains two types of bone morphogenetic protein 4 m RNA transcripts. Biochim. Biophys Acta 1263: 163-168, 1995   DOI   ScienceOn