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Effect of Forsythiae Fructus Exract on the Release of Inflammatory Mediatorinduced by Lipopolysaccharide in RAW 264.7 Macrophage  

You, Bok-Jong (Department of Oriental Medical Ophthalmology & Otolarygology & Dermatology, College of Oriental Medicine, Semyung University)
Kim, Hee-Taek (Department of Oriental Medical Ophthalmology & Otolarygology & Dermatology, College of Oriental Medicine, Semyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.3, 2007 , pp. 765-770 More about this Journal
Abstract
Forsythiae fructus has traditionally been used for the treatment of erysipelas, skin rash and acute or chronic inflammatory disorders. The effect of Forsythiae fructus against lipopolysaccharide-induced inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), on mouse RAW 264.7 macrophages. Forsythiae fructus extract suppressed the expression of iNOS, COX-2 and NF-$_K$B mRNAs on the lipopolysaccharide-stimulated enhancement in RAW 264.7 macrophages. We examined the expression of iNOS and COX-2 in both mRNA and protein levels to investigate the mechanism by which Forsythiae fructus extract inhibits NO production. Forsythiae fructus extract significantly reduced iNOS, NF-$_K$B and PGE$_2$, but didn't inhibit COX-2 expression which was induced by LPS treatment in Raw 264.7 cells. These results suggest that Forsythiae fructus exerts anti-inflammatory effects probably by suppression of the iNOS and NF-$_K$B expressions.
Keywords
Forsythiae fructus; lipopolysaccharide; iNOS; COX-2; NF-$_K$B; PGE$_2$;
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