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Comparison of Immunomodualtory Effects of Water-extracted Adenophorae Radix, Liriopis Tuber, Dendrobii Herba, Polygonati Odorati Rhizoma and Polygonati Rhizoma  

Park, Si-Deok (Department of Pathology, College of Oriental Medicine, Deagu Haany University)
Lee, Geum-Hong (Department of Pathology, College of Oriental Medicine, Deagu Haany University)
Lee, Young-Sun (Department of Traditional Health Resource Development, College of Sung Duk)
Kwon, Young-Kyu (Department of Physiology, College of Oriental Medicine, Deagu Haany University)
Park, Jong-Hyun (Department of Pathology, College of Oriental Medicine, Deagu Haany University)
Choi, Sun-Mi (Department of Medical Research, Korea Institute of Oriental medicine)
Shin, Sang-Woo (Department of Pathology, College of Oriental Medicine, Deagu Haany University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.2, 2007 , pp. 414-424 More about this Journal
Abstract
Adenophorae Radix (AR), Liriopis Tuber (LT), Dendrobii Monile (DM), Polygonati Officinalis (PO), Polygonati Rhizoma (PR) have been used to treated a variety condition/diseases in traditional oriental medicine. The present study was conducted to investigate the immunmodulatory effects of the water-extracted AR, LT, DH, PO and PR. In spleen cell proliferation assay, DH was significantly enhanced mitogenic activity compared with control group. In RT-PCR, DH ad PO induced IL-2 and IFNr cytokine gene expression in mouse spleen cells. Methotrexate(MTX), immune supression agent, was significantly inhibited mouse spleen cell proliferation(1600 mg/ml). In spite of MTX treatement, DH and PO sustained the spleen cell proliferation, In the flow cytometry analysis, DH stimulated mouse spleen cells showed an increase in B-cell phenotype (CD45R/B220). The water-extracted DH and PO inhibited NO production and iNOS expression in LPS-stimulated RAW 264.7 macrophage cell. DH induced IL-2 and IFNg gene expression in human peripheral mononuclear cells. The GC-MS analysis show that the main component of water-extracted DH was b-Nitroethyl alcohol. The main components of water-extracted PO were Dipirartril-tropico, Methyl sulfoxide and Demsodrox. These data suggest that among these extracts, DH has a protective effcet of immune suppression caused by MTX. DH may be enhance cellular and humoral immune response by the regulation of cytokine gene expression, NO production and B cell production.
Keywords
Dendrobii Herba; Polygonati Odorati Rhizoma; immunomodualtory effects; methotrexate; IL-2; IFN${\gamma}$;
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Times Cited By KSCI : 1  (Citation Analysis)
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