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Comparison of Immune Promotion Effects of Water-extracted Angelicae gigantis, Rehmanniae Radix, Paeoniae japonica and Polygoni multiflori Radix  

Lee, Geum-Hong (Department of Biology, College of Natural Sciences, Kyungpook National University)
Kang, Shin-Sung (Department of Biology, College of Natural Sciences, Kyungpook National University)
An, Won-Gun (Department of Nanomedical Engineering, College of Nanoscience & Nanotechnology, Pusan National University)
Lee, Young-Sun (Department of Traditional Health Resource Development, College of Sung Duk)
Kwon, Young-Kyu (College of Oriental Medicine, Daegu Haany University)
Shin, Sang-Woo (College of Oriental Medicine, Daegu Haany University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.6, 2006 , pp. 1507-1515 More about this Journal
Abstract
Angelica gigantis (AG), Rehamaninae Radix(RR), Paenia japonica (PJ), and Polygoni multiflori Radix (PM) have been used as medicinal plants to tonify the blood. General function of the drugs have been known to nourish blood and control the heart and liver meridians. Recently, several studies have proposed mechanisms by which some oriental medicinal herbs work on the immune system. However, it is uncertain whether aqueous-extract of these drugs has immunomodulatory effect yet. In this study, I investigated the immune promotive effects of the water-extracted AG, RR, PJ and PM. The water-extracted AG, RR, PJ and PM inhibited NO production and iNOS expression in LPS stimulated RAW 264.7 macrophage cell line. Among these extracts, AG and PM induced expression of IL-2 and IFN${\gamma}$ in mouse spleen cells. In the flow cytometry analysis, PM-stimulated mouse spleen cells showed an increase in B-cell phenotype (CD45R/B220). The oral administration of Polygoni multiflori water-extracts to mice having S-180 abdominal dropsy cancer prolonged life-span more than control mice. These data suggest that among these extracts, PM has cellular and humoral immune-enhancement effect through IL-2 and IFN${\gamma}$ cytokine production, the regulation of NO production in macrophage cells and the B cell production in spleen cells.
Keywords
tonfiy the blood; immune promotion; IL-2; IFN${\gamma}$; NO; iNOS; CD45R/B220;
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