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Apoptotic Cell Death by Methanol Extract of Phellinus linteus-Barley Corn in Human Leukemic U937 Cells through Induction of p21 and Bax, and Activation of Caspase-3  

Park, Cheol (Department of Oriental Medicine, College of Oriental Medicine, Dong-Eui University)
Kim, Hyun-Joog (Department of Oriental Medicine, College of Oriental Medicine, Dong-Eui University)
Chung, Kyung-Tae (Department of Biomedical Laboratory Science, College of Natural Science, Dong-Eui University)
Yoon, Tae-Kyung (Environmental Engineering Major, College of Engineering, Dong-Eui University)
Choi, Byung-Tae (Department of Anatomy, Pusan National University Graduate School of Oriental Medicine)
Lee, Yong-Tae (Department of Oriental Medicine, College of Oriental Medicine, Dong-Eui University)
Park, Dong-Il (Department of Oriental Medicine, College of Oriental Medicine, Dong-Eui University)
Choi, Yung-Hyun (Department of Oriental Medicine, College of Oriental Medicine, Dong-Eui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.5, 2007 , pp. 1226-1232 More about this Journal
Abstract
Phellinus linteus is a well-known Oriental medicinal fungus that has various biological activities, including immunomodulatory and anti-tumor activities, the mechanisms of which are poorly understood. In the present study, we investigated the anti-proliferative activity of the methanol extract of P. linteus-Barley corn (MEPLB) in human lekemic U937 cells. It was found that exposure of U937 cells to MEPLB resulted morphological change and growth inhibition in a dose-dependent manner as measured by trypan blue count and MTT assay. Upon treatment with MEPLB, U937 cells developed many of the hallmark features of apoptosis, including condensation of chromatin and an increase in the sub-G1 population suggesting that the anti-proliferative effect of MEPLB is associated with the induction of apoptosis. The anti-proliferative and apoptotic effects of MEPLB were connected with a marked induction of the pro-apoptotic Bax and cyclin-dependent kinase (Cdk) inhibitor p21 in a p53-independent manner. Additionally, MEPLB treatment significantly induced the caspase-3 activity in U937 cells. Taken together, the present results suggest that apoptotic signals evoked by MEPLB in human leukemic U937 cells may converge caspase-3 activation through an up-regulation of Bax rather than a down-regulation of Bcl-2 or Bel-xL.
Keywords
Phellinus linteus; U937; Barley corn; apoptosis; p21; Bax;
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