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Effect of ${\alpha}-spinasterol$ Extracted from Phytolacca americanna on the Apoptosis of U937 cell line  

Yang, Jun-Seok (Department of Pathology, College of Oriental Medicine, Woosuk University)
Jeong, Sang-Hun (Department of Pathology, College of Oriental Medicine, Woosuk University)
Kim, Ho (Department of Pathology, College of Oriental Medicine, Woosuk University)
Han, Ung (Department of Pathology, College of Oriental Medicine, Woosuk University)
Jin, Jae-Ho (Department of Pathology, College of Oriental Medicine, Woosuk University)
Jung, Il-Kook (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Kim, Dae-Keun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Jeong, Seung-Il (Jeonju Biomaterials Institute)
Jeong, Han-Sol (Department of Pathology, College of Oriental Medicine, Woosuk University)
Lee, Kwang-Gyu (Department of Pathology, College of Oriental Medicine, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.5, 2007 , pp. 1108-1117 More about this Journal
Abstract
To investigate the possible mechanism of ${\alpha}-spinasterol$ as a candidate of anti-cancer drug, I examined the effects of ${\alpha}-spinasterol$ on the apoptosis of U937 cells MTT assay, flow cytometric analysis, SDS-polyacrylamide gel electrophoresis, Western blot analysis, and RT-PCR were performed. ${\alpha}-spinasterol$ treatment reduced the cell viablilty of U937 cells in a dose-dependent manner, which was associated with the induction of apoptotic cell death. ${\alpha}-spinasterol$ treatment also reduced the levels of Bcl-xL anti-apoptotic protein expression and increased the levels of caspase-3, p53, pro-apoptotic protein, in U937 cells. After treatment the level of Bcl-xL, anti-apoptotic gene expression was decreased and the level of ICE pro-apoptotic gene expression was increased. These findings suggest that ${\alpha}-spinasterol$ induced the apoptotic cell death via regulation of several growth regulatory gene products. The abbreviations used are: FBS, fetal bovine serum; PBS, phosphate buffered saline; PI, propidium iodide; OD, optical density; DiOC6, 3,3-dihexyloxa carbcyanine iodide; MTT, 3 [4-5-dimethylthiazol-2-yl] -2-diphenyltetrazolium bromide.
Keywords
${\alpha}-spinasterol$; apoptosis; flow cytometric analysis; caspase; p53;
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1 Richardson, M.A., Sanders, T., Palmer, J.L., Greisinger, A., Singletary, S.E. Complementary/alternative medicine use in a comprehensive cancer center and the implications for oncology. J. Clin. Oncol. 18: 2505-2514, 2000   DOI
2 Ralph, P., Moore, M.A., Nilsson, K. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J Exp Med 143: 1528-1533, 1976   DOI   ScienceOn
3 Zamzami, N., Marchetti, P., Castedo, M., Zanin, C., Vayssiere, J.L., Petit, P.X., Kroemer, G. Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo. J Exp. Med 181: 1661-1672, 1995   DOI   ScienceOn
4 Birch, D.E., Kolmodin, L., Laird, W.J., McKinney, N., Wong, J., Young, K.K.Y., Zangenberg, G.A., Zoccoli, M.A. Simplified Hot-Start PCR. Nature 381: 445-446, 1996   DOI
5 백문기, 홍경만 역. 암유전자(Geoffrey M. Cooper). 서울, 월드사이언스 p 350, 2000
6 余桂淸. 歷代中醫腫瘤案論選粹. 北京, 北京出版社, pp 1-2, 1988
7 史宇廣 等. 當代名醫臨證精華, 腫瘤全集. 北京, 中醫古籍出版社 pp 1-25, 1982
8 華陀. 中藏經 卷中. 江蘇科學技術出版社, p 45, 1985
9 儲水鑫. 惡性腫瘤中醫調理四法. 上海中醫藥雜誌 7: 33-34, 1992
10 趙健娬. 吳一純敎授治療晩期惡性腫瘤的經驗. 陜西醫學 14: 451-453, 1993
11 Fisher, D.E. Apoptosis in cancer therapy: Crossing the threshold. Cell 78: 539, 1994   DOI   ScienceOn
12 Yamazaki, R., Kusunoki, N., Matsuzaki, T., Hashimoto, S., Kawai, S. Nonsteroidal Anti-Inflammatory Drugs Induce Apoptosis in Association with Activation of Peroxisome Proliferator-Activated Receptor ${\gamma}$ in Rheumatoid Synovial Cells. J Pharmacol Exp Ther 302: 18-25, 2002   DOI
13 Srinivasula, S.M., Ahmad, M., Fernandes-Alnemri, T. and Alnemri, E.S. Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization. Mol Cell 1: 949-957, 1998   DOI   ScienceOn
14 Enari, M., Sakahira, H., Yokoyama, H., Okawa, K., Iwamatsu, A., Nagata, S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391: 43-50, 1998   DOI   ScienceOn
15 Greenblatt, M.S., Bennett, W.P., Hollstein, M., Harris, C.C. Mutations in the p53 tumor suppressor gene; clues to cancer etiology and molecular pathogenesis. Cancer Res 54: 4855-4878, 1994
16 Levine, A.J., Momand, J., Finlay, C.A. The p53 tumor suppressor gene. Nature 351: 453-456, 1991   DOI   ScienceOn
17 Johnson, L.V., Walsh, M.L. and Chen, L.B. Localization of mitochondria in living cells with rhodamine 123. Proc Natl Acad Sci USA 77: 990-994, 1980
18 Zamzami, N., Marchetti, P., Castedo, M., Decaudin, D., Macho, A., Hirsch, T., Susin, S.A., Petit, P.X., Mignotte, B., Kroemer, G. Sequential reduction of mitochondrial transmembrane potential and generation of reactive oxygen species in early programmed cell death. J Exp Med 182: 367-377, 1995   DOI   ScienceOn
19 朱震亨. 丹溪醫集. 北京, 人民衛生出版社, p 246, 1993
20 顔正華. 中藥學. 北京, 人民衛生出版社, p 270, 1991
21 Petit, P.X., O'Connor, D., Grunwald, D., Brown, S.C. Analysis of the membrane potential of rat- and mouse-liver mitochondria by flow cytometry and possible applications. Eur J Biochem 194: 389-397, 1990   DOI   ScienceOn
22 買堏. 癌瘤防治硏究. 서울, 成輔社, pp 25-28, 1984
23 Zou, H., Li, Y., Liu, X. and Wang, X. An APAF-1. cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. J Biol Chem 274: 11549-11556, 1999   DOI   ScienceOn
24 대한병리학회. 병리학. 5th ed, 서울, 고문사, p 144, 2003
25 Sakahira, H., Enari, M., Nagata, S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 391: 96-99, 1998   DOI   ScienceOn
26 이진우, 정우열. 활혈화어약물을 응용한 새로운 암치료제 개발 연구. 대한 동의병리학회지 14(1):45-50, 2000
27 Itoh, T., Kikuchi, Y., Shimizu, N., Tamura, T., Matsumoto, T. $24{\beta}$-ethyl-31-norlanosta-8,25(27),-diene-$3{\beta}$-ol and $24{\beta}$-ethyl- 25(27)-dehydrophenol in seeds of three Cucurbitaceae specise. Phytochemistry 20: 1929-1933, 1981   DOI   ScienceOn
28 金達鎬, 金重漢. 注解 補注 黃帝內經․靈樞(水脹, 九鍼論). 서울, 도서출판 의성당, p 1036, 1352, 2000
29 Yuan, J., Shaham, S., Ledoux, S., Ellis, H.M. and Horvitz, H.R. The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1-converting enzyme. Cell 75: 641-652, 1993   DOI   ScienceOn
30 Deckwerth, T.L. and Johnson, E.M. Jr. Temporal analysis of events associated with programmed cell death (apoptosis) of sympathetic neurons deprived of nerve growth factor (NGF). J Cell Biol 123: 1207-1222, 1993   DOI   ScienceOn
31 Du, C., Fang, M., Li, Y., Li, L. and Wang, X. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell 102: 33-42. 2000   DOI   ScienceOn
32 Vayssiere, J.L., Petit, P.X., Risler, Y., Mignotte, B. Commitment to apoptosis is associated with changes in mitochondrial biogenesis and activity in cell lines conditionally immortalized with simian virus 40. Proc Natl Acad Sci USA 91(24):11752-11756, 1994
33 Wyllie, A.H. Apoptosis. Br J Cancer 67: 205-208, 1993   DOI   ScienceOn
34 Liu, X., Kim, C.N., Yang, J., Jemmerson, R. and Wang, X. Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell 86: 147-157, 1996   DOI   ScienceOn
35 Wyllie, A.H., Morris, R.G., Smith, A.L., Dunlop, D. Chromatin cleavage in apoptosis association with condensed chromatin morphology and dependence on macromolecular synthesis. J Pathol 142: 67, 1984   DOI
36 金達鎬, 李鍾성. 注解 補注 黃帝內經․素問 上(陰陽別論, 腹中論篇). 서울, 도서출판 의성당, p 174, 837, 2000
37 金達鎬. 注解 補注 黃帝內經․靈樞(脈度篇). 서울, 도서출판 의성당, p 488, 2000
38 Kothakota, S., Azuma, T., Reinhard, C., Klippel, A., Tang, J., Chu, K., McGarry, T.J., Kirschner, M.W., Koths, K., Kwiatkowski, D.J., Williams, L.T. Caspase-3-Generated Fragment of Gelsolin: Effector of Morphological Change in Apoptosis. Science 278: 294-298, 1997   DOI
39 Telford, W.G., King, L.E., Fraker, P.J. Comparative evaluation of several DNA binding dyes in the detection of apoptosis associated chromatin degradation by flow cytometry. Cytometry 13: 137-143, 1992   DOI   ScienceOn
40 張介賓. 景岳全書, 대성문화사, 卷23 積聚, p 477, 1988
41 金達鎬. 注解 補注 黃帝內經․素問 上(生氣通天篇). 서울, 도서출판 의성당, p 63, 2002
42 Bradford, M.M. A rapid and sensitive method for the quatitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254, 1976   DOI   ScienceOn
43 薛己. 薛氏醫案. 上海, 上海古籍出版社, p 315, 1980
44 Liu, X., Zou, H., Slaughter, C., Wang, X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 89: 175-184, 1997   DOI   ScienceOn
45 Parkin, D.M. Global cancer statistics in the year 2000. Lancet Oncol 2: 533, 2001   DOI   ScienceOn
46 Burnette, W.N. "Western blotting" - Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112: 195-203, 1981   DOI   ScienceOn
47 Yang, E., Korsmeyer, S.J. Molecular Thanatopsis: A Discourse on the BCL2 Family and Cell Death. Blood 88: 386-401, 1996
48 杜志德. 商陸醇浸膏的促腎上腺皮質功能. 藥物分析雜誌 1: 31, 1983
49 方軍 等. 商陸皂甲抑制大鼠腹腔巨噬細胞釋放血小板活化因子. 藥學學報 26: 721, 1991
50 Jain, M.P., Koul, S.K., Dhar, K.L. and Atal, C.K. Novel nor-harmal alkaloid from Adhato davasica. Phytochemistry 19: 1880-1882, 1980   DOI   ScienceOn
51 Fields, S., Jang, S.K. Presence of a potent transcription activating sequence in the p53 protein. Science 249: 1046-1049, 1990   DOI
52 Wyllie, A.H. Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284: 555-556, 1980   DOI   ScienceOn
53 明․李時珍 著. 本草綱目(上). 서울, 一中社, p 1123, 1991
54 Furuya, T., Orihara, Y. and Hayashi, C. Triterpenoids from Eucalyptus periniana cultured cells. Phytochemistry 26: 715-719, 1987   DOI   ScienceOn
55 노성택, 김인락. 상륙이 고지방식이 흰쥐의 체지질 변화에 미치는 영향. 대한본초학회지 16(1):153-157, 2001
56 巢元方. 諸病源候論. 臺北, 文化圖書有限公司, pp 1-17, 129, 1979
57 巢元方. 諸病源候論. 北京, 人民衛生出版社, pp 576, 644, 691-692, 1979
58 Nicoletti, I., Migliorati, G., Pagliacci, M.C., Grignani, G., Riccardi, C.A. Rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 139: 271-279, 1991   DOI   ScienceOn
59 Cheng, E.H., Kirsch, D.G., Clem, R.J., Ravi, R., Kastan, M.B., Bedi, A., Ueno, K., Hardwick, J.M. Conversion of Bcl-2 to a Bax-like Death Effector by Caspases. Science 278: 1966-1968, 1997   DOI
60 Kastan, M.B., Zhan, Q., El-Deiry, W.S., Carrier, F., Jacks, T., Walsh, W.V., Plunkett, B.S., Vogelstein, B., Fornace, A.J. Jr. A mammalian cell cycle check point pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia. Cell 71: 587-597, 1992   DOI   ScienceOn
61 Petit, P.X., Lecoeur, H., Zorn, E., Dauguet, C., Mignotte, B., Gougeon, M.L. Alterations in mitochondrial structure and function are early events of dexamethasone-induced thymocyte apoptosis. J Cell Biol 130(1):157-167, 1995   DOI   ScienceOn
62 isani, P., Bray, F., Parkin, D.M. Estimates of the world-wide prevalence of cancer for 25 sites in the adult population. Int J Cancer 97: 72, 2002   DOI   ScienceOn
63 葛洪. 肘後備急方. 北京, 人民衛生出版社, pp203-210, 1987
64 Dowdy, S., Weardon, S. Statistics for research. New York, Wiley, p 262, 1983
65 Thompson, C.B. Apoptosis in the pathogenesis and treatment of disease. Science 267: 1456-1462, 1995   DOI
66 Clem, R.J., Cheng, E.H., Karp, C.L., Kirsch, D.G., Ueno, K., Takahashi, A., Kastan, M.B., Griffin, D.E., Earnshaw, W.C., Veliuona, M.A., Hardwick, J.M. Modulation of cell death by Bcl-xL through caspase interaction. Proc Natl Acad Sci USA 95: 554-559, 1998