Browse > Article

Effects of Gami-Choakwiyeum on the PPAR-${\gamma}$ in the Bronchial sthma Mouse Model  

Lee, Hai-Ja (Department of Pediatrics, Collage of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.6, 2006 , pp. 1593-1597 More about this Journal
Abstract
We hope to evaluate the effects of Gami-Choakwiyeum (GCKY) on the PPAR-${\gamma}$’ in the OVA induced asthma mouse model. Female BALB/c mice, 8 weeks of age and free of murine specific pathogens were used. Mice were sensitized by intraperitoneal injection of OVA emulsified in aluminum hydroxide in a total volume of 200 ${\mu}{\ell}$ on one day and 14 days. On 21, 22, and 23 days after the initial intraperitoneal injection of OVA, the mice were challenged using an ultrasonic nebulizer. GCKY was administered 7 times by oral gavage at 24 hour intervals fromdays 19 after intraperitoneal injection of OVA. Bronchoalveolar lavage was perfromed 72 hours after the last challenge, and total cell numbers in the BAL fluid were counted. Also, the level of PPAR-${\gamma}$ of normal and OVA-induced asthma moused with/without administration of GCKY were measured by Western blot analysis. For the histologic examination, the specimens were stained with hematoxylin 2 and eosin-Y.(H & E). Numbers of total cells were increased significantly at 72 h after OVA inhalation compared with numbers of total cells in the normal and the administration of GCKY. Especially, the increased numbers of eosinophils in BAL fluids after OVA inhalation were significantly increased. However, the numbers of eosinophils reduced by the administration of GCKY. Western blot analysis revealed that PPAR-${\gamma}$ levels in nuclear level were increased slightly after OVA inhalation compared with the levels in the normal group. After the administration of GCKY, PPAR-${\gamma}$ levels in cytosolic and nuclear levels at 72 h after OVA inhalation were markedly increased. On pathologic examination, there were many acute inflammatory cells around the alveoli, bronchioles, and airway lumen of mice with OVA-induced asthma compared with inflammatory cells in the normal group. However, acute inflammatory cells around alveoli, bronchioles, and airway lumen markedly decreased after administration of GCKY, GCKY can increase a PPAR-${\gamma}$ level and could be an effective treatment in asthma patients through the PPAR-${\gamma}$ mechanism for bronchial asthma.
Keywords
Lung; bronchus; bronchial asthma; Gami-Choakwiyeum; PPAR-${\gamma}$;
Citations & Related Records
연도 인용수 순위
  • Reference
1 대한 천식 및 알레르기 학회. 천식과 알레르기 질환. 서울, 군자출판사, pp 244-254, 2002
2 Honda, K., Marquillies, P., Capron, M., Dombrowicz, D. Peroxisome proliferator-activated receptor gamma is expressed in airways and inhibits features of airway remodeling in a mouse asthma model, J. Allergy Clin, Immunol, 113:882-888, 2004   DOI   ScienceOn
3 Faveeuw, C., Fougeray, S., Angeli, V., et al. Peroxisome proliferator-activated receptor gamma activators inhibit interleukin-12 production in murine dendritic cells. FEBS Lett, 486:261-266, 2000   DOI   ScienceOn
4 Etgen, G., Mantlo, N. PPAR ligands for metabolic disorders, Curr Top Med Chem, 3:1649-1651,2003   DOI   ScienceOn
5 이형구, 정승기. 동의폐계내과학. 서울, 민서출판사, pp 187-215, 1991
6 Asada, K., Sasaki, S., Suda, T., Chida, K., Nakamura, H. Antiinflammatory roles of peroxisome proliferator-activated receptor gamma in human alveolar macrophages, Am J Respir Crit Care Med, 169:195-200, 2004   DOI
7 Woerly, G., Honda, K., Loyens, M., et al. Peroxisome proliferator-activated receptors alpha and gamma down-regulate allergic inflammation and eosinophil activation. J Exp Med, 198:411-421, 2003   DOI   ScienceOn
8 Benayoun, L., Letuve, S. et al. Regulation of peroxisome proliferator-activated receptor gamma expression in human asthmatic airways, relationship with proliferation, apoptosis, and airway remodeling, Am. J, Respir. Crit. Care Med, 164:1487-1494, 2001   DOI   ScienceOn
9 이해자. 천식동물 모델에서 청피의 치료효과-혈관내피성장요인의 측정을 통하여. 동의생리병리학회지 17(6):1475-1478, 2003
10 Birrell, B.A., Patel, H.J., McCluskie, K. et al. PPAR-gamma agonists as therapy for diseases involving airway neutrophilia, Eur Respir J , 24:18-23,2004   DOI   ScienceOn
11 최진경, 유동열. 좌귀음의 효능에 대한 문헌적 고찰방안. 대전대학교한의학연구소 논문집. 14(1):101, 2005
12 Clark, R.B. The role of PPARs in inflammation and immunity. J Leukoc Biol, 71:388-400, 2002
13 박영주, 박은정, 이해자, 박종익. 가미좌귀음의 알레르기성 기관지 천식 반응조절효과. 18(2):31-48, 2004
14 이은미,.가미형개연교탕의 면역증강효과. 원광대학교 대학원. p 21, 2003
15 Avery August, Cynthia Mueller, Veronika Weaver, Tiffany A. Polanco, Elizabeth R. Walsh, Margherita T. Cantorna Nutrients, Nuclear Receptors, Inflammation, Immunity Lipids, PPAR, and Allergic Asthma, American Society for Nutrition, 136:695-699,2006
16 이해자. 사백산이 천식유발 백서에서 metrix metalloproteinase-9에 미치는 영향. 동의생리병리학회지 18(6):1694-1698, 2004
17 대한 소아알레르기 및 호흡기학회편. 소아 알레르기호흡기학. 서울, 군자출판사 pp 107-108, 2005
18 Harris, S.G., Phipps, R.P. The nuclear receptor PPAR gamma is expressed by mouse T lymphocytes and PPAR gamma agonists induce apoptosis. Eur J Immunol, 31: 1098-1105, 2001   DOI   ScienceOn
19 Cunard, R., Ricote, M., DiCampli, D., et al. Regulation of cytokine expression by ligands of peroxisome proliferator activated receptors, J Immunol, 168:2795-2802, 2002   DOI
20 Kwak, Y.G., Song, C.H., Yi, H.K., et al. Involvement of PTEN in airway hyperresponsiveness and inflammation in bronchial asthma, J. Clin. Invest, 111:1083-1092, 2003   DOI
21 Ricote, M., Li, A., Willson, T., Kelly, C., Glass, C. The peroxisome proliferatoractivated receptor-gamma is a negative regulator of macrophage activation, Nature, 391: 79-82, 1998   DOI   ScienceOn
22 陳大舜, 曾勇, 黃政德. 각가학설. 서울, 대성의학사, p 329, 2004
23 Woerly, G., Honda, K., Loyens, M., Papin, J. P., Auwerx, J., Staels, B., Capron, M., Dombrowicz, D. Peroxisome proliferator-activated receptors alpha and gamma down-regulate allergic inflammation and eosinophil activation, J. Exp. Med, 198:411-421,2003   DOI   ScienceOn
24 신민교. 임상본초학. 서울, 영림출판사, pp 175-177, 302-305, 599-600, 1983
25 Tom Hsun-Wei Huangs, Valentina Razmovski-Naumovski, Bhavani Prasad Kota, Diana Shu-Hsuan Lin and Basil D Roufogalis, The pathophysiological function of peroxisome proliferator-activated receptor-$\gamma$ in lung-related diseases, Respiratory Research, 6:102-110, 2005   DOI   ScienceOn
26 조영민, 정희재, 정승기, 이형구. 가미 청금강화탕 및 가미육미지황탕이 알러지 천식모델 흰쥐의 BALF내 면역세포 및 혈청 IgE에 미치는 영향. 대한한의학회지 24(3):1-10, 2003