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Protective Effect of Ursolic Acid from Corni fructus on the Hydrogen Peroxide-induced Damage of HEI-OC1 Auditory Cells  

Yu, Hyeon-Hee (Department of Food and Nutrition, Kunsan National University)
Seo, Se-Jeong (Department of oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, Wonkwang University, VCRC)
Hur, Jong-Moon (Department of oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, Wonkwang University, VCRC)
Park, Rae-Kil (Department of Microbiology & Vestibulocochlear Research Center, School of Medicine, Wonkwang University, VCRC)
So, Hong-Seob (Department of Microbiology & Vestibulocochlear Research Center, School of Medicine, Wonkwang University, VCRC)
Jeon, Byung-Hun (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University)
You, Yong-Ouk (Department of oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, Wonkwang University, VCRC)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.6, 2006 , pp. 1524-1529 More about this Journal
Abstract
The fruits of Cornus officinalis have been used in traditional Oriental medicine for treatment of inner ear diseases, such as tinnitus and hearing loss. In the present study, we showed that the ursolic acid obtained from Corni fructus protected HEI-OC1 auditory cells from hydrogen peroxide cytotoxicity in a dose-dependent fashion. In addition, to investigate the protection mechanism of ursolic acid on hydrogen peroxide cytotoxicity toward HEI-OC1, we measured the effects of ursolic acid on lipid peroxidation and activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) in hydrogen peroxide treated cells. Ursolic acid (0.05 - 2 ${\mu}g/ml$) had protective effect against the hydrogen peroxide-induced HEI-OC1 cell damage and reduced lipid peroxidation in a dose-dependent manner. Pre-treatment with ursolic acid significantly attenuated the decrease in activities of CAT and GPX, but SOD activity was not affected by the ursolic acid or hydrogen peroxide. These results indicate that ursolic acid protects hydrogen peroxide-induced HEI-OC1 cell damage through inhibition of lipid peroxidation and induce the antioxidant enzymes CAT and GPX.
Keywords
ursolic acid; Corni fructus; superoxide dismutase; catalase; glutathione peroxidase; HEI-OC1 cell;
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