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Protective Effects of Bojungbangam-tang Extracts on ECV304 Cell Cytotoxicity  

Kwon, Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kim, Eun-Kyung (Department of Biochemistry, Chonbuk National University Medical School)
Song, Mi-Young (Department of Biochemistry, Chonbuk National University Medical School)
Han, Mi-Jeong (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Lee, Su-Yeop (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Lee, Heon-Jae (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Lee, Young-Rae (Department of Biochemistry, Chonbuk National University Medical School)
Ju, Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Ryu, Do-Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kim, Sung-Hoon (Department of Pathology, College of Oriental Medicine, KyungHee University)
Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.2, 2007 , pp. 404-407 More about this Journal
Abstract
This study was designed to investigate the protective effect of Bojungbangam-tang Ethanol Extracts (EBJT) on cisplatin and hydrogen peroxide-induced cytotoxicity of human endothelial cell line ECV304 cells. After cells were treated with cisplatin and hydrogen peroxide, MTT assay was performed for cell viability test. To explore the mechanism of cytotoxicity, we used the several measures of apoptosis to determine whether this processes was involved in cisplatin and hydrogen peroxide-induced cell damage in ECV304 cells. Also, cells were treated with EBJT and then, followed by the addition of cisplatin or hydrogen peroxide. Cisplatin or hydrogen peroxide decreased the viability of ECV304 cells in a dose-dependent manner. ECV304 cells treated cisplatin or hydrogen peroxide were revealed as apoptosis characterized by nuclear staining. EBJT protected ECV304 cells from cisplatin or hydrogen peroxide-induced nuclear fragmentation and chromatin condensation. Also, EBJT inhibited the cleavage of poly(ADP-ribose) polymerase (PARP) in cisplatin or hydrogen peroxide-treated ECV304 cells. According to above results, EBJT may protect ECV304 cells from the apotosis induced by cisplatin or hydrogen peroxide.
Keywords
Bojungbangam-tang; cisplatin; hydrogen peroxide; lendothelial cell;
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