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Anti-hypertensive Effects of ethanol extract of Phyllostachys Pubescens via Antioxidant Activity  

Lee, Hye-Suk (Department of Physiology, College of Oriental Medicine, Dongguk University)
Park, Min-Hee (School of Food and Life Science, Biohealth Products Research Center and Food Science Institute, Inje University)
Kim, Jung-Suk (School of Food and Life Science, Biohealth Products Research Center and Food Science Institute, Inje University)
Lim, Beong-Ou (Department of Life Science, College of Biomedical & Health Science, Konkuk University)
Moon, Gap-Soon (Department of Life Science, College of Biomedical & Health Science, Konkuk University)
Shin, Heung-Mook (Department of Physiology, College of Oriental Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.3, 2007 , pp. 658-665 More about this Journal
Abstract
Phyllostachys pubescens (Maengiong-Juk), a kind of the bamboo, was reported to have many beneficial pharmacological actions. in this study, of using 70% ethanol extract of Phyllostachys pubescens we investigated its efficacy on angiotensin converting enzyme (ACE) and antioxidant enzyme activities. In addition, vasorelaxant effect was examined in rat aortic rings. The inhibitory effect of ACE activity by Phyllostachys pubescens extract (PPE) was dose-dependently increased by 61.42% at 10mg/ml. PPE relaxed the pre-contracted rat aortic rings with 10$^{-6}$M phenylephrine, showing about 88% at 4.0mg/ml. Sprague Dawley (SD) rats were given different concentrations of PPE mixed in the drinking water for 10 weeks. PPE did not show any difference with control group in blood pressure, body weight (BW) and food intake. However, it revealed the highest total antioxidative effect at dose of 1.0 g/100 g BW in plasma by TEAC assay. Thiobarbituric acid reactive substance (TBARS) and protein carbonyl levels which are markers of tissue peroxidation, were significantly lowed at the same dosage. Furthermore, hepatic antioxidant enzymes such as total superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and catalase activities were also significantly increased by PPE (1.0 g/100 g BW). In conclusion, we suggest that PPE might have antihypertensive effect through increasing antioxidant activities.
Keywords
Phyllostachys pubescens extract (PPE); nagiotensin converting enzyme (ACE); antioxidative effect; tissue peroxidation;
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