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Inhibitory Effect of Uncaria Sinensis on Matrix Metalloproteinase-9 Activity and Human Aortic smooth Muscle Cell migration  

Kwak, Chang-Geun (Department of Pathology, College of Oriental Medicine, Dongguk University)
Choi, Dall-Yeong (Department of Pathology, College of Oriental Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.6, 2006 , pp. 1629-1635 More about this Journal
Abstract
The migration of vascular smooth muscle cells (VSMC) and the production of matrix metallopreteinases-9 (MMP-9) may play a key role in the development of atherosclerosis. In this study, we have more extensively investigated the inhibitory effect of UR on MMP-9 activity and TNF-${\alpha}$ induced human aortic smooth muscle cells (HASMC) migration. The result from gelatin zymography showed that UR inhibited MMP-9 activity in a dose-dependent manner (IC50 = 55 g/ml). In addition, UR strongly inhibited the migration of HASMC induced by TNF-treatment (IC50 = 125 g/ml), although it has very low cytotoxic effect on HASMC (IC50 > 500 g/ml). These results suggest that UR is a potential anti-atherosclerotic agent through inhibition of MMP-9 activity and VSMC migration.
Keywords
Cho-Deung-San; atherosclerosis; Uncaria sinensis (Oliv.) Havil;
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1 Sasaguri, Y., Murahashi, N., Sugama, K., Kato, S., Hiraoka, K., Satoh, T., Isomoto, H., Morimatsu, M. Development-related changes in matrix metalloproteinase expression in human aortic smooth muscle cells. Lab. Invest. 71:261-269, 1994
2 Fitzgerald, M., Hayward, I.P., Thomas, A.C., Campbell, G.R., Campbell, J.H. Matrix metalloproteinases can facilitate the heparanase-induced promotion of phenotypic change in vascular smooth muscle cells. Atherosclerosis 145:97-106, 1999   DOI   ScienceOn
3 Ha, K.T., Lee, T.K., Kwak, K.H., Kim, J.K., Kim, D.I., Choi, D.Y., Kim, C.H. Inhibitory effect of Cho-Deung-San on human aortic smooth muscle cell migration induced by TNF-$\alpha$alpha through inhibition of matrix metalloproteinase-2 and-9 activity. Vascul. Pharmacol. 41:83-90, 2004   DOI   ScienceOn
4 Dollery, C.M., McEwan, J.R., Henney, A.M. Matrix metalloproteinases and cardiovascular disease, Circ. Res. 77:863-868, 1995   DOI   ScienceOn
5 Kim, D.W., Chung, H.J., Nose, K., Maruyama, I., Tani, T. Preventive effects of a traditional Chinese formulation, Chaihu-jia-Longgu-Muli-tang, on intimal thickening of carotid artery injured by balloon endothelial denudation in rats. J. Pharm. Pharmacol. 54:571-575, 2002   DOI   ScienceOn
6 Kleiner, D.E., Stetler-Stevenson, W.G. Matrix metalloproteinases and metastasis. Cancer chemotherapy and pharmacology 43:42-51, 1999   DOI   ScienceOn
7 Hofmann, C.S., Sonenshein, G.E. Green tea polyphenol epigallocatechin-3 gallate induces apoptosis of proliferating vascular smooth muscle cells via activation of p 53. FASEB J. 17:702-704, 2003   DOI
8 Chung, T.W., Moon, S.K., Chang, Y.C., Ko, J.H., Lee, Y.C., Cho, G., Kim, S.H., Kim, J.G., Kim, C.H. Novel and therapeutic effect of caffeic acid and caffeic acid phenyl ester on hepatocarcinoma cells: complete regression of hepatoma growth and metastasis by dual mechanism. FASEB J. 18:1670-1681   DOI   ScienceOn
9 Galis, Z.S., Johnson, C., Godin, D., Magid, R., Shipley, J. M., Senior, R.M., Ivan, E., Targeted disruption of the matrix metalloproteinase-9 gene impairs smooth muscle cell migration and geometrical arterial remodeling. Circ. Res. 91:852-859, 2002   DOI   ScienceOn
10 Mason, D.P., Kenagy, R.D., Hasenstab, D., Bowen-Pope, D.F., Seifert, R.A., Coats, S., Hawkins, S.M., Clowes, A.W. Matrix metalloproteinase-9 overexpression enhances vascular smooth muscle cell migration and alters remodeling in the injured rat carotid artery, Circ. Res. 85:1179-1185, 1999   DOI   ScienceOn
11 Zempo, N., Koyama, N., Kenagy, R.D., Lea, H.J., Clowes, A. W. Regulation of vascular smooth muscle cell migration and proliferation in vitro and in injured rat arteries by a synthetic matrix metalloproteinase inhibitor. Arterioscler. Thromb. Vasc. Biol. 16:28-33, 1996   DOI   ScienceOn
12 Jiang, M.C., Liao, C.F., Lee, P.H. Aspirin inhibits matrix metalloproteinase-2 activity, increases E-cadherin production, and inhibits in vitro invasion of tumor cells. Biochemical and biophysical research communications 282:671-677, 2001   DOI   ScienceOn
13 GalisZ, S., Muszynski, M., Sukhova, G.K., Simon-Morrissey, E., Unemori, E.N., Lark, M.W., Amento, E., Libby, P. Cytokinestimulated human vascular smooth muscle cells synthesize a complement of enzymes required for extracellular matrix digestion. Circ. Res. 75:181-189, 1994   DOI   ScienceOn
14 Tonn, J.C., Kerkau, S., Hanke, A., Bouterfa, H., Mueller, J.G., Wagner, S., Vince, G.H., Roosen, K. Effect of synthetic matrixmetalloproteinase inhibitors on invasive capacity and proliferation of human malignant gliomas in vitro. International journal of cancer 80:764-772, 1999   DOI   ScienceOn
15 Schwartz, R.S., Holmes, D.R., Topol, E.J. The restenosis paradigm revisited: an alternative proposal for cellular mechanisms. J. Am. Coll. Cardiol. 20:1284-1293, 1992   DOI
16 Brew, K., Dinakarpandian, D., Nagase, H. Tissue inhibitors of metalloproteinases: evolution, structure and function. Biochim. Biophys. Acta. 1477:267-283, 2000   DOI
17 Huang, H.C., Wang, H.R., Hsieh, L.M. Antiproliferative effect of baicalein, a flavonoid from a Chinese herb, on vascular smooth muscle cell. Eur. J. Pharmacol. 251:91-93, 1994   DOI   ScienceOn
18 Moon, S.K., Cha, B.Y., Kim, C.H. ERK1/2 mediates TNF-$\alpha$induced matrix metalloproteinase-9 expression in human vascular smooth muscle cells via the regulation of NF-B and AP-1: involvement of the Ras dependent pathway. J. Cell. Physiol. In press. 2003
19 Pauly, R.R., Passaniti, A., Bilato, C., Monticone, R., Cheng, L., Papadopoulos, N., Gluzband, Y.A., Smith, L., Weinstein, C., Lakatta, E.G. Migration of cultured vascular smooth muscle cells through a basement membrane barrier requires type IV collagenase activity and is inhibited by cellular differentiation, Circ. Res. 75:41-54, 1994   DOI   ScienceOn
20 Miura, Y., Chiba, T., Tomita, I., Koizumi, H., Miura, S., Umegaki, K., Hara, Y., Ikeda, M., Tomita, T. Tea catechins prevent the development of atherosclerosis in apoprotein E-deficient mice. J. Nutr. 131:27-32, 2001   DOI
21 Chung, T.W., Moon, S.K., Lee, Y.C., Kim, J.G., Ko, J.H., Kim, C.H. Enhanced expression of matrix metalloproteinase-9 (MMP-9) by hepatitis B virus infection into liver cells. Arch. Biochem. Biophys. 408:147-154, 2002   DOI   ScienceOn
22 Ross, R. Cell biology of atherosclerosis, Annu. Rev. Physiol. 57:791-804, 1995   DOI   ScienceOn
23 Maeda, K., Kuzuya, M., Cheng, X.W., Asai, T., Kanda, S., Tamaya-Mori, N., Sasaki, T., Shibata, T., Iguchi, A. Green tea catechins inhibit the cultured smooth muscle cell invasion through the basement barrier.Atherosclerosis 166:23-30, 2003   DOI   ScienceOn
24 Ross, R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 362:801-809, 1993   DOI   ScienceOn
25 Mandal, M., Mandal, A., Das, S., Chakraborti, T., Sajal, C. Clinical implications of matrix metalloproteinases. Mol. Cell. Biochem. 252:305-329, 2003   DOI
26 Cho, A., Reidy, M.A. Matrix metalloproteinase-9 is necessary for the regulation of smooth muscle cell replication and migration after arterial injury, Circ. Res. 91:845-851, 2002   DOI   ScienceOn
27 Chamley-Campbell, J., Campbell, G.R., Ross, R. The smooth muscle cells in culture. Physiol. Rev. 59:1-61, 1979   DOI
28 Woessner, J.F. Jr. Matrix metalloproteinases and their inhibitors in connective tissue remodeling. FASEB J. 5:2145-2154, 1991   DOI
29 Yoshie, F., Iizuka, A., Kubo, M., Komatsu, Y., Matsumoto, A., Itakura, H., Takeda, H., Matsumiya, T., Kondo, K. Protective effects of Saiko-ka-ryukotsu-borei-to (Chai-Hu-Jia-Long-Gu-Mu-Li-Tang) against atherosclerosis in Kurosawa and Kusanagi-hypercholesterolemic (KHC) rabbits. Pharmacol. Res. 43:481-488, 2001   DOI   ScienceOn
30 Bendeck, M.P., Irvin, C., Reidy, M.A. Inhibition of matrix metalloproteinase activity inhibits smooth muscle cell migration but not neointimal thickening after arterial injury. Circ. Res. 78:38-43, 1996   DOI   ScienceOn
31 Moon, S.K., Cho, G.O., Jung, S.Y., Gal, S.W., Kwon, T.K., Lee, Y.C., Madamanchi, N.R., Kim, C.H. Quercetin exerts multiple inhibitory effects on vascular smooth muscle cells: role of ERK1/2, cellcycle regulation, and matrix metalloproteinase-9. Biochem. Biophys. Res. Commun. 301:1069-1078, 2003   DOI   ScienceOn
32 Kim, B.J., Kim, Y.K., Park, W.H., Ko, J.H., Lee, Y.C., Kim, C.H. A water-extract of the Korean traditional formulation Geiji-Bokryung-Hwan reduces atherosclerosis and hypercholesteremia in cholesterol-fed rabbits. Int. Immunopharmacol. 3:723-734, 2003   DOI   ScienceOn
33 Cha, B.Y., Park, C.J., Lee, D.G., Lee, Y.C., Kim, D.W., Kim, J.D., Seo, W.G., Kim, C.H. Inhibitory effect of methanol extract of Euonymus alatus on matrix metalloproteinase-9. J Ethnopharmacol. 85:163-167, 2003   DOI   ScienceOn
34 Demeule, M., Brossard, M., Page, M., Gingras, D., Beliveau, R. Matrix metalloproteinase inhibition by green tea catechins. Biochimica et Biophysica Acta 1478:51-60, 2000   DOI
35 Moon, S.K., Cha, B.Y., Kim, C.H. In vitro cellular aging is associated with enhanced proliferative capacity, G1 cell cycle modulation, and matrix metalloproteinase-9 regulation in mouse aortic smooth muscle cells. Arch. Biochem. Biophys. 418:39-48, 2003   DOI   ScienceOn
36 Kenagy, R.D., Vergel, S., Mattsson, E., Bendeck, M., Reidy, M.A., Clowes A.W. The role of plasminogen,plasminogen activators, and matrix metalloproteinases in primate arterial smooth muscle cell migration. Arterioscler. Thromb. Vasc. Biol. 16:1373-1382, 1996   DOI   ScienceOn
37 Newby, A.C., Zaltsman, A.B., Molecular mechanisms in intimal hyperplasia. J. Pathol. 190:300-309, 2000   DOI   ScienceOn
38 Cho, A., Graves, J., Reidy, M.A., Mitogen-activated protein kinases mediate matrix metalloproteinase-9 expression in vascular smooth muscle cells. Arterioscler. Thromb. Vasc. Biol. 20:2527-2532, 2000   DOI   ScienceOn
39 Matrisian, L.M. Metalloproteinases and their inhibitors in matrix remodeling. Trends. Genet. 6:121-125, 1990   DOI   ScienceOn
40 Strauss, B.H., Chisholm, R.J., Keeley, F.W., Gotlieb, A.I., Logan, R.A., Armstrong, P.W. Extracellular matrix remodeling after balloon angioplasty injury in a rabbit model of restenosis. Circ. Res. 75:650-658, 1994   DOI   ScienceOn
41 Heber, D. Herbs and atherosclerosis.Curr. Atheroscler. Rep. 3:93-96, 2001   DOI   ScienceOn
42 Ross, R. The pathogenesis of atherosclerosis: an update. N. Engl. J. Med. 314:488-500, 1986   DOI   ScienceOn