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Effect of 2-Chloromethyl-1-Dihydroxyphosphinylpyrrolidine(2C-1DPP) on Differentiation Induction of Human Leukemia HL-60 Cells  

Kim, Youg-Mi (Division of Natural Science, College of Natural Sciences, Wonkwang University)
Ju, Seong-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Park, Jun-Ho (Division of Natural Science, College of Natural Sciences, Wonkwang University)
Oh, Jung-Mi (Division of Bio-Nano Chemistry, College of Natural Sciences, Wonkwang University)
Lee, Chae-Ho (Division of Bio-Nano Chemistry, College of Natural Sciences, Wonkwang University)
Kim, Eun-Cheol (Division of Oral and Maxillofacial Pathology, College of Dentistry, Wonkwang University)
Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Kim, Won-Sin (Division of Natural Science, College of Natural Sciences, Wonkwang University)
Kim, Won-Sin (Division of Natural Science, College of Natural Sciences, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.4, 2007 , pp. 940-945 More about this Journal
Abstract
We have examined the induction of HL-60 cell differentiation by treatment of 2-chloromethyl-1-dihydroxyphosphinyl pyrrolidine(2C-1DPP), which is derivative of piperidine and pyrrolidine by ${\alpha}-phosphoramidoakylation$ reaction. It was observed that HL-60 cell proliferation was dose- and time-dependently inhibited by treatment with 2C-1DPP. 2C-1DPP treatment caused a significant change in NBT reduction and enhanced ATRA-induced NBT reduction. Treatment of 2C-1DPP to HL-60 cells increased only CD11b expression in the cells, and also increased markedly G0/G1 stage arrest of HL-60 cells. These results can suggest that 2C-1DPP induced the differentiation of HL-60 cells to granulocytes lineage and enhanced ATRA-induced differentiation. Moreover, DNA expression levels of p27 were up-regulated during 2C-1DPP-dependent HL-60 cell differentiation. Our results suggest that 2C-1DPP have potential as a therapeutic agent in human leukemia.
Keywords
HL-60; ATAR; differentiation; p27; 2-chloromethyl; pyrrolidine;
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