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Danchunhwan Protects the Cytotoxicity of Beta-amyloid in SH-SY5Y Neuroblastoma Cells  

Yu, Bong-Sun (Department of internal Medicine School of Oriental Medicine)
Kim, Jin-Kyung (Department of Microbiology School of Medicine, Wonkwang University)
Park, Chan-Ny (Department of internal Medicine School of Oriental Medicine)
So, Hong-Seob (Department of Microbiology School of Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.6, 2006 , pp. 1516-1523 More about this Journal
Abstract
The water extract of Danchunhwan(DCH) has been traditionally used for treatment of dementia damage in oriental medicine. However, little is known about the mechanism by which the water extract of DCH rescues cells from neurodegenerative disease such as Alzheimer's disease. This study was designed to investigate the protective mechanisms of DCH on ${\beta}$-amyloid or $H_2O_2$-induced cytotoxicity in SH-SY5Y neuronblastoma cells. ${\beta}$-amyloid and $H_2O_2$ markedly decreased the viability of SH-SY5Y cells, which was characterized with apparent apoptotic features such as membrane blebbing as well as fragmentation of genomic DNA and nuclei. However, the water extract of DCH significantly reduced both ${\beta}$-amyloid or $H_2O_2$-induced cell death and apoptotic characteristics through reduction of intracellular peroxide generation. Also, the water extract of DCH prevented prevented the mitochondrial dysfunction including the disruption of mitochondria membrane permeability transition (MPT) and the perturbation in Bcl-2 family protein expressions in $H_2O_2$-treated SH-SY5Y cells.
Keywords
Danchunhwan; neurodegenerative disease; protective effect; mitochondrial dysfunction;
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