Browse > Article

Inhibitory Effects of the Methanolic Fraction from Pueraria Radix on Hydrogen Peroxide-induced Lipid Peroxidation and Cadmium-induced Cytotoxicity (III)  

Lim, Jin-A (Department of Cosmetic Science, Wonkwang Health Science)
Kim, Yun-Ha (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Institute of Basic Natural Sciences Wonkwang University)
Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Institute of Basic Natural Sciences Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.6, 2006 , pp. 1556-1560 More about this Journal
Abstract
The effects of the methanol subfraction from Pueraria Radix on hydrogen peroxide-induced lipid peroxidation and cadmium-induced cytotoxicity were investigated in NIH 3T3 fibroblast cells. After the methanol subfraction treatment, the content of MDA induced by 600 ${\mu}g$ $H_2O_2$ significantly decreased in proportion to the subfraction concentrations as well as 50 ${\mu}M$ $CdCl_2$-induced cytotoxicity. Especially, 200 ${\mu}g/mL$ concentration of methanol subfraction was strongly shown inhibition of lipid peroxidation and detoxification of cadmium. These results suggest that the methanol subfraction from Pueraria Radix retains a potential antioxidant and protective effect against cadmium.
Keywords
Pueraria Radix; lipid peroxidation; antioxidant; cadmium; detoxification;
Citations & Related Records
연도 인용수 순위
  • Reference
1 정우일. Mouse의 간과 신장에 흡수되는 카드뮴에 대한 differential pluse stipping polarography 분석. 원광대학교 대학원 석사학위 논문, 1989
2 정경진. 산두근(山豆根)추출액이 흰쥐의 급성 카드뮴 중독에 대한 해독 효과. 원광대학교 대학원 박사논문, 1994
3 Kim, S.W., Kim, E.S., Kim, Y.S. Studies on the polysaccharide extracted from Ganderma lucidum. J. Korean Soc. Food Nutr. 24(1):147-153, 1995
4 Huh, J. Donguibogam, Vol. 3, Namsandang, Seoul, 1984
5 Oh, M.J., Lee, K.S., Son, H.Y., Kim, S.Y. Antioxidative components of Pueraria root. Korean J. Food Sci. Technol. 22:793-798, 1990
6 Lee, J.S., Lee, K.H., Jeong, J.H. Effects of extracts of Puerariae radix on lipid metabolism in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 28(1):218-224, 1999
7 Ha, J.U., Ryu, Y.K., Park, H.J. Nitrite scavenging ability and antioxidative activity of water extract and ethanol extract from Cassia tora L. and Pueraria thunbergiana. Korean J. Food Sci. Ani. Resour. 21(1):1-9, 2001
8 지정옥. 녹두(綠豆) 전탕액이 랫드의 장기 조직내 카드뮴 독성 해독에 관한 연구. 대한예방한의학회지 2(1):175-192, 1998
9 Farombi, E.O., Adaramoye, O.A., Emerole, G.O. Influence of chloramphenicol on rat hepatic microsomal components and biomarkers of oxidative stress: protective role of antioxidants. Pharmacol. Toxicol. 91(3):129-134, 2002   DOI   ScienceOn
10 Multhaup, G., Ruppert, T., Schlicksupp, A., Hesse, L., Beher, D., Masters C.L., Beyreuther, K. Reactive oxygen species and Alzheimer's disease. Biochem. Pharmacol. 54: 533-539, 1997   DOI   ScienceOn
11 Yu, R., Tan, T.H., Kong, A.T. Butylated hydroxyanisole and its metabolite tert-butylhydroquinone differentially regulate mitogen-activated protein kinases. The role of oxidative stress in the activation of mitogen-activated protein kinases by phenolic antioxidants. J. Biol. Chem. 14, 272(46):28962-28970, 1987   DOI   ScienceOn
12 Tseng, K., Chon, Y., Chang, L., Fan, L. Phamacologic studies on Radix Puerariae. I. Effects of Puerariae on blood pressure, vascular reactivity, cerebral and peripheral circulate in dogs. Chin. Med. J. 1(5):335-342, 1975
13 Guerra, M.C., Speroni, E., Brococoli, M., Cangini, M., Pasini, P., Minghett, A., Crespi-Perellino, N., Mirasoli, M., Cantelli-Forti, G., Paolini, M. Comparison between chinese medical herb Pueraria lobata crude extract and its main isoflavone puerarin antioxidant properties and effects on rat liver CYP-catalysed durg metabolim. Life Sci. 3, 67(24):2997- 3006, 2000
14 이영옥, 차철환. 白鼠의 카드뮴 중독시 마늘, D-penicillamine 및 N-acetyl-DL-penicillamine의 방어효과에 관한 연구. 고대의대 논문집 23, p 43, 1986
15 엄순택, 손동빈, 차철환. 백서(白鼠)의 카드뮴 중독시 BAL 및 DMSA와 마늘의 방어효과에 대한 비교 연구. 고대의대 논문집23 p 109, 1986
16 Blois, M.S. Antioxidant determination by the use of a stable free radical. Nature 26:1199-1200, 1958
17 YU, B.P. Cellular defenses against damage from reactive oxygen species. Physiol. Rev. 74:139-162, 1994   DOI
18 Han, S.H., Kim, J.B., Min, S.G., Lee, C.H. The effects of Puerariae radix catechins administration on liver function in carbon tetrachloride-treated rats. Korean J. Soc. Food Nutr. 24:713-720, 1995
19 Lee, S.J., Baek H.J., Lee, C.H., Kim, H.P. Antiinflammatory activity of isoflavonoids from Pueraria radix and biochanin A derivatives. Arch. Pham. Res. 17(1):31-35, 1994   DOI
20 Park, J.H., Lee, Y.H., Cho, J.K., Lee, C.H. Effects of Puerariae radix root water extracts on the antioxidative activity in rats. Korean J. Food Sci. Ani. Resour. 19(1):65-71, 1999
21 강성호. 토복령(土茯岺)추출액이 흰쥐장기에서 카드뮴독성 해독에 미치는 효과. 원광대학교 대학원 석사논문, 1995
22 Voskoboynik, A., Reznick, A.Z., Rinkevich, B. Rejuvenescence and extension of an urochordate life span following a single, acute administration of an anti-oxidant, butylated hydroxytoluene. Mech. Ageing Dev. 123(9):1203-1210, 2002   DOI   ScienceOn
23 Oliveira, C.P., Kassab, P., Lopasso, F.P., Souza, H.P., Janiszewski, M., Laurindo, F.R., Iriya, K., Laudanna, A.A. Protective effect of ascorbic acid in experimental gastric cancer: reduction of oxidative stress. World J. Gastroenterol. 9(3):446-448, 2003   DOI
24 Lee, J.M., Moehlenkamp, J.D., Hanson, J.M., Johnson, J.A. Nrf2-dependent activation of the antioxidant responsive element by tert-butylhydroquinone is independent of oxidative stress in IMR-32 human neuroblastoma cells. Biochem. Biophys. Res. Commun. 12, 280(1):286-292, 2001   DOI   ScienceOn
25 Lee, M.K., Cho, S.Y., Jang, J.Y., Choi, M.S., Jeon, S.M., Jang, M.K., Kim, M.J., Park, Y.B. Effects of Puerariae flos and Puerariae radix extract on antioxidant enzymes in ethanol- treated rats. American J. Chinese Med. 29(2):343-354, 2001   DOI   ScienceOn
26 Mossmann, T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65:55-63, 1983   DOI
27 Gao, Z., Huang, K., Xu, H. Protective effects of flavonoids in the roots of Scutellaria Baicalensis Georgi against hydrogen peroxide-induced oxidative stress in HS-SY5Y cells. Pharmacol. Res. 43(2):173-178, 2001   DOI   ScienceOn
28 Smith, M.T., Thor, H., Hartzell, P., Orrenius, S. The measurement of lipid peroxidation in isolated hepatocytes. Biochem. Pharmacol. 31:19-26, 1982   DOI   ScienceOn
29 Chung, Y.H., Han, S.H., Shin, M.K. Effects of Pueraria radix in water extract on the detoxification in rat administered with cadmium. Korean J. dietary culture 17(4):456-464, 2002
30 한두석, 유일수, 이종섭, 백승화. 마우스 장기내의 카드뮴 축적에 미치는 수종 생약의 영향. 한국독성학회지 10(2):169-174, 1994
31 손인환, 길인호, 이종섭, 이기남. 홍화추출액의 카드뮴 독성 해독효과 연구. 한국전통한의학회지 8(2):86-94, 1998
32 Kang, S.S. Applications of NMR spectroscopy in analysis of flavonoids. YAKHAK HOEJI 26(3):139-148, 1982
33 안영미. 흰주의 카드뮴 독성에 대한 부추(Allium odorum L.)의 방어효과에 관한 연구. 명지대학교 박사학위논문, 1990