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Hepatoprotective Effect of Ethanol Extract of xBrassicaraphanus on Liver Injury in Rats Treated by D-galactosamine  

Rhee, Yun-Hee (Oriental Medical College, Kyunghee University)
Lee, Eun-ok (Oriental Medical College, Kyunghee University)
Lee, Hyo-Jung (Oriental Medical College, Kyunghee University)
Shim, Bum-Sang (Oriental Medical College, Kyunghee University)
Ahn, Kyoo-Seok (Oriental Medical College, Kyunghee University)
Choi, Jong-Won (College of Pharmacy, Kyongsung University)
Lee, Soo-Seong (Biobreeding Institute Chungang University)
Yoon, Byong-Su (Department of Biology, Kyonggi University)
Kim, Sung-Hoon (Oriental Medical College, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.6, 2007 , pp. 1388-1393 More about this Journal
Abstract
The protective effect of xBrassicoraphanus (BR) on liver inhury was evaluated in the rats with liver injury induced by i.p. injection of D-galactosamine (GalN) following 2 week oral treatment of ethanol extract of xBrassicoraphanus (EBR). EBR (200 mg/kg) significantly suppressed the levels of ALT, AST, SDH, ${\gamma}-GT$, ALP, LDH and lipid peroxidation compared with GalN treated control, while EBR at 100 mg/kg significantly suppressed AST and ${\gamma}-GT$. Similarly, EBR at 200 mg/kg significantly attenuated the levels of Phase I enzymes such as XO, AO, AH and AD as well as significantly increased the levels of Phase II enzymes such as SOD, catalase and GSH-Px in the GalN treated rats. Taken together, these results indicate that the ethanol extract of xBrassicoraphanus may have a hepatoprotective effect against GalN induced liver injury, suggesting the ethanol extract of xBrassicoraphanus can be applied as hepatoprotective functional food. However, its mechanism should be further studied in molecular and cellular view points.
Keywords
xBrassicoraphanus; galactosamine; liver injury; hepatoprotective effect;
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1 Lee, J.M. Vegetable Horticultures. Hynagmunsa. pp 324- 347, 2003
2 Tan, W., Lin, D.X., Xiao, Y., Kadlubar, F., Chen, J.S. Chemoprevention of 2-amino-1-methyl-6-phenyli-midazo 4,5-b pyridine-induced carcinogen-DNA adducts by Chinese cabbage in rats. World J. Gastroenterol. 5(2):138-142, 1999   DOI
3 Chaturvedi, P., George, S., Machacha, C.N. Protective role of Raphanus sativus root extract on paracetamol-induced hepatotoxicity in albino rats. Int. J. Vitam. Nutr. Res. 77(1):41-45, 2007   DOI
4 Lowry, O.H., Rodebrough, N.J. Farr, A.L., Randall, R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem.. 193: 265, 1951
5 이정규, 김나영, 한용남, 최종원. 홍삼의 전처리에 의한 사염화탄소 및 갈락토사민 유발 간독성에 대한 보호효과. 고려인삼학회지 27(1):1-10, 2003
6 이효정, 김관형, 이은옥, 최종원, 강경선, 윤병수, 김성훈. Chinese cabbage 잎과 뿌리가 메탄올층의 bromobenzene 간 손상에 대한 보호효과. 동의생리병리학회지 17(5):1177-1181, 2003
7 El-Mofty, S.K., Scrutton, M.C., Serroni, A., Nicolini, C., Farber K.L. Early, reversible plasma membrane injury in galctosamine-induced liver cell death. Am. J. Pathol. 79: 579-595, 1975
8 Spiteller, G. The important role of lipid peroxidation processes in aging and age dependent diseases. Mol. Biotechnol. 37(1):5-12, 2007   DOI   ScienceOn
9 Niki, E., Yoshida, Y., Saito, Y., Noguchi, N. Lipid peroxidation: mechanisms, inhibition, and biological effects. Biochem. Biophys. Res. Commun. 338(1):668-676, 2005   DOI   ScienceOn
10 Takaya, Y., Kondo, Y., Furukawa, T., Niwa, M. Antioxidant constituents of radish sprout (Kaiware-daikon), Raphanus sativus L. J. Agric. Food Chem. 51(27):8061-8066, 2003   DOI   ScienceOn
11 하배진, 이상헌. 함초의 간독성에 대한 보호효과. 생명과학회지 16(1):95-100, 2006   과학기술학회마을
12 Hanlon, P.R., Webber, D.M., Barnes, D.M. Aqueous extract from Spanish black radish (Raphanus sativus L. Var. niger) induces detoxification enzymes in the HepG2 human hepatoma cell line. J. Agric. Food Chem. 55(16):6439-6446, 2007   DOI   ScienceOn
13 Morimitsu, Y., Hayashi, K., Nakagawa, Y., Horio, F., Uchida, K., Osawa, T. Antiplatelet and anticancer isothiocyanates in Japanese domestic horseradish, wasabi. Biofactors. 13(1-4):271-276, 2000   DOI   ScienceOn
14 Wang, J., Wendel, A. Studies on the hepatotoxicity of galactosamine endotoxin or galactosamine/TNF in the perfused mouse liver. Biochem. Pharmacol. 39: 267-270, 1990   DOI   ScienceOn
15 Decker, K., Keppler, D. Galactosamine hepatitis: key role of the nucleotide deficiency period in the pathogenesis of cell injury and cell death. Rev. Physiol. Biochem. Pharmacol. 71: 77-106, 1974   DOI
16 Luczaj, W., Skrzydlewska, E. The present-day look at lipid peroxidation. Postepy Biochem. 52(2):173-179, 2006
17 염태환 역편. 방약합편, 의약사, p 163, 1973
18 Lee, S.S. Breeding a fertile intergeneric allotetraploid plant between heading Chinese cabbage and korean radish. Kor. J. Hort. Sci. Technol. 10: 653, 1999
19 Gutierrez, R.M., Perez, R,L. Raphanus sativus (Radish): their chemistry and biology. Scientific World J. 4: 811-837, 2004   DOI
20 Katayama, S. Oxidative stress marker. Nippon Rinsho. 64(S) 6: 142-146, 2006