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Water Extract of Flowers of Magnolia Denudata Inhibits LPS-induced Nitric Oxide and Pro-inflammatory Cytokines Production in Murine Peritoneal Macrophage by Inhibiting $NF-{\kappa}B$ Activation  

Kim, Do-Yun (Department of Herbology, College of Oriental Medicine, Wonkwang University)
Jeong, Won-Seok (Department of Herbology, College of Oriental Medicine, Wonkwang University)
Moon, Hyoung-Chul (Department of Clinical laboratory, Wonkwang University Gwangju Medical Center)
Park, Sung-Joo (Department of Herbology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.4, 2007 , pp. 916-920 More about this Journal
Abstract
Flowers of Magnolia denudata has been reported to possess a variety of pharmacological activities. In this study, we investigated the anti-inflammatory effects and mechanism of the water extract of Flowers of Magnolia denudata(MD) in lipopolysacchride (LPS)-mediated inflammatory mediators in murine peritoneal macrophages. MD itself does not have any toxic effects in murine peritoneal macrophages. MD inhibits LPS-induced nitric oxide (NO), tumor necrosis factor $(TNF)-{\alpha}$, IL-6 and IL-12 production in murine peritoneal macrophages. Furthermore, we have found that MD inhibited LPS-induced $NF-{\kappa}B$ but not c-Jun N-terminal kinase (JNK), p38 and extracellular signal-ragulated kinase (ERK) activation. These results suggested that MD inhibit LPS-induced production of $TNF-{\alpha}$, IL-6 and IL-12 via suppression of the $NF-{\kappa}B$ activation.
Keywords
Magnolia denudata; Nitric oxide(NO); $TNF-{\alpha}$; IL-12; IL-6 $NF-{\kappa}B$;
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