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Experimental Study of Patholobi Caulis on the Transient Cerebral Ischemia in Rats  

Lee, Sang-Lok (College of Oriental Medicine, Dongshin University)
Choi, Chan-Hun (College of Oriental Medicine, Dongshin University)
Baek, Jin-Ung (College of Oriental Medicine, Dongshin University)
Youn, Dae-Hwan (College of Oriental Medicine, Dongshin University)
Jeong, Sang-Hun (College of Oriental Medicine, Woosuk University)
Han, Ung (College of Oriental Medicine, Woosuk University)
Jeong, Hyun-Woo (College of Oriental Medicine, Dongshin University)
Kim, Gye-Yeop (Department of Physical Therapy, Dongshin University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.5, 2007 , pp. 1127-1134 More about this Journal
Abstract
The study was designed to investigate the mechanism of Patholoobi Caulis freeze dried powder (PCF) on the regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats and cytokines production ($IL-1{\beta}$, $TNF-{\alpha}$, IL-10, $TGF-{\beta}$) in cerebral ischemic rats. The results in normal rats were as follows ; Increase of PCF-induced rCBF was significantly inhibited by pretreatment with methylene blue (10 ${\mu}g/kg$, I.p.), an inhibitor of guanylate cyclase, and was inhibited by indomethacin (1 mg/kg, i.p.), an inhibitor of cyclooxygenase. Increase of PCF-induced MABP was decreased by pretreatment with methylene blue, but was increased by indomethacin. These results suggested that the mechanism of action PCF was mediated by cyclic 3',5'-guanosine monophosphate. The results in cerebral ischemic rats were as follows ; In cytokine production in serum from femoral arterial blood 1 hr after middle cerebral arterial occlusion, PCF (10 mg/kg. i.p.) significantly decreased $IL-1{\beta}$ and $TNF-{\alpha}$ production, and increased IL-10 production compared with control group. In cytokine production in serum from femoral arterial blood 1 hr 1 hr after reperfusion, PCF (10 mg/kg, i.p.) significantly decreased $IL-1{\beta}$ production, and incresed IL-10 production compared with control group. These results suggested that PCF was significantly and stably increased regional cerebral blood flow by inhibiting $IL-1{\beta}$ production, and by accelerating IL-10 production.
Keywords
Patholobi Caulis; regional cerebral blood flow (rCBF); mean arterial blood pressure (MABP); cytokines ($IL-1{\beta}$; , $TNF-{\alpha}$; , IL-10, $TGF-{\beta}$; ); cyclic 3',5'-guanosine monophosphate;
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1 全國韓醫科大學 本草學敎授 共編. 本草學, 永林社, 서울, pp 445- 446, 1999
2 鄭虎占. 中藥現代硏究與應用, 學苑出版社, 北京, pp 2539- 2546, 1998
3 김상호, 문형배, 서재홍, 정동규, 정상우. 일반병리학, 고문사, 서울, pp 146-147, 1995
4 이금수, 정현우, 강성용. 석창포가 백서의 뇌연막동맥의 직경에 미치는 기전연구, 대한본초학회지 15(2):1-7, 2000
5 崔貞植, 宋泰元, 金東熙. 鷄血藤이 collagen으로 유발된 생쥐의 關節炎 抑制에 관한 硏究, 大韓本草學會誌 18(3):79-88, 1003
6 Longa, E.Z., Weinstein, P.R., Carlson, S., Cummins, R. Reversible middle cerebral artery occlusion without craniectomy in rats, Stroke 20(1):84-91, 1989   DOI   ScienceOn
7 Okura, Y., Takeda, K., Honda, S., Hanawa, H., Watanabe, H., Kodama, M., Izumi, T., Aizawa, Y., Seki, S., Abo, T. Recombinant murine interleukin-12 facilitates induction of cardiac myosin-specific type 1 helper T cells in rats, Circ Res. 82(10):1035-1042, 1998   DOI   ScienceOn
8 Kim, S.J., Romeo, D., Yoo, Y.D., Park, K. Transforming growth factor-beta ; expression in normal and pathological conditions, Horm. Res. 42(1-2):5-8, 1994   DOI   ScienceOn
9 Kloen, P., Gebhardt, M.C., Perez-Atayde, A., Rosenberg, A.E., Springfield, D.S., Gold, L.I., Mankin, H.J. Expression of transforming growth factor-beta(TGF-beta) isoforms in osteosarcomas ; TGF-beta3 is related to disease progression, Cancer. 80(12):2230-2239, 1997   DOI   ScienceOn
10 吳馨淑. 鷄血藤 抽出物이 생쥐의 造血 및 免疫作用에 미치는 영향, 대전대학교 대학원, 2000
11 김경환. 이우주의 약리학 강의(제4판), 의학문화사, 서울, pp 82, 120-124, 432-442, 146, 355, 397, 404, 1998
12 Schmidt-Kastner, R.K., Freund, T.F. Selective vulnerability of the hippocampus in brain ischemia, Neuroscience 40: 599-636, 1991   DOI   ScienceOn
13 Garcia, J.H., Liu, K.F., Yoshida, Y., Lian, J., Chen, S., del Zoppo, G.J. Influx of leukocytes and platelets in an evolving brain infarct(Wistar rat), Am J. Pathol. 144: 188-199, 1994
14 Vila, N., Castillo, J., Davalos, A., Esteve, A., Planas, A.M., Chamorro, A. Levels of anti-inflammatory cytokines and neurological worsening in acute ischemic stroke, Stroke 34(3):671-675, 2003   DOI   ScienceOn
15 Schubert, J., Brill, W.A. Antagonism of experimental cyanide toxicity in relation to the in vivo activity of cytochrome oxidase, J. Pharmacol. Exp. Ther. 162(2):352-359, 1968
16 Kang, I.C., Kim, S.A., Song, G.Y., Beak, N.I., Park, Y.D., Ryu, S.Y., Saiki, I., Kim, S.H. Effects of the ethyl acetate fraction of Patholobi caulis on tumor cell aggregation and migration, Phytother. Res. 17: 163-167, 2003   DOI   ScienceOn
17 강성용, 이원석, 정현우, 한종현. 청피의 실험적 연구(I), 대한방제학회지 8(1):329-342, 2000
18 Wang, C.X., Olschowka, J.A. and Wrathall, J.R. Increase of interleukin-1beta mRNA and protein in the spinal cord following experimental traumatic injury in the rat, Brain Res. 759(2):190-196, 1997   DOI   ScienceOn
19 김인세. 뇌허혈과 뇌보호, 대한마취과학회지 33: 195-203, 1997
20 Cain, B.S., Meldrum, D.R., Dinarello, C.A., Meng, X., Joo, K.S., Banerjee, A., Harken, A.H. Tumor necrosis factor-alpha and interleukin-1beta synergistically depress human myocardial function, Crit Care Med. 27(7):1309-1318, 1999   DOI   ScienceOn
21 정현우. 자음건비탕加지각․천마가 뇌허혈 동물의 뇌혈류역학 변동 개선에 미치는 작용기전, 동의생리병리학회지 17(5): 1194-1201, 2003
22 Schubert, P., Morino, T., Miyazaki, H., Ogata, T., Nakamura, Y., Marchini, C., Ferroni, S. Cascading glia reactions ; a common pathomechanism and its differentiated control by cyclic nucleotide signaling, Ann. N. Y. Acad Sci. 903: 24-33, 2000   DOI
23 宋政錫, 鄭鉉雨. 祛風導痰湯이 白鼠의 腦血流變化에 미치는 機轉硏究, 東醫生理病理學會誌 16(1):99-103, 2002
24 Palmer, P.M.J., Ferrige, A.G. and Monacada, S. Nitric oxide release accounts for the biology activity of endothelium derived relaxing factor, Nature 327: 524-526, 1990   DOI   ScienceOn
25 Shin, H.K., Shin, Y.W., Hong, K.W. Role of adenosine A(2B) receptors in vasodilation of rat pial artery and cerebral blood flow autoregulation, Am J. Physiol Heart Circ Physiol. 278(2):339-344, 2000   DOI
26 Hergruido, M.J., Carceller, F., Roda, J.M. and Avendano, C. Hippocampal cell loss in transient global cerebral ischemia in rat ; A critical assessment, Neuroscience 93: 71-80, 1999   DOI   ScienceOn
27 Okamoto, H., Ito, O., Roman, R.J., Hudetz, A.G. Role of inducible nitric oxide synthase and cyclooxygenase-2 endotoxin-induced cerebral hyperemia, Stroke 29(6):1209-1218, 1998   DOI   ScienceOn
28 Vila, N., Castillo, J., Davalos, A., Esteve, A., Planas, A.M., Chamorro, A. Levels of anti-inflammatory cytokines and neurological worsening in acute ischemic stroke, Stroke 34(3):671-675, 2003   DOI   ScienceOn
29 심관섭, 김진화, 이동환, 박성민, 표형배, 장영희, 이범천. 계혈등 추출물의 항산화와 사람 피부 섬유아세포에서의 Matrix Metalloproteinase-1 발현저해 효과, 한국생물공학회지 20(1):40-45, 2005   과학기술학회마을
30 Iadecola, C., Zhang, F., Xu, X. SIN-1 reverses attenuation of hypercapnic cerebrovasodilation by nitric oxide synthase inhibitors, Am J. Physiol. 267(1Pt 2):228-235, 1994
31 Stepaniak, J.A., Gould, K.E, Sun, D., Swanborg, R.H. A comparative study of experimental autoimmune encephalomyelitis in Lewis and DA rats, J. Immunol. 155: 2762-2769, 1995
32 Trembaly, L.F., Valenza, S.P. and Ribelro, J.L. Injurious ventilatory strategiea increase cytokines and c-fos mRNA expression in an isolated rat lung model, J. Clin. Invest 99(5):944-952, 1999   DOI   ScienceOn
33 하대유 외 25인. 면역학, 고문사, 서울, pp 100-105, 1994
34 이광주, 정희원. 임상신경학, 고려의학, 서울, pp 128-131, 203-204, 1997
35 이상록, 정현우. 계혈등이 뇌혈류량 및 Lactate Dehydrogenase 활성에 미치는 실험적 효과, 동의생리병리학회지 20(1):25-30, 2006   과학기술학회마을
36 Chen, S.T., Hsu, C.Y., Hogan, E.L., Maricque, H., Balentine, J.D. A model of focal ischemic stoke in the rat : reproducible extension cortical infarction, Stroke 17: 738-743, 1986   DOI   ScienceOn
37 Bakalova, R., Matsuura, T., Kanno, I. The cyclooxygenase inhibitors indomethacin and Rofecoxib reduced regional cerebral blood flow evoked by somatosensory stimulation in rats, Exp. Biol. Med. 227(7):465-473, 2002   DOI
38 Abe, K., Aoki, M., Kawagoe, J., Yoshida, T., Hattori, A., Kogure, K., Itoyama, Y. Ischemic delayed neuronal death ; A mitochondrial hypothesis, Stroke 26(8):1478-1489, 1995   DOI   ScienceOn
39 대한병리학회. 병리학. 고문사, 서울, pp 33-34, 87-99, 127-129, 452-455, 475-484, 1263-1264, 1994
40 의과대학 신경해부학교수 편. 증례를 통한 임상신경해부학. 한우리, 서울, p 191, 1998
41 Wang, Q., Pelligrino, D.A., Paulson, O.B. and Lassen, N.A. Comparison of the effects of NG-nitro-L-arginine and indomethacin on the hypercapnic cerebral blood flow increase in rats, Brain Res. 641(2):257-264, 1994   DOI   ScienceOn
42 Yamamoto, S., Nishizawa, S., Yokoyama, T., Ryu, H., Uemura, K. Subarachnoid hemorrhage impairs cerebral blood flow response to nitric oxide but not to cyclic GMP in large cerebral arteries, Brain Res. 757(1):1-9, 1997   DOI   ScienceOn
43 Hayashi, Y., Jikihara, I., Yagi, T., Fukumura, M., Ohashi, Y., Ohta, Y., Takagi, H., Maeda, M. Immunohistochemical investigation of caspase-1 and effect of caspase-1 inhibitor in delayed neuronal death after transient cerebral ischemia, Brain Res. 893(1-2):113-120, 2001   DOI   ScienceOn
44 박철훈, 배인태, 정현우. 팔물탕이 뇌혈류역학 변화에 미치는 효과, 동의생리병리학회지 18(4):1014-1020, 2004
45 Rivera, D.L., Olister, S.M., Liu, X., Thompson, J.H., Zhang, X.J., Pennline, K., Azuero, R., Clark, D.A., Miller, M.J. Interleukin-10 attenuates experimental fetal growth restriction and demise, FASEB J. 12(2):189-197, 1998   DOI
46 Iwamoto, J., Yoshinaga, M., Yang, S.P., Krasney, E. and Krasney, J. Methylene blue inhibits hypoxic cerebral vasodilation in awake sheep, J. Appl Physiol. 73(6):2226-2232, 1992   DOI
47 Petito, C.K., Feldmann, E., Pulsinelli, W.A., Plum, F. Delayed hippocampal damage in humans following cardiorespiratory arrest, Neurology 37(8):1281-1286, 1987   DOI
48 Di Santo, E., Sironi, M., Pozzi, P. Interleukin-10 inhibits lipopolysaccharide-induced tumor necrosis factor and interleukin-1 beta production in the brain without affecting the activation of the hypothalamus-pituitary-adrenal axis, Neuroimmunomodulation 2(3):149-154, 1995   DOI   ScienceOn
49 李宇彬. 抗癌中藥藥理與應用, 黑龍江科學技術出版社, 黑龍江, pp 745-747, 1999
50 Wang, W., Wang, J., Zhao, D., Liu, H., Zhou, W., Chen, K.: Comparison of Spatholobus suberectus Dunn, Euonymus alatus (Thunb.) Sied and Eupolyphaga sinensis Walker on regulation of plasma lipid, Zhongguo Zhong Yao Za Zji 16: 299-301, 1991
51 Hallenbeck, J.M., Dutka, A.J., Tanishima, T., Kochanek, P.M., Kumaroo, K.K., Thompson, C.B., Obrenovich, T.P., Contreras, T.J. Polymorphonuclear leukocyteaccumulation in brain region with low blood flow during the early postischemic period, Stroke 17: 246-253, 1986   DOI   ScienceOn
52 Daddi, N., Suda, T., D'Ovidio, F., Kanaan, S.A., Tagawa, T., Grapperhaus, K., Kozower, B.D., Ritter, J.H., Yew, N.S., Mohanakumar, T., Patterson, G.A. Recipient intramuscular cotransfection of naked plasmid transforming growth factor beta1 and interleukin 10 ameliorates lung graft ischemia-reperfusion injury, J. Thorac Cardiovasc Surg. 124(2):259-269, 2002   DOI   ScienceOn
53 김희성, 이상록, 정현우. 육군자탕이 뇌허혈에 미치는 억제효과, 동의생리병리학회지 18(2):419-426, 2004
54 常敏毅. 抗癌本草, 湖南科學技術出版社, 長沙, pp 171-172, 1987
55 이경은, 김경환. 허혈, 재관류 손상에서 뇌조직 아민 변동과 Free Radical과의 관련성, 大韓神經科學會誌 8(1):2-8, 1990
56 임광모, 박철훈, 정현우. 자음건비탕加지각․천마가 뇌세포 및 뇌혈류역학 변동에 미치는 영향, 동의생리병리학회지 17(1):64-70, 2003
57 차배천, 이은희, 노미애. 계혈등의 항산화 활성, 생약학회지 36(1):50-55, 2005   과학기술학회마을
58 趙學敏. 本草綱目拾遺. 商務印書館香港分館, 香港, pp 260- 262, 1986
59 Lei, Y., Zhen, J., Ming, X.L., Jian, H.K. Induction of higher expression of IL-beta and TNF-alpha, lower expression of IL-10 and cyclic guanosine monophosphate by pulmonary arterial hypertension following cardiopulmonary bypass, Asian J. Surg. 25(3):203-208, 2002   DOI   ScienceOn
60 김병회, 백승희. 鷄血藤이 자궁경부암세포의 세포자멸사 유도와 관련 유전자 발현에 미치는 영향, 대한한방부인과학회지 18(1):169-180, 2005
61 박영선, 백승희. 鷄血藤의 beta-sitosterol 성분이 자궁근종세포의 증식억제와 세포자멸사의 유동에 미치는 영향, 대한한방부인과학회지 18(1):181-191, 2005
62 金天中, 趙秀仁, 鄭鉉雨. 淸暈化痰湯이 局所腦血流量에 미치는 實驗的 硏究, 東醫生理病理學會誌 16(2):316-321, 2002
63 i, R.W., Lin, G.D., Myers, S.P., Leach, D.N. Anti-inflammatory activity of Chinese medicinal vine plants, J. Ethnopharmacol. 85: 61-67, 2003   DOI   ScienceOn
64 Shibuki, K. and Okada, D. Endogenous nitric oxide release required for long term synaptic depression in the cerebellum, Nature 349: 326-328, 1991   DOI   ScienceOn
65 Lobell, A., Weissert, R., Storch, M.K., Svanholm, C., de Graaf, K.L., Lassmann, H., Andersson, R,, Olsson, T., Wigzell, H. Vaccination with DNA encoding an immunodominant myelin basic protein peptide targeted to Fc of immunoglobulin G suppresses experimental autoimmune encephalomyelitis, J. Exp. Med. 187(9):1543-1548, 1998   DOI   ScienceOn
66 조규선, 정승현, 신길조, 이원철. 牛黃淸心元이 중대뇌동맥 결찰로 유발된 뇌허혈에 미치는 영향, 대한한의학회지 22(1):78-89, 2001
67 김인세, 박두진, 권재영, 김해규, 백승완, 신상욱. 백서의 중대뇌동맥 폐쇄에 의한 허혈성 뇌손상 모델에서 Dexamethasone과 MK-801이 뇌부종 형성에 미치는 영향, 대한마취학회지 37: 327-333, 1999